33results about How to "Phosphorus dissolving ability is strong" patented technology

The invention relates to a microbial agent and a preparation method as well as an application of the microbial agent. The microbial agent comprises functional bacteria and an adsorbent and is characterized in that the functional bacteria comprise bacillus subtilis, bacillus megatherium, trichoderma longibrachiatum and aspergillus niger, wherein the mass ratio of the microbial fermentations of the bacillus subtilis, the bacillus megatherium, the trichoderma longibrachiatum and the aspergillus niger in a mixture is 0.5-2:0.5-2:0.5-2:0.5-2. The microbial agent is prepared by carrying out the processes of slant culture, shake-flask culture, seeding tank fermentation, fermentation tank fermentation, microbial fermentation mixing, absorbent absorption, and low-temperature drying, and the microbial agent can be applied to the production of straw manure.

The invention provides new burkholderia cenocepacia and application thereof. The bacterium is identified as new burkholderia cenocepacia and is named as Burkholderia cenocepacia CJ-P8 with the collection number of CGMCC NO.12119. The new Burkholderia cenocepacia CJ-P8 disclosed by the invention is obtained by screening from radix pseudostellariae rhizosphere soil by adopting a plate confrontation process, respectively has a strong antagonistic effect on radix pseudostellariae soil-borne pathogens such as fusarium oxysporum and fusarium moniliforme which are obtained through separation in an early stage, and has no pathogenicity on other crops in a radix pseudostellariae producing area. The invention also provides an optimal medium for growth of the antagonistic bacterium, an optimal formula is 1 / 4 LB+1 / 80 MS (does not contain ferric salt)+0.1wt% of brown sugar, and fermented liquid has an obvious antagonistic effect. The antagonistic strain provided by the invention can be used for effectively preventing and treating soil-borne diseases of radix pseudostellariae and is pollution-free, ecological and safe, thereby having good application prospects.

The invention relates to bacillus subtilis HG-15 with salt resistance, disease prevention and growth promotion and inoculant preparation and application thereof. The HG-15 strain has high salt resistance and is capable of stabilizing colonization in saline alkali soil; the HG-15 strain can survive in liquid water-soluble fertilizer and solid water-soluble fertilizer and maintain a high survival rate, and the HG-15 strain, can be mixed with organic fertilizer, compound fertilizer, water-soluble fertilizer, controlled-release fertilizer and the like for use; the strain has a wide antibacterial spectrum and has a good control effect on root rot diseases of crops such as wheat, corn, vegetables and fruit trees and other soil-borne diseases; the contents of available phosphorus and available potassium in soil can be significantly improved, and thus the application amounts of phosphorus chemical fertilizer and potassium chemical fertilizer are reduced; the strain has excellent fermentation characters, and an inoculant preparation technology is simple and low in cost, and industrial production and transportation are facilitated.

The invention provides a phosphate dissolving bacterium and an acquisition method thereof, belonging to the field of agricultural biotechnology. The phosphate dissolving bacterium and the acquisition method thereof can enrich the genetic resource of wild phosphate dissolving bacteria and enlarge a backup library for whole-genome breeding of phosphate dissolving bacteria. The phosphate dissolving bacterium provided by the invention is named as YYF22 and is preserved in China Center for Type Culture Collection with an accession number of CCTCC No: M2015043. The colony characteristics of the phosphate dissolving bacterium are as follows: the YYF22 strain is coccobacillus with a length of 1.2 to 2.9 [mu]m and a width of 0.4 to 1.5 [mu]m; the phosphate dissolving bacterium is normally arrayed in pairs and may independently exist; the phosphate dissolving bacteria sometimes adhere to from a filose shape or chain shape; the phosphate dissolving bacterium is free of flagellum, has a capsule, and cannot move; and on an LB flat, the colony of the bacterium has a neat edge, a moist surface and low bulges, and is glossy and cream yellow.

