1835 results about "Pseudomonas aeruginosa" patented technology

The invention provides isolated polypeptide and nucleic acid sequences derived from Pseudomonas aeruginosa that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

The invention relates to a composition for driving and killing mosquitoes. The composition is characterized by comprising the following substances by portions: 20 to 50 portions of natural essential oil, 10 to 35 portions of pyrethroid, 25 to 50 portions of solvent oil, 3 to 20 portions of surface active agent and 5 to 15 portions of antioxidant. The composition has the advantages of: (1) having no toxic side effect to body, having multiple action of driving mosquitoes, sterilizing and essence, being capable of reducing the cost of the product and bringing pleasant feeling to a user; (2) having different effective components with different action mechanism for target insects and also being capable of effectively retarding the drug resistance thereof; (3) having higher safety than chemosynthetic insecticide due to easy decomposition and low residue of the natural essential oil in natural environment; (4) having obvious control effect to mosquitoes, flies and cockroaches and being capable of effectively reducing the using amount of pyrethroid due to adding of the natural essential oil; and (5) having a certain action of restraining and killing various harmful bacterium such as golden staphylococcus aureus, shigella shigae and Pseudomonas aeruginosa due to adding of natural vegetable garlic oil.

It has been discovered that fluids viscosified with viscoelastic surfactants (VESs) may have their viscosities reduced (gels broken) by the direct or indirect action of a biochemical agent, such as bacteria, fungi, and / or enzymes. The biochemical agent may directly attack the VES itself, or some other component in the fluid that produces a by-product that then causes viscosity reduction. The biochemical agent may disaggregate or otherwise attack the micellar structure of the VES-gelled fluid. The biochemical agent may produce an enzyme that reduces viscosity by one of these mechanisms. A single biochemical agent may operate simultaneously by two different mechanisms, such as by degrading the VES directly, as well as another component, such as a glycol, the latter mechanism in turn producing a by-product (e.g. an alcohol) that causes viscosity reduction. Alternatively, two or more different biochemical agents may be used simultaneously. In a specific, non-limiting instance, a brine fluid gelled with an amine oxide surfactant can have its viscosity broken with bacteria such as Enterobacter cloacae, Pseudomonas fluorescens, Pseudomonas aeruginosa, and the like.

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effuses L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 mn in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

The present invention describes a method for treating, removing or preventing a bacterial infection, which method comprises administering to a human suffering, suspected of suffering or at risk of suffering from Staphylococcus aureus (S. aureus) infection, a Streptococcus infection, Escherichia coli (E. coli) infection, Pseudomonas aeruginosa (P. aeruginosa) infection, Acinetobacter baumannii (A. baumannii) infection, Enterococcus faecium (E. faecium) infection and / or Clostridium difficile (C. difficile) infection, an effective amount of human polyclonal antibodies affinity purified from a human blood sample with an antigenic preparation comprising cellular and / or secreted antigen(s) from bacterial cells selected from S. aureus, a Streptococcus, E. coli, P. aeruginosa, A. baumannii, E. faecium, C. difficile or a combination thereof, and optionally, wherein said affinity purified human polyclonal antibodies are purified (e.g., as made more concentrated as compared to the starting or unpurified material) relative to the same human polyclonal antibodies in the unpurified or non-affinity-purified human blood sample, e.g., intravenous immunoglobulin (IVIG) sample, and / or also optionally, wherein said affinity purified human polyclonal antibodies are specific for the bacterial antigens used in the affinity purification, and / or further optionally wherein the affinity purified human polyclonal antibodies are substantially free of human antibodies that specifically bind to non-bacterial antigens in the human blood sample. Pharmaceutical compositions for treating bacterial infections, comprising an effective amount of human polyclonal antibodies affinity purified from a human blood sample with an antigenic preparation comprising cellular and / or secreted antigen(s) from S. aureus, Streptococcus, E. coli, P. aeruginosa, A. baumannii, E. faecium, C. difficile or a combination thereof, are also provided.

Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

The invention relates to a compound microbial agent which comprises compound bacteria for decomposing kitchen wastes and auxiliary materials for solid fermentation, wherein the compound bacteria comprise 10-15 parts of cellulose degradation bacteria, 15-30 parts of starch degradation bacteria, 15-20 parts of protein degradation bacteria, 15-20 parts of grease degradation bacteria, 10-15 parts of bacillus subtilis and 5-15 parts of pseudomonas aeruginosa; the auxiliary materials comprise 50-60 kg of wheat bran, 15-20 kg of bean pulp, 0.4-0.6 kg of magnesium sulfate, 0.4-0.6 kg of potassium dihydrogen phosphate, 0.1-0.2 kg of disodium hydrogen phosphate and 8-10 kg of glucose. The inoculation amount of the compound bacteria in the auxiliary materials is 0.8%-1%, and the compound bacteria is subjected to solid fermentation to obtain the compound microbial agent. The compound microbial agent has a good decomposing effect on greases, proteins and fiber substances and can be tolerant to salt with a certain concentration; optimally, the degradation rate can reach more than 90%.

The invention provides isolated polypeptide and nucleic acid sequences derived from Pseudomonas aeruginosa that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

The invention relates of administration of an attenuated Salmonella strain expressing a lipopolysaccharide O antigen from a suitable pathogen, in particular Pseudomonas aeruginosa, and the use of the same as a vaccine to promote sterile immunity to the pathogen, e.g., P. aeruginosa, via intranasal vaccination. In one embodiment, the present invention is directed to a unique intranasal route of immunization for the delivery of relevant heterologous polysaccharide antigens via a live, attenuated Salmonella strain.

The invention discloses a complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and a restoring method thereof. The microbial body of the complex microbial inoculant comprises Pseudomonas aeruginosa, Pseudomonas fulva, Acinetobacter lwoffii, Nocardioides luteus and Streptomyces flavoviridis. The invention provides a method for restoring petroleum-contaminated soil in loess highlands by using the complex microbial inoculant, which comprises the following steps of (1) carrying out pretreatment on contaminated soil; (2) degrading the contaminated soil; (3) covering a plastic film on the soil; and (4) adding water, urea, diammonium phosphate and the complex microbial inoculant. By using the method disclosed by the invention, the process is simple, the cost is low, and the degradation effect is significantly increased.

Described are synergistic antimicrobial-fragrance compositions including broad spectrum antimicrobial compositions containing salicylaldehyde and at least one organoleptically-compatible antimicrobial synergism cofactor substance. The weight ratio range of salicylaldehyde:synergism cofactors substance is from 1:10 up to 10:1. The cofactor substance is such that the degree of synergism of the resultant mixture is defined according to the IFF Antimicrobial Synergism Test wherein the difference between the actual and expected antimicrobial values of the mixture is greater than or equal to a multiple of (i) 0.05 and (ii) the expected antimicrobial value of the mixture. Cofactor substances include phenolics such as cresol, caravacrol and thymol; ethyl vanillin; benzyl alcohol; indol; beta-orcinol; and terpinenol-4. Microorganisms against which the synergistic compositions are effective include:Escherichia coli;Enterococcus hirae;Pseudomonas aeruginosa;Staphylococcus aureus; andSaccharomyces cerevisae.The compositions have application in all-purpose cleaning compositions, gel-type toilet rim articles, liquid-type toilet rim articles, personal shower cleaning compositions, and body and hair care products including shower gel compositions, shampoo compositions and foam bath compositions.

The present invention relates to cupredoxin, specifically Pseudomonas aeruginosa azurin, and / or Pseudomonas aeruginosa cytochrome C551 and their use in inhibiting of viral infection, and in particular infection of mammalian cells by the Human Immunodeficiency Virus (HIV). The invention also relates to variants and derivatives of cupredoxin and cytochrome c that retain the ability to inhibit viral infection, and in particular infection by the Human Immunodeficiency Virus (HIV). The invention also relates to research methods for studying viral and bacterial infection in mammalian cells.

