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Bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew

A technology of Bacillus highlandi and inoculum, which can be applied in the application, bacteria, fungicides and other directions, can solve the problems of high production cost, industrial production efficiency and economic impact, and spore production obstruction.

Active Publication Date: 2021-02-09
中国烟草总公司海南省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that spores are resistant to high temperature and high pressure, drying, acid and alkali, and organic solvents. They play an important role in the fields of medicine and animal husbandry, and have very important research and application values. However, in industrial fermentation production, they are ubiquitous. Uncontrolled spore production, low spore rate, low spore number, high production cost, etc., have caused obstacles and difficulties to the production of spores, and have had a negative impact on industrial production efficiency and economy

Method used

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  • Bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew
  • Bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew
  • Bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Isolation and Identification of Suppressing Cigar Tobacco Mildew Bacterial Strain YC-9

[0066] 1. Sample and culture medium

[0067] Sample: Guangxi tobacco leaves.

[0068] Separation medium: LB medium (Luria-Bertani broth): 10.0g peptone, 5.0g yeast extract powder, 10.0g NaCl, 15.0g-20.0g agar powder, 1000mL distilled water, pH7.0. Sterilized at 115°C for 20 minute.

[0069] Cut the Guangxi tobacco leaf samples into pieces and mix them evenly. Take 1.0 g of the samples and place them in a conical flask (with glass beads) filled with 99 mL of sterile water, and vibrate on a shaker at 150 r / min for 30 min. 10-fold dilution method was used to prepare 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 For each dilution, draw 100 μL and place it on the LB solid medium, and use a coating rod to coat the surface. After coating, put the LB solid medium upside down in a constant temperature incubator at 30°C for 1-2 days. Determine the appropriate concentration, select a pl...

Embodiment 2

[0078] Embodiment 2, bacillus highlander YC-9 has mold inhibitory effect to tobacco

[0079] 1. Tablet antagonism test

[0080] Detect the antibacterial effect of Bacillus uplander YC-9 of Example 1 on fungi, the fungi to be tested are Trichoderma longibrachiatum (Trichoderma longibrachiatum) ACCC 32522, Aspergillus niger (Aspergillus niger) ACCC31566, Aspergillus nidulans (Aspergillus nidulans) ACCC 32571 .

[0081] Transfer Bacillus highlander YC-9 and the fungus to be tested to LB and PDA medium respectively for strain activation. After inoculation, place the petri dish in a constant temperature incubator and culture Bacillus highlander YC-9 at 30°C for 1 day. The test fungi were cultured at 28°C for 3 days. Then use a hole puncher with a diameter of 6mm to inoculate the bacterial block from the edge of the fungal colony to the center of the new PDA plate, and use the inoculation loop to pick the Bacillus highlander YC-9 colony and inoculate it at the midpoint of the four...

Embodiment 3

[0098] Embodiment 3, the characteristic of highland bacillus YC-9

[0099] This embodiment is by analyzing 71 kinds of carbon sources of the GenⅢ microplate of the Biolog system and the fully automatic growth curve on the growth amount, the growth cycle of Bacillus uplander YC-9 of embodiment 1, and the impact of producing spores, and determine it on the shake flask level The sporulation conditions of Bacillus highlander YC-9 were established. The results show that Bacillus highlander YC-9 has a better ability to metabolize carbon sources; the carbon sources with more spore production are L-malic acid and α-D-glucose; the optimal carbon source of Bacillus highterly YC-9 is inosine The spore rate is up to 60.47%.

[0100] 1 Materials and methods

[0101] 1.1 Materials

[0102] Test strain: Bacillus altitudinis YC-9.

[0103] Medium: LB liquid (solid) medium (g / L): yeast extract powder 5.0, peptone 10.0, sodium chloride 5.0, (agar 15-20), pH 7.0-7.2, the balance is water, st...

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Abstract

The invention discloses bacillus altitudinis and application thereof to prevention and control of cigar fermentation mildew. The bacillus altitudinis is bacillus altitudinis (bacillus altitudinis) YC-9, the preservation number of the bacillus altitudinis YC-9 in the General Microbiological Center of the Chinese Microbiological Culture Collection and Management Committee is CGMCC No.20884, the bacillus altitudinis can inhibit the growth of fungi and reduce the mildew of tobacco leaves, and the fermentation appearance quality of cigars can be improved by combining the bacillus altitudinis with bacillus velezensis (Bacillus velezensis) ACCC02735. It is shown that the bacillus altitudinis (bacillus altitudinis) YC-9 and the strain combination can be used for improving the quality of the tobacco leaves in the fermentation process.

Description

technical field [0001] The invention relates to a strain of bacillus uplander and its application in preventing and controlling cigar fermentation mildew in the field of biotechnology. Background technique [0002] There are many types and quantities of microorganisms in tobacco leaves, which can be basically divided into two types: beneficial microorganisms and harmful microorganisms. During the fermentation process of cigar leaves, through the co-catalysis of beneficial microorganisms, biological enzymes and chemical elements, the intrinsic aroma quality of tobacco leaves can be fully revealed and The use value of tobacco leaves can be improved, but fungal reproduction can reduce the utilization rate of tobacco leaves, destroy the physical properties of tobacco leaves, make tobacco leaves lose elasticity and be easily damaged, and produce musty smell, thus losing use value. [0003] Modulation and fermentation are the key links in the formation of cigar tobacco leaf qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22C12R2001/07C12N1/205
Inventor 顾金刚刘好宝杨明川马聪聪夏鑫鑫张磊罗泽华蔡斌辛玉华
Owner 中国烟草总公司海南省公司
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