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Bacillus altitudinis and application thereof to prevention and control of fusarium graminearum

A technology of Bacillus highlandii and Fusarium, which is applied in the directions of application, bacteria, fungicides, etc., can solve the problems of increasing difficulty in control and lack of ideal high-resistant wheat varieties, and achieve the effect of good application prospects.

Active Publication Date: 2015-02-11
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Over the years, the control of wheat head blight has mainly relied on chemical pesticides, but with the environmental problems caused by chemical pesticides, coupled with years of drug use has led to the emergence of pathogenic bacteria resistant Fusarium graminearum strains (Chang jun Chen et al., 2007) , making it more difficult to control
Although a lot of energy has been invested in related research at home and abroad on the quality of scab resistance, there is still no ideal high-resistant wheat variety (Li Zhenghui et al., 2007).

Method used

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  • Bacillus altitudinis and application thereof to prevention and control of fusarium graminearum
  • Bacillus altitudinis and application thereof to prevention and control of fusarium graminearum
  • Bacillus altitudinis and application thereof to prevention and control of fusarium graminearum

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, the isolation and identification of bacteria

[0031] 1. Isolation of bacteria

[0032] (1) In June 2012, in an ultra-clean workbench, every 10 ears of wheat diseased ears collected in Jiangsu were shaken in 100 ml of sterile distilled water for 15 minutes to prepare a bacterial suspension, and then treated in a water bath at 80°C for 10 minutes.

[0033] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, The strains with different colors and transparency were streaked and purified on the plate, and the purified strains were applied to the confrontation culture experiment of Fusarium graminearum. The obtained strain with strong antagonistic ability to Fusarium graminearum was named JS60J.

[0034] 2. Identification

[0035] (1) According to the method...

Embodiment 2

[0042] Embodiment 2, the antagonism of bacillus highlander JS60J to Fusarium graminearum

[0043] The antagonism of Bacillus highlander JS60J to Fusarium graminearum was detected by confrontation culture method.

[0044] 1. The cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG was cultured with a hole puncher with a diameter of 5 mm. 1 Take the plate from the edge of the colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0045]2. Inoculate the same volume and concentration of JS60J on a four-dot dash line at a distance of 25mm from the center of the PDA plate. Take the PDA plate only inoculated with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is any antibacterial effect bands formed. Do three parallels for each group. The results are shown in Table 1. Show that, JS60J has very strong inhibitory effect to Fusarium graminearum ( figure 1 ). ...

Embodiment 3

[0049] Embodiment 3, the inhibitory effect of JS60J volatile gas to Fusarium graminearum

[0050] The antagonism of the volatile gas produced by Bacillus highlander JS60J to Fusarium graminearum was detected by confrontation culture method.

[0051] 1. The cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG was cultured with a hole puncher with a diameter of 5 mm. 1 Take the plate from the edge of the colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0052] 2. Inoculate JS60J in the center of the NA plate, upside down on the plate connected with Fusarium graminearum, take the PDA plate only inoculated with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is antibacterial effect Effect. Do three parallels for each group. The result is as figure 2 shown. It shows that JS60J can produce volatile gas that inhibits Fusarium graminearum.

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Abstract

The invention discloses bacillus altitudinis and application thereof to prevention and control of fusarium graminearum. The collection number of the disclosed bacillus altitudinis is CGMCC No. 9502. As a biological prevention and control material of wheat scab, the bacillus altitudinis has a very good application prospect on the aspect of development of novel bio-pesticides or biological prevention and control microbial agents.

Description

technical field [0001] The invention relates to a highland bacillus strain and its application in preventing and treating Fusarium graminearum. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is one of the important pathogens of gramineous crops, which can cause wheat head blight (Fusarium Head Blight, FHB). Fusarium graminearum is a major factor limiting wheat yield (Dubin, 1997) . my country's climatic conditions are very favorable for the occurrence of wheat head blight, and the perennial disease in the middle and lower reaches of the Yangtze River caused a yield loss of 10%-15%, and a production reduction of nearly 50% in a pandemic year (Huang Chang et al., 2000). Due to the influence of global warming and changes in farming systems such as straw returning to the field and no-tillage cultivation, the occurrence area of ​​wheat head blight in my country has rapidly expanded from the Yangtze River Basin to Northwest and North China (Zhang Hao, 2...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01G7/06C12R1/10
CPCA01G7/06A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/10
Inventor 刘阳赵月菊邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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