The invention relates to an andrographolide derivative, which has the structure as shown in a general formula I: wherein, R1, R2 and R3 are the same or different hydrogen, substituted or non-substituted organic acid radical, inorganic acid radical, alkyl, aryl or heteroaryl, while at least one of R1, R2 and R3 is R-lipoic acid or S-lipoic acid or the mixture of R-lipoic acid and S-lipoic acid or the corresponding dihydrolipoic acid or acetylcysteine radical of R-lipoic acid or S-lipoic acid; the derivative has good anti-tumor effect, and the derivative can cause apoptosis of tumor cells, directly eliminate gram positive bacteria, staphylococcus aureus and sensitivities MRSA5676 and MRSA5677, inhibit the QS system of gram negative bacteria and pseudomonas aeruginosa and inhibit and damage the formation of the bio-film of pseudomonas aeruginosa; the product has prominent hypoplycemic effect and is suitable for the preparation of the medicines that can cure cancers, inflammations, diabetes mellitus and bacterial and viral infection.

The invention relates to nano-silver solution with the nano-silver concentration between 250 and 3,200ppm and the average sliver grain diameter between 2 and 12nm. The nano-silver solution also comprises medicine accessory level dispersing agents and medicine level reducing agents. The preparation method mainly comprises the following steps of: dissolving the medicine accessory level dispersing agents into deionized water to prepare dispersing agent solution; adding and dissolving silver nitrate to obtain solution I; taking and adding the medicine accessory level reducing agent solution into the solution I; mixing the medicine accessory level reducing agent solution with the solution I to obtain solution II; placing the solution II under an ultraviolet lamp to be irradiated; and stirring the solution II for reaction to obtain the nano-silver solution. The nano-silver solution has stronger antibiosis performance. When the nano-silver solution is diluted to 10ppm to detect and evaluate the antibiosis effect on staphylococcus aureus, colibacillus and pseudomonas aeruginosa, the antibiosis efficiency reaches higher than 99.9 percent in two minutes. The preparation method has simple process, can avoid the use of various strong acid, strong base and strong reducing agents and has good medical safety.

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg / kg to 1257 mg / kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

A formulation comprising molecular arrangements capable of penetrating pores in a barrier, owing to penetrant adaptability, despite the fact that the average diameter of said pores is smaller than the average penetrant diameter, provided that the prenetrants can transport agents or else enable agent permeation through the pores after penetrants have entered pores, characterized in that the formulation comprises at least one consistency builder in an amount that increases the formulation to maximally 5 Nm / s so that spreading over, and retention at, the application area is enabled and / or at least one antioxidant in an amount that reduces the increase of oxidation index to less than 100% per 6 months and / or at least one microbiocide in an amount that reduces the bacterial count of 1 million germs added per g of total mass of the formulation to less than 100 in the case of aerobic bacteria, to less than 10 in the case of entero-bacteria, and to less than 1 in the case of Pseudomonas aeruginosa or Staphilococcus aureus, after a period of 4 days.

The invention comprises a substrate for detection of micro-organisms, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeieties, to a di-, or tripeptide consisting of amino acids X1 and X2, or X1, X2 and X3, in which one of them, for example X1, is a D-amino acid and the others, for example X2 and X3, may be any D- or L-amino acid. Said substrate preferably is used for the detection of Bacillus anthracis. Alternatively, the invention is directed to a substrate for detection of micro-organisms, more specifically P. aeruginosa, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeities to a tri- tetra or pentapeptide consisting of glycine amino acids. The invention further comprises methods for detection of micro-organisms, specifically Bacillus anthracis and Pseudomonas aeruginosa, with the substrates of the invention and use of the substrate(s) in such a method.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention provides a cosmetic containing a plant bacteriostatic composition, containing 0.2-2.0% by weight of plant bacteriostatic composition; the plant bacteriostatic composition is formed by a licorice extract, a honeysuckle extract and a magnolia bark extract through compound combination. The cosmetic provided by the invention has good bacteriostatic action, particularly having synergistic effect on a side of inhibiting escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger so as to reduce additive amount of former chemical preservatives, and enable products to have antibacterial and anti-inflammatory effects at the same time.

