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Phosphate solubilizing bacteria PC05 and isolating and screening method thereof

A technology for separating, screening, and phosphate-solubilizing bacteria, applied in the field of agricultural microorganisms, can solve problems such as the inability of plants to directly absorb and utilize phosphate, uneven phosphate-solubilizing ability, and imbalance of nutrient elements, so as to reduce soil erosion, improve phosphate fertilizer utilization efficiency, and improve effective The effect of phosphorus content

Inactive Publication Date: 2017-05-31
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus-deficient soil generally refers to the soil with soil available phosphorus (P) less than 10mg / kg. The area of ​​phosphorus-deficient soil in my country exceeds 74% of the total cultivated land area. Most of the phosphorus in the soil exists in the form of insoluble phosphate, and plants cannot directly absorption
In order to meet the needs of production, most of the cultivated fields in my country need to supplement a large amount of phosphorus chemical fertilizers. Due to long-term accumulation and enrichment, a series of production and ecological problems such as soil compaction and nutrient element imbalance have been caused. Therefore, how to improve and improve my country's cultivation The utilization rate of phosphorus in field soil is still a strategic issue
Judging from the existing literature reports, there are many and complex types of phosphate-solubilizing bacteria, and their phosphorus-solubilizing ability is also uneven, and the strains used in actual production are few and lack innovation. From this perspective, screening has a strong phosphorus-solubilizing ability Work on wild strains remains significant

Method used

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  • Phosphate solubilizing bacteria PC05 and isolating and screening method thereof
  • Phosphate solubilizing bacteria PC05 and isolating and screening method thereof
  • Phosphate solubilizing bacteria PC05 and isolating and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of inorganic phosphorus solid medium: by weight, the medium is composed of the following components: 10 g of glucose, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·H 2 O0.03g, Ca 3 (PO 4 ) 2 5g, agar 20g, distilled water 1000g; Ca 3 (PO 4 ) 2 After being sterilized separately from the rest of the ingredients, mixed, and adjusted to pH 7.0 to 7.2.

[0017] Take 10 mL of pig fecal liquid sample, put it into a 250 mL conical flask with glass beads, add 90 mL of sterile water, and shake at a constant temperature of 28 to 30 ° C for 30 to 45 minutes, so that the sample is evenly dispersed in the sterile water, and the dilution ratio is 10 -1 After the bacterial suspension was allowed to stand, the supernatant was taken and diluted in turn to obtain 10 -3 , 10 -5 , 10 -7 10 times the concentration gradient of the sample bacterial suspension, 10 -3 , 10 -5 , 10 -7 Take 0.1 mL of each bacterial susp...

Embodiment 2

[0022] Morphological observation and molecular identification of Phosphorus solubilizing bacteria PC05 isolated and screened in Example 1 were carried out. The growth physiology of Phosphorus solubilizing bacteria PC05 is that the colony surface is smooth, translucent, with neat edges, round and convex, and the Phosphorus solubilization circle is not obvious in the early stage of culture, but after 3 to 4 days, the Phosphorus solubilization circle is more obvious; the use of primers 27F and 1492R to strain PC05 Amplification and forward sequencing were performed to obtain a sequence length of 1445bp. By BLAST (Basic Search Tool for Local Sequence Alignment), the sequence was compared with the sequence in the GenBank database. Its similarity with NR_112560.1 exceeded 99%, and it was identified as Inquilinus ginsengisoli.

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Abstract

The invention discloses phosphate solubilizing bacteria PC05 and an isolating and screening method thereof. The strain name of the phosphate solubilizing bacteria PC05 is Inquilinus ginsengisoli PC05. The phosphate solubilizing bacteria PC05 are preserved in China Center for Type Culture Collection on June 24, 2016 in Wuhan University which is in Wuhan, China, with the preservation number of CCTCC NO:M2016347. The cultivated phosphate solubilizing bacteria PC05 can enrich wild phosphate solubilizing bacteria genetic resources, have high phosphate solubilizing capacity, and can increase the content of available phosphorus in soil, increase the utilization efficiency of insoluble phosphorus in soil, and reduce the application amount of phosphatic fertilizer.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a phosphate solubilizing bacteria PC05 and a method for separating and screening the same. Background technique [0002] The content of phosphorus in plants is second only to nitrogen and potassium, and it is one of the key nutrients for plant growth and development. Phosphorus-deficient soil generally refers to soil with soil available phosphorus (P) less than 10 mg / kg. The area of ​​phosphorus-deficient soil in my country exceeds 74% of the total cultivated land area. Most of the phosphorus in soil exists in the form of insoluble phosphate, and plants cannot directly Absorb and utilize. In order to meet the needs of production, most of the cultivated fields in our country must be supplemented with a large amount of phosphorus fertilizers. Due to long-term accumulation and enrichment, a series of production and ecological problems such as soil compaction and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/68C12Q1/04C12R1/01
CPCC12N1/02C12N1/20C12Q1/6869C12Q2531/113
Inventor 肖乃东李震宇朱端卫周洋周文兵蔡建波肖雪杨特武
Owner HUAZHONG AGRI UNIV
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