Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same

a technology of nanosilver and antibacterial granules, which is applied in the directions of plant/algae/fungi/lichens ingredients, biocide, unknown materials, etc., can solve the problems of limited use of silver ions in solution, inability to ensure contact between microbial cells and silver ions, and inability to acquire resistance to metals

Inactive Publication Date: 2002-05-02
LEGEND WIN FINANCE
View PDF0 Cites 74 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The NAGs demonstrate high antibacterial and antifungal activity against a wide range of pathogens with prolonged effectiveness, are non-toxic and non-allergic, and can be safely used in various healthcare and industrial products, including ointments, disinfectants, and food preservatives, while simplifying the production process.

Problems solved by technology

Bacteria treated by these metals do not acquire resistance to the metals.
However, the contact between microbial cells and silver ions is not ensured as the silver ions quickly become eluted in the solution.
Silver ions in solution are difficult to handle and therefore of limited use.
Such solid supports using synthetic polymer materials have been not widely adapted for medical and healthcare purposes.
These materials also do not have sufficiently high antibacterial activity due to the lack of sufficient surface contact with the bacteria.
Additionally, the bactericidal activity of these materials rapidly diminishes as the silver ions become separated from the solid supports, thus, these materials do not show bactericidal activity over a prolonged period of time.
Lastly, the processes for making these materials are complicated and time-consuming.
However, the nitrates irritate the skin.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same

Examples

Experimental program
Comparison scheme
Effect test

example 2

Antibacterial Effect of the Solution Containing the NAGs

[0064] The antibacterial effect of the NAGs in the solution was tested as follows:

[0065] A. Preparation of the NAGs solution

[0066] Five (5) g of the NAGs were suspended in 500 ml of water and stayed in the solution for 24 hrs to obtain an NAGs-containing solution.

[0067] B. Preparation of bacteria

[0068] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120, Escherichia coli stock E109, and Pseudomonas aeruginosa stock PS208 were used for the testing. These stocks were provided by the Microbiology Teaching and Research Section at Henan Medical University.

[0069] The frozen stocks of bacteria were seeded onto solid culture media. Single colonies were picked and inoculated respectively in 2 ml liquid culture medium and cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in refrigerator (around 0.degree. C.)...

example 3

Time Course of Antibacterial Effects of the NAGs

[0074] A time course study of the antibacterial effects of the NAGs in a solution was conducted as follows:

[0075] A. Preparation of bacterial stocks

[0076] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120, Escherichia coli stock E109, and Pseudomonas aeruginosa stock PS208 were provided by the Microbiology Teaching and Research Section at Henan Medical University. The stocks were cultured in an incubator at 37.degree. C. for 24 hours.

[0077] The frozen stocks of bacteria were seeded to a culture plate. Single colonies were chosen and inoculated in 2 ml liquid culture medium, respectively, where they were cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in the refrigerator (around 0.degree. C.) until ready for use. The solution contained about 10.sup.5 CFU / ml bacterial stock.

[0078] B. The effect of the NA...

example 4

Antibacterial Effect of the NAGs on Toothbrush

[0085] The antibacterial effect of the NAGs was tested on toothbrush as follows:

[0086] A. Preparation of bacteria

[0087] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120 was provided by the Microbiology Teaching and Research Section at Henan Medical University. The stock was cultured in an incubator at 37.degree. C. for 24 hours.

[0088] The frozen stock of bacteria was seeded to a culture plate. A single colony was chosen and inoculated in 2 ml liquid culture medium, respectively, where the bacteria were cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in the refrigerator (around 0.degree. C.) until ready for use. The solution contained about 10.sup.5 CFU / ml bacterial stock.

[0089] B. Treatment of toothbrush

[0090] The bactericidal activity of the NAGs on toothbrush was tested by two (2) methods:

[0091] (1). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effuses L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 mn in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

Description

[0001] This application claims the priority of U.S. Provisional Application No. 60 / 230,925, filed on Sep. 13, 2000, which is herein incorporated by reference.[0002] The present invention relates to nanosilver particles-containing antibacterial and antifungal granules (NAGs). The nanosilver particles are attached to the surfaces and pores of stalk marrow of Juncus effuses L, which acts as an inert carrier for nanosilver. Each of the nanosilver particles contains a metallic silver core which is surrounded by silver oxide. The size of the nanosilver particle is between 1-100 nm in diameter. The present invention also relates to methods for preparing the NAGs and for using the NAGs. The NAGs can be used in a variety of healthcare, medicinal and industrial products.DESCRIPTION OF THE RELATED ART[0003] Metals including silver, copper, mercury, and zinc are known for anti-bacterial properties. Bacteria treated by these metals do not acquire resistance to the metals. Therefore, the bacteric...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K33/38
CPCA61K33/38Y02A50/30
Inventor YAN, JIXIONGCHENG, JIACHONG
Owner LEGEND WIN FINANCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com