The invention relates to the biological treatment for oil extraction wastewater, which in detail relates to a bacterial agent for treating thick oil wastewater and the method for preparing the same. The comprised components and their proportion by weight are as follows: pseudomonas aeruginosa 5-10%, bacillus subtilis 10-15%, lichen bacilli 5- 15%, Citrobacter propionate tumefaciens 10- 15%, liquid gold tumefaciens 10- 20%, annular gemma bacillus 5- 10%, wilting Bacillus pumilus 5- 10%, spherical arthrobacter 5- 10%, crabstick tumefaciens 5- 10%, and hot ground anaerobic rod baceria 10- 15%. It is prepared by activating bacteria, shake-flask culturing, expanding propagating and mixing. The cooperation action is strong, the biological surface activating agent generating- bacteria can enlarge the dissolution of hydrocarbon petroleum in water and make it is easier for hydrocarbon petroleum to contact with bacteria; the anaerobic bacteria can improve the biodegradation of thick oil wastewater; the degradation perfomace of aerobic bacteria is good and suitable for biological treatment for thick oil wastewater.

The invention relates to a microorganism, of the order of lactic acid bacteria or analog, fragment, derivative, mutant or combination thereof. Said microorganism, or analog, fragment, derivative, mutants or combination thereof can be co-aggregated with at least Staphylococcus aureus or Pseudomonas aeruginosa.

The invention relates to a preparation method of rhamnolipid biological fermentation liquid. The method comprises the following steps: extracting and purifying Pseudomonas aeruginosa obtained from petroleum-contaminated soil, and obtaining Pseudomonas aeruginosa VTS-1 (Pseudomonas sp. -1) The preservation number is: CGMCC2200, and the specific fermentation and cultivation steps are as follows: A. Strain VTS-1 is cultured in shake flasks; B. Primary fermentation cultivation; C. Secondary fermentation cultivation; D. Fermentation cultivation. The process of the method has the advantages of high yield, high efficiency, high loading coefficient, short fermentation cycle, simple process, reduced overall product cost, and is completely suitable for industrial production, and has played a role in the promotion and large-scale application of rhamnolipid biosurfactants. important role.

The invention discloses a compound microorganism preparation. The compound microorganism preparation comprises thermophilic fat bacillus, pseudomonas aeruginosa, streptococcus thermophilus, bacillus megatherium and bacillus flexus. The invention further discloses a preparing method of the compound microorganism preparation and application of the compound microorganism preparation to processing animal carcasses. Compared with the prior art, the compound microorganism preparation has the beneficial effects that macromolecular compounds, such as fat and celluloses in the animal carcasses and straw auxiliary materials, which are difficult to decompose can be efficiently and rapidly decomposed through the compound microorganism preparation, and the animal carcasses are converted into organic fertilizers with the low fat content; and the compound microorganism preparation further has the efficient deodorization function, the release amount of NH3 and H2S in the processing procedure can be effectively reduced, the stink concentration is reduced, and odorless processing is achieved. Finally, the animal carcasses are efficiently and safely processed into the organic fertilizers in an environmental protection manner, and resource utilization of the animal carcasses is achieved.