The invention provides a compound alga oligosaccharide microbial fertilizer and a preparation method thereof and relates to a microbial fertilizer. The compound alga oligosaccharide microbial fertilizer is prepared from, by volume, an alga oligosaccharide solution 20%-80% and bacillus mucilaginosus fermented liquid 20%-80%, wherein the effective number of living bacteria is greater than or equal to 0.2 billion / mL, the alga oligosaccharide content is greater than or equal to 0.5 g / kg, and a pH value is 6.8-7.2. The preparation method comprises the steps that bacillus mucilaginosus is activated by using a solid nitrogen-free culture medium and then is inoculated to a seed culture medium to obtain primary seed fermentation liquid; the primary seed fermentation liquid is inoculated to a seed fermentation tank, and culture is performed to obtain secondary seed liquid; the second seed liquid is inoculated to a fermentation culture medium, and fermentation culture continues to prepare the bacillus mucilaginosus fermented liquid; the alga oligosaccharide solution and the bacillus mucilaginosus fermented liquid are mixed to obtain the compound alga oligosaccharide microbial fertilizer. The compound alga oligosaccharide microbial fertilizer has the effects of improving soil, improving fertilizer efficiency, adjusting crop growth, improving crop quality and the like.

The invention discloses enterobacter ludwigii PN6 and application thereof. The classification name of the strain is Enterobacter ludwigii with a strain number of PN6, the strain is collected in the China Center for Type Culture Collection and has a collection number of CCTCC NO: M 2019881. The strain PN6 screened from a Phyllostachys edulis root system has an obvious anti-bacteria function for a pathogenic bacterium, i.e., C .phyllostachydis, of the shoot blight of Phyllostachys edulis, a fermentation liquor inhibition rate of the strain PN6 can be 49.28%, and in addition, the strain PN6 has acapacity of efficiently dissolving phosphorus and secreting IAA (indole-3-acetic acid). The strain PN6 disclosed by the invention has an acceleration function for the growth of Phyllostachys edulis,an experiment data displays that a liquid fungicide prepared from the PN6 has an obvious growth promotion function for growth indexes, including the ground diameter, the seedling height, the chlorophyll and the like of the Phyllostachys edulis, in addition, the stress resistance of the Phyllostachys edulis can be increased, and an occurrence probability of the shoot blight of Phyllostachys edulisis reduced. Therefore, the invention provides an excellent strain resource for developing an environmentally-friendly special biofertilizer for the Phyllostachys edulis.

The invention provides a Korean pseudomonas and application thereof. The Korean pseudomonas is identified as Korean pseudomonas CJ-361, and the preservation number is CGMCC NO.12122. The Korean pseudomonas CJ-361 is obtained through separation from radix pseudostellariae rhizosphere soil by adopting a pseudomonas selectivity culture medium, an antagonistic experiment is performed on fusarium oxysporum and fusarium moniliforme separated to the radix pseudostellariae root lesion portion through an agar plate opposite culture method, and it is found that the Korean pseudomonas has the strong antagonism on the fusarium oxysporum and the fusarium moniliforme. Meanwhile, the invention provides the optimum culture medium for antagonistic bacterium growth, the optimum formula is 1 / 4LB+1 / 20MS (containing no ferric salt)+0.1 wt% brown sugar, and the fermented liquor has the obvious antagonistic effect. The antagonistic strain and the microbial agent thereof can effectively prevent and treat radix pseudostellariae root rot diseases, and the environment-friendly, ecological and safe biological prevention and control potential strain is provided for overcoming or relieving replantation diseases of radix pseudostellariae.

The invention provides a preparation method of modified biochar, the modified biochar, a composite material and a soil repairing agent, and relates to the technical field of biochar. The preparation method of the biochar comprises the following steps: mixing peanut shell powder with a graphene oxide suspension to obtain peanut shell powder impregnated with the graphene oxide suspension; mixing theobtained peanut shell powder impregnated with the graphene oxide suspension with the nano-SiO2 to obtain a mixed material; mixing the obtained mixed material with water to obtain a mixture; carbonizing the mixture to obtain hydrated carbon, and drying the obtained hydrated carbon to obtain the modified biochar. The modified biochar provided by the invention has porosity, large specific surface area and good adsorption capacity, and can effectively reduce the activity of soil heavy metals in the soil.

The invention discloses a phosphate solubilizing bacterium and an extraction method thereof. The phosphate solubilizing bacterium is named a strain B5, the strain B5 is a pseudomonas bacterium, determination results of biochemical criterion are gram staining is negative, a methyl red test is negative, a V-P reaction is negative, an indole test is negative, an oxidase test is negative, the strain B5 can use citrate as a carbon source to produce extracellular enzyme-gelatinase liquidation gelatin, catalase and indole are produced, and H2S is generated; the order of the phosphate solubilizing ability of the strain B5 from high to low under different carbon sources is that glucose is greater than galactose, the galactose is greater than lactose, the lactose is greater than sucrose, and the sucrose is greater than soluble phosphate starch, and the order of the phosphate solubilizing ability under different nitrogen sources is that ammonium sulfate is greater than ammonium chloride, the ammonium chloride is greater than urea, and the urea is greater than potassium nitrate; and the optimum growth temperature of the strain B5 is 28-32 DEG C, and the optimum growth pH of the strain B5 is 7-9.

