59 results about "Bacillus altitudinis" patented technology

The invention discloses a bacillus altitudinis strain and application thereof. The bacillus altitudinis strain is classified and named as Bacillus altitudinis, the preservation unit is the China general microbiological culture collection center (CGMCC), the preservation date is December 28th in 2018, the preservation number is CGMCCNo.17046. The bacillus altitudinis strain belongs to the genus ofbacillus, the family of bacillaceae, the order of bacillales, the class of bacilli, the phylum of firmicutes in the domain of bacteria. The bacillus altitudinis strain can be applied in biological control over root rot of plukenetia volubilis.

The invention discloses a strain of bacillus altitudinis and application thereof in control of tomato root-knot nematode. The bacillus altitudinis strain YT2002 has been deposited in the China GeneralMicrobiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15213; experimental results show that the bacillus altitudinis strain YT2002 biological agenthas a good effect on control of tomato root-knot nematode, with the control effect of 63.03% after 14 days after the last application; from growth of the tomato, the bacillus altitudinis strain YT2002 has an obvious effect on promoting tomato growth, and is safe and reliable without risk of damage.

The invention discloses bacillus altitudinis YLX-5 separated from a microbial mat sediment in a percolating water system in the selenium mining area of Enshi, Hubei, China and application of bacillus altitudinis YLX-5. The bacillus altitudinis YLX-5 is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number of CCTCC No: M2013675. The bacillus altitudinis YLX-5 has the characteristics of selenium-addicted microbes and has a potential application value in microbial organic selenium conversion, microbial nano-selenium synthesis, selenium-containing ore microbial activation, selenium-rich microbial fertilizer preparation and selenium-contaminated environment remediation.

The invention discloses bacillus altitudinis and application thereof to prevention and control of fusarium graminearum. The collection number of the disclosed bacillus altitudinis is CGMCC No. 9502. As a biological prevention and control material of wheat scab, the bacillus altitudinis has a very good application prospect on the aspect of development of novel bio-pesticides or biological prevention and control microbial agents.

The invention relates to a high salt-tolerant bacillus alitudinis with functions of heterotrophic nitrification-aerobic denitrification and phosphorous removal and application thereof to wastewater treatment. The bacillus alitudinis has strong high salt environment tolerance, grows well under a high-salt condition, and can metabolize by taking organic carbon as one and only carbon source and ammonia nitrogen as one and only nitrogen source; through the heterotrophic nitrification-aerobic denitrification, the ammonia nitrogen is directly converted into a gaseous product so as to achieve a denitrifying purpose; the bacillus alitudinis can also take nitrate nitrogen as one and only nitrogen source, the nitrate nitrogen is converted into a gaseous product through the aerobic denitrification; and under aerobic conditions inorganic phosphorus can be taken into a body and converted into a component of the body so as to realize a purpose of removing phosphorus in sewage. The bacillus alitudinis is applied to high-salt wastewater treatment, can achieve synchronization removal of nitrogen and phosphorus under a single aerobic condition, and provides a new way for solving a problem of biological removal of the nitrogen and phosphorus under the high-salt condition.

The invention provides Ochrobactrum guangzhouense P2 with high capacity in degrading pyridine. The strain as a novel species of Ochrobactrum genus is obtained by separating and purifying sludge of oil and gas factories, and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 207191. The strain has higher capacity in degrading pyridine, and can effectively degrade pyridine in sludge, thus lowering the sludge processing cost.

The invention discloses bacillus altitudinis SWY137 and an application thereof. The preservation number of the strain SWY137 is CCTCC NO:M 2019842, and the strain SWY137 is salt-tolerant bacillus altitudinis. The bacillus altitudinis can grow well in a 13% NaCl high-salt solution, can dissolve organic phosphorus and inorganic phosphorus which are difficult to dissolve, and can also produce variousgrowth promoting substances of cellulases, proteases, growth hormones and the like. Corn is taken as an example, the growth promoting effect of the strain SWY137 on plants is obvious, the stem heightof corn seedlings can be increased by 27.67%, the root length can be increased by 32.23%, the fresh weight of stems can be increased by 51.33%, the fresh weight of roots can be increased by 60.67%, the dry weight of the stems can be increased by 33.33%, and the dry weight of the roots can be increased by 34.88%; and besides, the effects of the strain SWY137 for promoting growth and increasing yield are not influenced by application of chemical fertilizers, and the strain SWY137 has important significance in saving phosphate fertilizer resources, enabling food grains to be subjected to continuous yield increase and protecting ecological environment of farmlands for agricultural production of China.