The invention discloses a solid microbial inoculum that is used for decomposing petroleum pollutants and petroleum products, and a preparation method and applications of the solid microbial inoculum; and the invention belongs to the technical field of biological reparation in the emergency processing of accidents including petroleum pollution of soils and petroleum product leakage. The solid microbial inoculum is prepared by mixing a pseudomonas aeruginosa bacteria fermented fluid, turfy soil and bran with proportion by weight: 0.1-1:1:0.2-0.5, and the effective living bacteria number of pseudomonas aeruginosa bacteria inside the pseudomonas aeruginosa bacteria fermented fluid is 10 to 10 in each milliliter. The solid microbial inoculum has the advantages of high liveness in soil and quick growth, does not cause secondary environmental pollution, and can be used in the biological reparation on the spot of accidents including petroleum pollution of soils and petroleum product leakage.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The present invention provides pharmaceutical compositions in the form of relatively high molecular weight biocompatible polymers such as polyethylene glycol, optionally supplemented with a protective polymer such ad dextran and / or essential pathogen nutrients such as L-glutantine. Also provided are methods for preventing or treating gut-derived sepsis attributable to intestinal pathogens such as Pseudomonas aeruginosa by administering high molecular weight polyethylene glycol as well as methods for monitoring the administration of high molecular weight polyethylene glycol, such as in methods of preventing, ameliorating or treating microbe-induced epithelial disorders, as exemplified by gut-derived sepsis. Frequently, gut-derived sepsis arises as a complication in mammals recovering from surgical intervention or suffering from a disease or disorder, providing indications of suitable animals to receive preventative treatment. Finally, the invention provides a composition comprising infant formula and polyethylene glycol and methods for using that composition.

The invention discloses electric-heating liquid mosquito-repellent incense with natural plant fragrance of agilawood and a preparation method of the electric-heating liquid mosquito-repellent incense. The preparation method comprises the following steps: after uniformly stirring and dispersing 40-60 parts of ethanol, 10-30 parts of butyric acid butylester, 10-15 parts of a traditional Chinese medicine mosquito-repellent purified liquid, 18-19 parts of eucalyptus oil, 1-2 parts of isopropyl myristate, 10-15 parts of fatty alcohol polyoxyethylene ether sodium sulfate and 0.1-0.3 part of sodium benzoate in parts by weight, feeding the raw materials into a bottle, inserting a volatilizing bar made from fiber into the bottle, and heating, so as to volatilize the electric-heating liquid mosquito-repellent incense to repel mosquitoes. The electric-heating liquid mosquito-repellent incense has high safety performance, can be used for effectively repelling the mosquitoes and further has certain inhibiting and killing effects on multiple germs and fungi such as staphylococcus, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria and gram-positive bacteria; meanwhile, the electric-heating liquid mosquito-repellent incense has fragrance and mild properties, can refresh mind, has the effects of relieving pressure and effectively improving sleep quality and is suitable for crowds of all ages.

The invention belongs to the treatment field of heavy metal polluted wastewater and particularly discloses immobilization pseudomonas aeruginosa. The immobilization pseudomonas aeruginosa comprises pseudomonas aeruginosa live bacteria and a mixing carrier used for fixing the bacteria, wherein the mixing carrier is a spherical object embedded with the live bacteria and mainly comprises the following compositions of polyvinyl alcohol, calcium alginate and multi-wall carbon nano-tube in the mass ratio of 50-60:30-40:1.6-6. The preparation method of the immobilization pseudomonas aeruginosa comprises the following steps of: firstly, preparing a mixed solution by mixing the polyvinyl alcohol, the calcium alginate, the multi-wall carbon nano-tube and water, uniformly heating and stirring, and obtaining an immobilization reagent by standing; secondly, adding a pseudomonas aeruginosa into an immobilization reagent to obtain a bacteria solution, and dripping the bacteria solution into a calcium nitration solution to obtain an immobilization pseudomonas aeruginosa microsphere; and finally, obtaining the immobilization pseudomonas aeruginosa through frozen-thawed treatment. The immobilization pseudomonas aeruginosa can reduce hexavalent chromium to trivalent chromium, has the advantages of high efficiency, favorable effect, also simple operation and no secondary pollution.