The invention relates to microbial fertilizer specially used for casuarinas. The invention is characterized in that the invention comprises liquid bacterial fertilizer which is obtained by mixing the fermentation broth of a Bacillus megaterium de Bary (Bacillus megaterium de Bary), a fermentation broth of a Frankia.sp (Frankia.sp) and stable effect promoter in a volume ratio of 1 to 1.5: 1.5 to 2.0: 0.1-0.5. The components of the stable effect promoter are counted by a mass fraction: 0.01 to 0.05 percent of flavonoids, 0.001 to 0.01 percent of coumarin, 0.001 to 0.01 percent of sodium molybdate, 0.001 to 0.01 percent of cobalt sulfate; the rest is water. The stable effect promoter also comprises a zeolite powder and organic fertilizer. The fertilizer can promote the growth of the casuarinas and increase the survival ratio and preservation ratio of the casuarinas as well as increasing the quantity of the casuarinas seedling with a nodule. Therefore, the resisting performance and the drought-resistance ability of the casuarinas are promoted. The crown closure speed of the plantation is increased. The windbreak and sand fixation effect of the casuarinas in the coastal protection forest is strengthened. The strain CCTCC M 207197 of Frankia.sp fgc6 provided by the invention has a better effect.

The invention discloses bacillus altitudinis having a phosphate-solubilizing function and application thereof. The bacillus altitudinis is preserved in China Center for microbial culture collection management committee general microbiology on 18th, March, 2019, of which the preservation number is CGMCC No.17338. The bacillus altitudinis preferably adopts an LB liquid culture medium used in culturing of laboratory liquid and a Mongina inorganic phosphate solid culture medium used in phosphate-solubilizing bacteria. According to the bacillus altitudinis having a phosphate-solubilizing function and application thereof provided by the invention, an experiment proves that effective phosphate concentration in blank control solutions of uninoculated bacteria is much less than that in fermented liquid of bacillus altitudinis SM01, that is, the bacillus altitudinis SM01 is an efficient phosphate-solubilizing fungus. A strain of the bacillus altitudinis disclosed by the invention has the characteristics of being high in growth speed, high in phosphate-solubilizing capability, high in stress resistance, and high in capability of quickly and massively colonizing on rhizosphere of plants, and has a good application prospect.

The invention discloses Gibberella intermedia and an application thereof, and belongs to the technical field of phosphate-dissolving microorganism fertilizers. The strain is collected in China General Microbiological Culture Collection Center on December 9th, 2016, and the collection number is CGMCC No. 13199. The invention further discloses a microbial agent comprising the strain and a preparation method of the microbial agent. Experiments indicate that the strain has the advantages of high growth speed and strong phosphate-dissolving capacity. The microbial agent prepared from the strain can increase phosphorous fertilizer utilization rate and improve soil structure, and further crop yield increasing effects are achieved.

The invention discloses pantoea dispersa and an application thereof in preparation of a microbial fertilizer, wherein the pantoea dispersa is classified and named as pantoea dispersa, is preserved inChina General Microbiological Culture Collection Center, has the preservation number of CGMCC No.14876, and is preserved on November 14th, 2017; and the pantoea dispersa is specifically applied for seed blending in preparation of the microbial fertilizer. The pantoea dispersa has the beneficial effects: (1) the pantoea dispersa has fast growth rate and strong phosphorus removal ability, and not only can dissolve insoluble organophosphorus but also can dissolve insoluble inorganic phosphorus; (2) the pantoea dispersa has strong stress resistance and can grow in an LB culture medium with the salinity of 6%; (3) the pantoea dispersa can promote development of plant root systems and promote growth of plants after being mixed with corn seeds; and (4) the pantoea dispersa used in cultivated landcan improve the utilization rate of soil phosphorus elements, reduces the use of chemical fertilizers, and avoids the problem of soil structure.