The invention belongs to the technical field of biology, and particularly relates to screening and application of an L-proline high-producing Brevundimonas sp. JNPP-1. An L-proline high-producing pseudomonad JNPP-1 is screened out for the first time; and by combining morphological characteristics and physiological biochemical characteristics, the strain JNPP-1 is identified as a Brevundimonas sp. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.3828. The strain can produce glucoses into L-proline, and the yield can be up to 32.67g / L. The L-proline produced by the strain is one of important amino acids for synthesizing human proteins, is an important material for amino acid transfusion, and has been widely used in multiple fields, such as food, medicine and other industries.

The invention discloses a preparation method of a composite bacterial agent for sludge composting and application thereof. The preparation method comprises the following steps: separately culturing bacillus altitudinis, purple streptomyces variabilis, bacillus stearothermophilus, bacillus subtilis and bacillus licheniformis, then mixing according to a volume ratio of bacterial liquid to obtain mixed bacterial liquid, then inoculating a fermentation medium with the mixed bacterial liquid, and performing enlargement culture to obtain the composite bacterial agent for sludge composting. The composite bacterial agent is mainly applied to high-temperature sludge composting, raises the fermentation temperature fast, maintains high temperature for a long time, can shorten the composting time, reduces the moisture content of a composted material, is low in production cost, environmentally friendly, pollution-free, excellent in performance and high in efficiency, and has relatively good economic benefits and social benefits.

The invention discloses a bacterial preparation for increasing oral sweetness and improving oral comfort during combustion and smoking of tobacco, and a tobacco product. The bacterial preparation comprises bacillus velezensis YBN3, bacillus altitudinis Y120 and enterobacter hormaechei Y399, wherein the accession numbers of the above three bacterial strains in the China General Microbiological Culture Collection Center are respectively CGMCC No. 15625, CGMCC No. 15626 and CGMCC No. 15627. The bacterial preparation disclosed by the invention is sprayed on the surface of a tobacco raw material; and after fermentation, the oral sweetness of the tobacco raw material during combustion and smoking can be obviously increased and the oral comfort of the tobacco raw material is integrally improved.

The invention discloses a solid composite microbial inoculant for promoting aerobic composting of kitchen waste as well as preparation and application of solid composite microbial inoculant. The solid composite microbial inoculant is prepared from bacillus altitudinis, alcaligenes faecalis, lysinibacillus capsici, Bacillus velezensis, alcaligenes aquae, providencia, Bacillus paramycoides, bacillus licheniformis, bacillus thuringiensis, liquid culture media and bran. The solid-state microbial compound inoculant can shorten the temperature rise time of kitchen waste compost and prolong the high-temperature period time, so that the compost can quickly reach the thoroughly decomposed and harmless standards, and the condensation and aromatization of humus are promoted. In addition, the abundance of beneficial bacterium bacillus in the composting process of the kitchen waste is also remarkably improved.

The invention discloses bacillus altitudinis having a phosphate-solubilizing function and application thereof. The bacillus altitudinis is preserved in China Center for microbial culture collection management committee general microbiology on 18th, March, 2019, of which the preservation number is CGMCC No.17338. The bacillus altitudinis preferably adopts an LB liquid culture medium used in culturing of laboratory liquid and a Mongina inorganic phosphate solid culture medium used in phosphate-solubilizing bacteria. According to the bacillus altitudinis having a phosphate-solubilizing function and application thereof provided by the invention, an experiment proves that effective phosphate concentration in blank control solutions of uninoculated bacteria is much less than that in fermented liquid of bacillus altitudinis SM01, that is, the bacillus altitudinis SM01 is an efficient phosphate-solubilizing fungus. A strain of the bacillus altitudinis disclosed by the invention has the characteristics of being high in growth speed, high in phosphate-solubilizing capability, high in stress resistance, and high in capability of quickly and massively colonizing on rhizosphere of plants, and has a good application prospect.

