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Efficient kitchen garbage degradation compound microbial agent as well as preparation method and application thereof

A technology of compound microbial bacterial agent and degrading bacteria, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of organic matter loss environment, low microbial activity, and ineffective function, so as to reduce the degradation cycle , improve the decomposing rate, and realize the effect of resource utilization

Pending Publication Date: 2022-06-03
JINHUA KOYON ENVIRONMENTAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, the use of microorganisms to degrade kitchen waste often has problems such as low microbial activity and ineffective performance, and some organic matter is converted into odorous substances containing hydrogen sulfide and ammonia nitrogen compounds, resulting in the loss of organic matter and environmental pollution.

Method used

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  • Efficient kitchen garbage degradation compound microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and identification of bacterial species

[0036] In the present embodiment, bacterial species separation and identification are carried out from the soil of Jinhua Shuanglongdong Scenic Spot and the cow dung heap of Jinhua Jiufeng Ranch, and the details are as follows:

[0037] (1) Isolation and identification of starch degrading bacteria

[0038] Take 0.5 g of soil sample or fecal sample, add 49.5 mL of distilled water, put it into a 250 mL conical flask with an appropriate amount of glass beads, and shake at 200 rpm for 1 h to obtain a sample solution.

[0039] After gradient dilution of the sample solution, 100 μL of the diluted solution was spread on starch agar medium. After culturing at 37 °C for 48 hours, an appropriate amount of iodine solution was added to the plate, and colonies with larger transparent circles were picked for purification and culture, and stored in -80 °C glycerol tubes. , and sent the obtained pure culture to Beijing Qing...

Embodiment 2

[0059] Embodiment 2 Bacterial species antagonism test

[0060] Bacillus velesi, Bacillus cereus, Bacillus highlandis, Bacillus subtilis and Bacillus amyloliquefaciens isolated and preserved in Example 1 were cultured in pairs on LB broth agar medium, and at the intersection point Observe the growth of bacteria. After testing, there is no mutual antagonism among the five kinds of bacteria on the surface of the medium, and it can be used for the preparation of the compound bacterial agent.

Embodiment 3

[0061] The preparation of embodiment 3 spore fermentation liquid

[0062] (1) slant culture: Bacillus velesi, Bacillus cereus, Bacillus highlandi, Bacillus subtilis and Bacillus amyloliquefaciens isolated and preserved in Example 1 were inoculated on the LB broth agar medium slant, respectively, 37 Activated by incubation for 24h.

[0063] (2) Seed liquid culture: inoculate Bacillus velesi, Bacillus cereus, Bacillus highlandi, Bacillus subtilis and Bacillus amyloliquefaciens activated in step (1) in LB broth liquid medium respectively, at 37°C , 200rpm shaker shaking culture for 24h, respectively, to obtain the seed liquid of the above-mentioned five kinds of bacteria.

[0064] (3) spore-producing fermentation culture: inoculate the seed liquids of Bacillus velesi, Bacillus cereus, Bacillus highlandis, Bacillus subtilis and Bacillus amyloliquefaciens obtained in step (2) respectively in an improved spore-producing fermentation medium (glucose 25g / L, soy peptone 15g / L, corn s...

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Abstract

The invention relates to an efficient kitchen waste degradation compound microbial agent and a preparation method and application thereof, the compound microbial agent comprises a degradation microbial agent and a deodorization microbial agent, and the degradation microbial agent comprises bacillus velezensis spores, bacillus cereus spores and bacillus altitudinis spores; the deodorizing microbial agent comprises bacillus subtilis spores and bacillus amyloliquefaciens spores. The strains are independently separated and preserved, and have excellent capability of degrading protein, fat, starch and cellulose, and the bacillus subtilis and the bacillus amyloliquefaciens also have the capability of efficiently removing NH3 and H2S. The composite microbial agent provided by the invention can quickly degrade the kitchen garbage, no odor is generated, the final product is a high-quality organic fertilizer, and harmless, reduction and resource utilization of the kitchen garbage is realized.

Description

technical field [0001] The invention relates to the technical field of microbial inoculants, in particular to a high-efficiency kitchen waste degrading composite microbial inoculum and a preparation method and application thereof. Background technique [0002] Kitchen waste refers to the kitchen waste and waste edible oils and fats generated in the daily life of residents in food processing, catering services, unit meals and other activities. , rich in nutrients and so on. [0003] Kitchen waste contains starch, dietary fiber, fat, protein and other organic substances, which are easily spoiled and deteriorated. Without proper treatment, it can easily lead to many serious environmental problems. At present, the treatment methods of kitchen waste mainly include: non-biological treatment methods (incineration, dehydration, vacuum frying and mechanical crushing), biological treatment methods (landfill, composting, anaerobic digestion). Among them, the non-biological treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F9/04B01D53/84C12R1/07C12R1/085C12R1/125
CPCC12N1/20C05F17/20C05F9/04B01D53/84Y02W30/40
Inventor 张康东潘盛淇李卓蕾林海峻
Owner JINHUA KOYON ENVIRONMENTAL TECH CO LTD
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