The invention discloses phosphate solubilizing bacteria PC05 and an isolating and screening method thereof. The strain name of the phosphate solubilizing bacteria PC05 is Inquilinus ginsengisoli PC05. The phosphate solubilizing bacteria PC05 are preserved in China Center for Type Culture Collection on June 24, 2016 in Wuhan University which is in Wuhan, China, with the preservation number of CCTCC NO:M2016347. The cultivated phosphate solubilizing bacteria PC05 can enrich wild phosphate solubilizing bacteria genetic resources, have high phosphate solubilizing capacity, and can increase the content of available phosphorus in soil, increase the utilization efficiency of insoluble phosphorus in soil, and reduce the application amount of phosphatic fertilizer.

The invention discloses a low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium as well as a culture method and application thereof.The bacterium is separated from saline-alkaline soil in small Kongtong cave ditches in Qingyang City, Gansu Province, identified as Acinetobacter calcoaceticus and named as Acinetobacter calcoaceticus QY-3, the preservation unit of the bacterium is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Wuhan, China, the preservation date is 2021.1. 14, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2021067. The low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacteria provided by the invention can tolerate saline-alkaline environments with pH values of 8 and 50 g / L NaCl, pH values of 9 and 30 g / L NaCl and pH values of 10 and 10 g / L NaCl to the maximum extent under a shaking table culture condition of 15 DEG C, and insoluble Ca3 (P04) 2 can be effectively converted into soluble phosphorus. The low-temperature-resistant saline-alkaline-tolerant phosphate solubilizing bacterium provided by the invention is high in stress resistance and has relatively high phosphate solubilizing capacity under low-temperature saline-alkaline conditions, so that the use of phosphate fertilizer in saline-alkaline land is reduced, and a solution is provided for saline-alkaline land treatment in China.

The invention provides aspergillus niger with dual-function of phosphorus-dissolving and bio-control. The aspergillus niger is preserved in the China Center for Type Culture Collection (CCTCC) on August 25, 2016; the preservation number is CCTCC NO: M2016427; the classification name is aspergillus niger HY30. The viable bacterium amount of the aspergillus niger in a microorganism preparation containing the strain is 10<8>CFU / g at least; the aspergillus niger has a phosphorus-dissolving function and also has the effect of preventing and treating soil-borne diseases.

The invention discloses eupenicillium cinnamopurpureum strain and an application thereof, and belongs to the technical field of P-solubilizing microbial fertilizers. The bacterial strain is eupenicillium cinnamopurpureum WHP-1, and the preservation number is CGMCC No. 8128. The invention also discloses a microbial inoculum containing the bacterial strain and a preparation method thereof. Experiments show that the eupenicillium cinnamopurpureum strain WHP-1 has the characteristics of fast growth speed, large sporulation quantity, strong stress resistance, strong P-solubilizing performance and the like, and can be fast colonized in quantity at the plant roots. The fermentation broth or the microbial inoculum of the bacterial strain contains a large amount of P-solubilizing bacterium, and has certain active effect on soil improvement; and due to the P-solubilizing function, the fermentation broth or the microbial inoculums has active influence on improvement of soil structure, enhancement of fertilizer absorptivity of crops, and the like.

The invention discloses a method for preparing microbiological micro-ecological inoculum by using corn steep liquor as raw materials through a symbiosis fermentation technique, and relates to the fields of biological technology and microecology, and mainly solves the problems to be difficult to treat, that the corn steep liquor is high in acidity, toxins easily exceed the standard, and the added value is low. The method can promote resource reutilization of corn steep liquor waste. The microbiological micro-ecological inoculum is liquid microbiological micro-ecological inoculum. The preparation steps mainly adopt the components of 10-30 parts of corn steep liquor, 1-4 parts of corn flour, 0.1-0.5 part of magnesium sulphate, 1-2 parts of potassium hydroxide and the balance water. Under thecondition that inoculum concentration of bacillus subtilis is 1-5%, the inoculum concentration of bacillus licheniformis is 2-8%, the inoculum concentration of aspergillus niger is 2-8%, the fermentation temperature is 28-35 DEG C, the rotating speed is 120-180rpm, and the liquid loading amount is 1 / 2 to 1 / 5, fermentation is performed for 2-3 days. A potassium hydroxide neutralizing scheme is adopted, and the symbiosis fermentation technique is utilized, so that a high activity multi-bacteria microbiological system is realized, and finally, high cell amount and effects of removing phosphorus and potassium can be obtained. According to the technique, the added value of core processed by-products can be increased, besides, the obtained microbiological micro-ecological inoculum product can beused for preparing a biological organic fertilizer, and crop growth is promoted.