The invention relates to the technical field of control of rhizoctonia solani, in particular to a bacillus altitudinis strain (bacillus altitudinis) DSQN-1 of which the preservation number is CGMCC No.17236, and the strain as a biological control bacterium is used for performing biological control on the rhizoctonia solani. The bacillus altitudinis strain disclosed by the invention has better control effects on the rhizoctonia solani; and experiment is performed by a three-point face-to-face-culturing method, and the result indicates that the inhibition zone of the bacillus altitudinis (bacillus altitudinis) DSQN-1 on the rhizoctonia solani is 27.33mm.

The invention belongs to the technical field of plant disease biocontrol, and discloses a strain of bacillus altitudinis XJGS-4 and an application thereof in prevention and treatment of peronophythoralitchii. The bacillus altitudinis is named as bacillus altitudinis XJGS-4, is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) at Guangdong Institute of Microbiology, 5 floor, No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou City, has the preservation number of GDMCC No:60698, and is preserved on June 17, 2019. The obtained bacillus altitudinis comes from rhizosphere soil of healthy litchi chinensis sonn fruit trees, is mutually symbiotic with litchi chinensis sonn for a long time, and avoids the possibility of damaging of the stability of a micro ecological environment on a plant surface; the bacillus altitudinis and a biological preparation based on the bacillus altitudinis have a strong inhibitory effect on peronophythora litchii, and have agood prospect of development and application.

The invention discloses an ochrobactrum anthropi M11 for degrading unsymmetrical dimethylhydrazine and a method for degrading the unsymmetrical dimethylhydrazine by using the ochrobactrum anthropi M11. The method comprises the steps of: inoculating a strain of the ochrobactrum anthropi M11 to a slant culture medium, and culturing to obtain a slant culture product; inoculating the slant culture product into a container in which a seed culture medium is arranged, and culturing to obtain a seed liquid; immobilizing the seed liquid to obtain immobilized mycelial pellets; and inoculating the immobilized mycelial pellets into a container in which a substrate solution is filled according to an inoculation amount of 10-30%, mixing, and degrading for 72 hours at a temperature of 30-37 DEG C and the oscillation rate of 120-200r / min, wherein the inoculation amount is calculated based on the volume proportion of the seed liquid to the substrate solution. According to the invention, the degrading rate for the unsymmetrical dimethylhydrazine is high up to 97.7%.

The invention belongs to the technical field of agricultural microbe and also relates to the technical field of tobacco leaf ageing. The invention discloses a microbial highland bacillus altitudinis D2 strain which is preserved in China Center for Type Culture Collection, the preserving number is CCTCCNO: m2011056, the invention further discloses the application of the highland bacillus altitudinis D2 strain in artificial ageing of tobacco leaves at the upper part of flue-cured tobacco. The highland bacillus altitudinis is fragtant adding and upgrading dominant bacteria separated from the surfaces of tobacco leaves, can ensure artificial ageing to tobacco leaves at the upper part of flue-cured tobaccom, shortens the aging time of the tobacco leaves at the upper part, improves the sucking quality of tobacco leaves at the upper part of flue-cured tobaccom, increases aroma quality and concentration of aroma, and reduces excitant.

The invention discloses bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew. The bacillus altitudinis is bacillus altitudinis (bacillus altitudinis) YC-9, the preservation number of the bacillus altitudinis YC-9 in the General Microbiological Center of the Chinese Microbiological Culture Collection and Management Committee is CGMCC No.20884, the bacillus altitudinis can inhibit the growth of fungi and reduce the mildew of tobacco leaves, and the fermentation appearance quality of cigars can be improved by combining the bacillus altitudinis with bacillus velezensis (Bacillus velezensis) ACCC02735. It is shown that the bacillus altitudinis (bacillus altitudinis) YC-9 and the strain combination can be used for improving the quality of the tobacco leaves in the fermentation process.

The invention discloses a composite microbial agent for cassava residue matrix fermentation and a preparation method and application thereof. The composite microbial agent for cassava residue matrix fermentation comprises bacillus altitudinis, bacillus licheniformis, streptomyces griseorubens and trichoderma viride. The composite microbial agent for cassava residue matrix fermentation can accelerate temperature increase in the early stage of cassava residue fermentation, increase the fermentation temperature and prolong the time of the high-temperature period. The decomposition degree of a fermented product is reduced, the toxic effect on plants is reduced, and the fermentation quality is improved. The method has important scientific significance and application value on cassava residue matrix utilization.

