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A sedum rhizosphere-promoting Pseudomonas aeruginosa and its application

A technology for promoting growth of Pseudomonas aeruginosa and rhizosphere is applied in the field of plant microorganisms to achieve the effects of promoting growth, increasing germination number and crown width, and increasing molding rate

Active Publication Date: 2019-01-25
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are research reports about inoculating plant rhizosphere growth-promoting bacteria to improve the survival rate and seedling quality of twig cuttings, but there are few research reports on the screening of Sedum PGPR strains and their application in Sedum seedling cultivation

Method used

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  • A sedum rhizosphere-promoting Pseudomonas aeruginosa and its application
  • A sedum rhizosphere-promoting Pseudomonas aeruginosa and its application
  • A sedum rhizosphere-promoting Pseudomonas aeruginosa and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening and identification of bacterial strain JWP7

[0023] The screening and isolation process of Pseudomonas aeruginosa (Pseudomonas aeruginosa) JWP7 is as follows:

[0024] (1) Collect 12 soil samples from the rhizosphere of the sedum succulent plant in the sedum succulent plant production base of Huanglou Town, Qingzhou City, Shandong Province, weigh 10g of the soil sample respectively, add 90ml sterile water, 37°C, 180r / min, and cultured on a shaking table for 30 min. After gradient dilution, 0.1ml of the bacterial solution was applied to LB medium and Gao's No. 1 medium respectively, and a single colony was obtained after culture.

[0025] (2) pick single bacterial colony three zone lines to purify bacterial classification, in organophosphorus medium (glucose 10.0g; (NH4) 2 SO 4 0.5g; MgSO 4 ·7H 2 O 0.3g; MnSO 4 4H 2 O 0.03g; KCl 0.3g; FeSO 4 ·7H 2 O 0.03g; NaCl 0.3gCaCO 3 5.0g; Lecithin 0.2g; Distilled water 1000ml; pH7.0-7.5) and inorgani...

Embodiment 2

[0034] Example 2 JWP7 growth-promoting sedum growth test

[0035] (1) Preparation of JWP7 bacterial agent

[0036] Activate the JWP7 stored on the slant by three-section line method in LB (peptone 10g; NaCl 10g; yeast extract 5g; agar 15-20g; water 1000ml; pH 7.0-7.2, aliquot and sterilize at 121°C for 20min ) on the tablet. Pick a single colony into the corresponding 10ml liquid medium, and culture at 37°C and 180rpm for 24h. The seed solution was inoculated into corresponding 50ml liquid medium for fermentation according to 1% inoculum amount, and cultured at 37°C and 180rpm for 2 days to obtain JWP7 bacterial agent.

[0037] (2) Take Sedum sedum as the experimental material, the cutting substrate is peat and perlite with a mass ratio of 3:1, select Sedum sedum leaves of the same size, and remove them from the plant and place them in a ventilated and cool place for 10 days , Insert the leaves into the plug substrate at an angle of 45° to a depth of 1 cm, and cut 50 leaves...

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Abstract

The invention provides a sedum rhizosphere growth-promoting Pseudomonas aeruginosa (Pseudomonas aeruginosa) JWP7, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 7, 2016, and the preservation number is CGMCC No. .11969. The strain has good phosphorus-solubilizing ability, can successfully colonize the rhizosphere soil of sedum succulent plants, and can significantly promote the growth of roots and seedlings of sedum succulent plant cuttings, and increase the number of germination and crown of sedum. It can improve the molding rate of Crassulaceae plants, and the strain has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of plant microorganisms, and in particular relates to a sedum rhizosphere growth-promoting Pseudomonas aeruginosa and its application. Background technique [0002] Crassulaceae is a family of dicotyledonous plants. The plants of this family are perennial succulent herbs that bloom in summer and autumn. The flowers are small and luxuriant. The epidermis has waxy powder and the stomata are sunken to reduce transpiration. It is a typical xerophyte with strong asexual reproduction. The sedum plants are short, and because they are fleshy, they consume little water and fertilizer, so they are very easy to grow and watch. In recent years, the market demand for sedum succulents has grown rapidly. The annual market demand for succulents used only for interior decoration has exceeded 100 million pots, and the annual output value has exceeded 500 million yuan. [0003] Leaf cutting propagation is currently the main ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/385
CPCA01N63/10C12N1/20C12N1/205C12R2001/385
Inventor 刘方春马海林丁延芹杜秉海马丙尧杜振宇姚良同李丽单连娟
Owner SHANDONG FOREST SCI RES INST
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