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A Strain of Bacillus and Its Application

A technology of bacteria strain and utilization rate, applied in the direction of application, bacteria, soil conditioning materials, etc.

Active Publication Date: 2020-02-11
青岛秾贝尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

while the strain Bacillus horneckiae Belongs to the genus Bacillus, rare related reports in China

Method used

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  • A Strain of Bacillus and Its Application
  • A Strain of Bacillus and Its Application
  • A Strain of Bacillus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Strains Bacillus horneckiae Isolation and Identification of DSM23495

[0021] (1) Strain isolation

[0022] Strains of the invention Bacillus horneckiae DSM23495 is obtained from the soil by means of plate dilution coating and streaking. Separation method: (1) Medium: Mengjina inorganic phosphorus solid medium; (2) Soil sample collection: The collection location is Jiaozhou Bay Base, Qingdao Peng wetland, using 5-point sampling method; (3) Separation step: Weigh 1 g soil sample in 100 mL sterile water, place in a shaker at 30°C, 150 r / min for 10 minutes, and dilute to 10 -2 、10 -3 、10 -4 concentration. Pipette 100 μL of the above diluted solution, and evenly spread it on the surface of Montina inorganic phosphorus solid medium plate, apply three parallels for each gradient, and incubate at 30°C for 7 days, observe whether there is a phosphorus-dissolving circle, according to the diameter of the phosphorus-dissolving circle ( D) The ratio of the colo...

Embodiment 2

[0029] Embodiment 2: Determination of phosphorus dissolving ability

[0030] (1) The phosphate solubilizing ability of phosphate solubilizing bacteria was determined by Montina inorganic phosphorus liquid medium.

[0031] Mengkina inorganic phosphorus liquid medium formula: glucose 10g, (NH 4 ) 2 SO 4 0.5 g, NaCl 0.3 g, KCl 0.3 g, FeSO 4 ·7H 2 O 0.03 g, MgSO 4 ·7H 2 O 0.3 g, MnSO 4 4H 2 O 0.03 g, Ca 3 (PO 3 ) 2 10 g, 1000 mL of distilled water, adjusted to pH 7.0, can be used to quantitatively determine the phosphorus-solubilizing ability of phosphorus-solubilizing microorganisms.

[0032] Strains preserved on the slant Bacillus horneckiae DSM23495 was inoculated into NA liquid medium, and shaken at 30°C and 180 r / min for 12 h to obtain a seed solution; the above seed solution was inoculated into 100 mL of Mongolian gold at a 1% inoculum volume (v / v). Na inorganic phosphorus liquid medium in the Erlenmeyer flask; and according to the same inoculum amount, inocu...

Embodiment 3

[0036] Example 3: Strains Bacillus horneckiae Liquid scale fermentation of DSM23495

[0037] (1) Strain activation: strain Bacillus horneckiae DSM23495 was stored on the slant of NA medium at -20°C. Before use, the strain was inoculated on the surface of NA medium by streaking, and activated twice for later use.

[0038] (2) Seed shake flask: the activated strain Bacillus horneckiae DSM23495 was inoculated into a Erlenmeyer flask containing NA liquid medium, and cultured with shaking at 30°C and 200 r / min for 12 h;

[0039] (3) Seed tank fermentation: Inoculate the shake flask seed solution prepared in step (1) into the seed tank, and ferment until the bacteria enter the logarithmic growth phase;

[0040] Seed tank liquid fermentation medium, formula: 15 g corn flour, 4 g glucose, 21 g bean cake powder, 4 g fish meal, CaCO 3 9 g, (NH 4 ) 2 SO 4 1.5 g, K 2 HPO 4 0.5 g, MgSO 4 ·7H 2 O 0.1 g, NaCl 0.1 g, water 1000 mL, pH adjusted to 7.5.

[0041] Fermentation...

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Abstract

The present invention discloses a strain Bacillus horneckiae The invention and its application belong to the technical field of phosphorus-dissolving microbial fertilizers. The strain has been preserved in the China General Microorganism Culture Collection Management Center. The preservation date is January 9, 2017, and the preservation number is CGMCC No.13551. The invention also discloses a microbial agent containing the bacterial strain and a preparation method thereof. Experiments have shown that the strain has the characteristics of fast growth, strong phosphorus-dissolving ability, and rapid colonization in the rhizosphere of plants; the microbial agent prepared by the strain has phosphorus-dissolving function, can improve soil structure, and is beneficial to increase the yield of phosphorus fertilizer. The utilization rate is of great significance to improve the yield and quality of crops.

Description

technical field [0001] The invention belongs to the field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a phosphorus-dissolving Bacillus horneckiae and its use, and the adoption of the Bacillus horneckiae The prepared phosphate-solubilizing microbial bacterial agent. Background technique [0002] As one of the three major nutritional elements necessary for plant growth and development, phosphorus is the basic element of key components such as DNA and ATP in plant cells, and participates in photosynthesis and biochemical metabolism of plants at the same time. Therefore, phosphorus is one of the most important nutrient elements in crop planting and production, but at the same time phosphorus is also a resource that is difficult to regenerate in the soil. According to statistics, about 2 / 3 of the cultivated land in my country lacks phosphorus resources, and more than 95% of phosphorus and Ca 2+ , Fe 2+ , Fe 3+ with A1 3...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/20C12N1/205C12R2001/07
Inventor 杜丰玉牛赡光肖琳周远明王学军
Owner 青岛秾贝尔生物工程有限公司
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