The invention discloses a high-violacein-yield Janthinobacterium lividum and application thereof. The Janthinobacterium lividum is named ZSJ, and is collected by China General Microbiological Culture Collection Center on July 16th, 2014; the collection number is CGMCC No.9465; and the Janthinobacterium lividum can generate violacein after being subjected to fermentation culture. The fermentation culture medium is composed of the following solutes: 8.0-12.0 g / L peptone, 2.0-4.0 g / L powdered beef, 4.0-6.0 g / L sodium chloride and 0.5-1.5 g / L glucose. The pH value of the fermentation culture medium is 4-8; and the temperature of the fermentation culture is 7-30 DEG C, and the time is 48-240 hours. The violacein secreted by the strain belongs to a pure natural pigment, does not have the potential hazards of the artificial pigment, has broad-spectrum antibacterial, antiviral, antitumor and other bioactivities, and can be widely used in the fields of medicine, industry and the like.

The invention relates to an efficient kitchen waste degradation compound microbial agent and a preparation method and application thereof, the compound microbial agent comprises a degradation microbial agent and a deodorization microbial agent, and the degradation microbial agent comprises bacillus velezensis spores, bacillus cereus spores and bacillus altitudinis spores; the deodorizing microbial agent comprises bacillus subtilis spores and bacillus amyloliquefaciens spores. The strains are independently separated and preserved, and have excellent capability of degrading protein, fat, starch and cellulose, and the bacillus subtilis and the bacillus amyloliquefaciens also have the capability of efficiently removing NH3 and H2S. The composite microbial agent provided by the invention can quickly degrade the kitchen garbage, no odor is generated, the final product is a high-quality organic fertilizer, and harmless, reduction and resource utilization of the kitchen garbage is realized.

The invention discloses Bacillus altitudinis J54 and application thereof. The Bacillus altitudinis is Bacillus altitudinis J54, is preserved in the China Center for Type Culture Collection, and has apreservation number of CCTCC No. M2019667. Separation is performed on tobacco leaves, and then screening and purification are performed so as to obtain the Bacillus altitudinis J54; through processesof fermentation, inoculation and degradation, nitrosamine (TSNAs) specific in tobaccos can be degraded effectively in the process of tobacco leaf processing or pipe tobacco preparation, and the degradation rate can reach 5.2-46.6%, so that the quality of the tobacco leaves is improved; and the bacterial strain has good resistance to stress, easy acquisition, convenient use and broad market application prospects.

The invention relates to bacillus altitudinis and application thereof. The bacillus altitudinis is preserved in China General Microbiological Culture Collection Center on June 23, 2020, the sample name is bacillus altitudinis 181-7, the preservation number is CGMCC No.20132, and the strain is separated from a soil sample of a capsicum land in a meadow harbor garden in Haikou City in Hainan province in China. The bacillus altitudinis 181-7 disclosed by the invention shows a bacteriostatic effect on Xanthomonas oryzae pv. Oryzae and Xanthomonas oryzae pv. Oryzae, and also has a bacteriostatic effect on banana bacterial wilt pathogen, bean wilt pathogen, soybean scab pathogen and the like and some pathogenic fungi such as botrytis cinerea, phytophthora pathogen and fusarium graminearum and the like. Results show that the strain has broad-spectrum antibacterial ability, provides good biocontrol resources for preventing and treating rice bacterial leaf blight and bacterial streak, and lays a foundation for excavating novel microbial agents.

The invention relates to a microbial composition, in particular to a microbial composition with a deodorization effect. The microbial composition consists of Bacillus altitudinis (GAU-00532), Bacillus subtilis subsp. Stercoris (GAU-00533) and Bacilluszhangzhouensis (GAU-00534), the Bacillus altitudinis (GAU-00532), the Bacillus subtilis subsp. Stercoris (GAU-00533) and the Bacilluszhangzhouensis (GAU-00534) are all collected in the China Center for Type Culture Collection, the collection address is Wuhan University, Wuhan, and the collection numbers are respectively CCTCC NO: M 2021352, CCTCC NO: M 2021350 and CCTCC NO: M2021351. The desulfurization and ammonia removal microbial deodorant is low in cost, small in dosage and high in deodorization efficiency, and is free of chemical additives and complex control systems, and deodorization products can be directly utilized and do not need to be treated again.