The invention provides a root nodule endophyte S43 with a phosphate solubilizing function and application of the root nodule endophyte S43. A root nodule endophyte pseudomonas koreensis S43 strain is obtained through separation, the strain is preserved in Guangdong Microbial Culture Collection Center on December 8, 2021, and the preservation number is GDMCC No: 62100. Researches show that the strain has strong phosphate solubilizing ability, the content of soluble phosphorus in supernate of the strain after fermentation reaches 400 mu g / ml, and the content of the soluble phosphorus is increased along with the prolonging of fermentation time; in addition, the strain S43 also has hydrogen secreting capacity, can reduce the pH value of a culture medium from 7.0 to 4.13, is beneficial to converting insoluble phosphorus into soluble phosphorus which can be absorbed and utilized by plants, and promotes absorption and utilization of crops. According to the invention, a new degrading bacterium selection is provided for a soil phosphorus-dissolving microbial strain, a strain resource library of phosphorus-dissolving bacteria is expanded, and absorption and utilization of phosphorus by plants and improvement of soil fertility are facilitated.

The invention discloses a Bacillus horneckae and application thereof and belongs to the technical field of phosphate solubilizing microbial fertilizers. The strain has been already preserved in CGMCC, with the preservation data of 9th, January, 2017 and the preservation number of CGMCC No. 13551. The invention also discloses a microbial agent containing the strain and a preparation method thereof. Experiments verify that the strain has the characteristics of being high in growing speed, strong in phosphate solubilizing ability, fast to plant at plant rhizospheres and the like. The microbial agent prepared by the strain has the phosphate solubilizing function and can improve the soil structure, so that the utilization rate of a phosphate fertilizer is favorably increased, and therefore, the microbial agent is of great significance in improving the output and quality of crops.

The invention relates to microbial fertilizer specially used for casuarinas. The invention is characterized in that the invention comprises liquid bacterial fertilizer which is obtained by mixing the fermentation broth of a Bacillus megaterium de Bary (Bacillus megaterium de Bary), a fermentation broth of a Frankia.sp (Frankia.sp) and stable effect promoter in a volume ratio of 1 to 1.5: 1.5 to 2.0: 0.1-0.5. The components of the stable effect promoter are counted by a mass fraction: 0.01 to 0.05 percent of flavonoids, 0.001 to 0.01 percent of coumarin, 0.001 to 0.01 percent of sodium molybdate, 0.001 to 0.01 percent of cobalt sulfate; the rest is water. The stable effect promoter also comprises a zeolite powder and organic fertilizer. The fertilizer can promote the growth of the casuarinas and increase the survival ratio and preservation ratio of the casuarinas as well as increasing the quantity of the casuarinas seedling with a nodule. Therefore, the resisting performance and the drought-resistance ability of the casuarinas are promoted. The crown closure speed of the plantation is increased. The windbreak and sand fixation effect of the casuarinas in the coastal protection forest is strengthened. The strain CCTCC M 207197 of Frankia.sp fgc6 provided by the invention has a better effect.

Provided is an application of a cured inorganic acid in fertilizer production and fertilization; an inorganic acid is cured into a semi-solid or solid material by a chemical curing method, and the semi-solid or solid material is used as a solid phosphate-solubilizing agent and used for dry-method production of a high-concentration nitrogen and phosphorus compound fertilizer directly from a phosphate ore (powder) through one-time processing at room temperature, sylvite, cement kiln dust or some potassium ores, humic acid substances or trace element compounds and the like also can be added for together preparing a compound fertilizer with multiple nutrients; ammonia also can be used for acting with the cured acid to prepare a new nitrogen-containing fertilizer through a dry method or a semi-dry method. The method for producing the fertilizer with the cured acid has the advantages of simple process, easy operation, low energy consumption, no three wastes, no strict requirement on the grade of ores and the like and is suitable for general promotion. In addition, the cured acid also can be used as an additive and is applied with other fertilizers (trace element fertilizers or various phosphate fertilizers soluble in citric acid and the like) together, and the fertilizer efficiency of other fertilizers can be improved.

The invention discloses a strain of Raoultella ornithinolyticum and its application. The invention discloses a strain of Raoultella ornithinolytica, the preservation number of which is CGMCC No.9407. The Raoultella ornithinolytica WN‑3 disclosed by the present invention with a preservation number of CGMCC No.9407 is tolerant to an oligotrophic living environment and has good phosphorus-solubilizing ability, which can promote the improvement of soil phosphorus utilization rate. Raising, increasing crop yield and / or improving its traits, improving farmland soil, has significant economic, environmental and social benefits.