The invention relates to the field of biological detection, and specifically relates to a typhoid bacillus colloidal gold method detection kit, and a preparation method and application thereof. The invention provides the typhoid bacillus colloidal gold method detection kit, which comprises a test paper card. The test paper card comprises a bottom plate, and a sample pad, a gold label pad, a nitrocellulose membrane and an absorbent pad which are sequentially arrayed from a sample adding end on the surface of the bottom plate; a recombined typhoid bacillus antigen and rat IgG are contained on the gold label pad; the nitrocellulose membrane is coated with detection lines and quality control lines; the recombined typhoid bacillus antigen and the rat IgG on the gold label pad adopt colloidal gold to label. The typhoid bacillus colloidal gold method detection kit provided by the invention is used for detecting typhoid bacillus through a colloidal gold immunochromatography technique for the first time, has both sensitivity and specificity, and has the advantages of quickness, simplicity and convenience in operation, accuracy in result, economic benefit, applicability and the like.

The invention discloses bacillus altitudinis ST15 antagonizing xanthomonas oryzae and application of the bacillus altitudinis ST15. The bacillus altitudinis ST15 is preserved in China General Microbiological Culture Collection Center on June 28th, 2020, and the preservation number of the strain is CGMCC No.20156. The strain has relatively high antagonistic activity to Xanthomonas oryzae pv .oryzaeand Xanthomonas oryzae pv .oryzicola, can promote the growth of rice and improve the drought resistance of rice seedlings, and has relatively strong tolerance to common chemical bactericides for preventing and treating rice bacterial diseases. Bacterial suspension or a biocontrol microbial agent prepared from the strain has a relatively high biocontrol effect on rice bacterial blight and bacterial leaf streak, can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, and has relatively high economic and social benefits.

The invention discloses a high-yield high-heat-stability bacillus licheniformis bacterial strain of beta-glucanase. The bacterial strain is obtained via the composite mutagenesis of ultraviolet-lithium chloride-sulphuric acid diethyl ester on an original bacterial strain. The bacillus licheniformis (Bacillus licheniformis) beta-lactamase 10-25 is preserved in China Center for Type Culture Collection, and the preservation number of the bacillus licheniformis is CCTCC NO: M2013538. The enzyme activity of the beta-glucanase produced by the bacterial strain is as high as 8000-10000 u / ml and is 2.5 times of that of the original bacterial strain. The enzyme activity of the beta-glucanase is higher than that of the existing beta-glucanase, the optimum pH value range of enzyme action is wide, the heat stability and the storage stability are both higher, and the bacillus licheniformis of beta-glucanase is especially suitable for industrialized demands with high reaction temperature and concurrent liquefaction technology and saccharification technology.

The present invention particularly discloses a bacillus altitudinis YC602 strain and use of the bacillus altitudinis YC602 strain. The preservation number of the bacillus altitudinis YC602 strain is GDMCC No.61221. The potassium-dissolving bacterium YC602 is separated from rhizosphere soil of potatoes in Huizhou city in Guangdong province. The bacillus altitudinis is preliminarily identified by adopting a 16SrRNA and a rpob sequence analysis method. The bacteria can dissolve insoluble potassium feldspar, improve germination of plant seeds and growth of root systems, and promote growth of plants. Besides, the strain has a function of producing auxin (IAA), has remarkable resistance to Cu (II), Cd (II), Zn (II) and Al (III) metal stress and acid stress, and has important significance in agricultural production.

The invention discloses broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 2, 2019, and the address is the fifth floor of the 59# building of the 100# courtyard of the Xianlie Middle Road of Guangzhou City; and the preservation number of the strain is GDMCC NO: 60910. According to the application of the bacillus altitudinis ZKY02 in preparation of a preparation for preventing and treating phytopathogens, the strain has good plate antagonistic activity on a variety of plant pathogenic fungi, and especially has good antagonistic ability on soil-borne vascular bundle diseases caused by a variety of fusarium.