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A kind of phosphate solubilizing bacteria pc05 and its isolation and screening method

A phosphate-solubilizing bacteria and a typical technology, applied in the field of phosphate-solubilizing bacteria PC05 and its separation and screening, can solve the problems such as the inability of plants to directly absorb and utilize, uneven phosphorus-solubilizing ability, and imbalance of nutrient elements, so as to reduce soil erosion and improve phosphorus fertilizer. Efficiency of utilization and effect of increasing the content of available phosphorus

Inactive Publication Date: 2020-07-31
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus-deficient soil generally refers to the soil with soil available phosphorus (P) less than 10mg / kg. The area of ​​phosphorus-deficient soil in my country exceeds 74% of the total cultivated land area. Most of the phosphorus in the soil exists in the form of insoluble phosphate, and plants cannot directly absorption
In order to meet the needs of production, most of the cultivated fields in my country need to supplement a large amount of phosphorus chemical fertilizers. Due to long-term accumulation and enrichment, a series of production and ecological problems such as soil compaction and nutrient element imbalance have been caused. Therefore, how to improve and improve my country's cultivation The utilization rate of phosphorus in field soil is still a strategic issue
Judging from the existing literature reports, there are many and complex types of phosphate-solubilizing bacteria, and their phosphorus-solubilizing ability is also uneven, and the strains used in actual production are few and lack innovation. From this perspective, screening has a strong phosphorus-solubilizing ability Work on wild strains remains significant

Method used

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  • A kind of phosphate solubilizing bacteria pc05 and its isolation and screening method
  • A kind of phosphate solubilizing bacteria pc05 and its isolation and screening method
  • A kind of phosphate solubilizing bacteria pc05 and its isolation and screening method

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Experimental program
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Effect test

Embodiment 1

[0016] The preparation of inorganic phosphorus solid medium: by weight, medium is made up of following composition: glucose 10g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·H 2 O0.03g, Ca 3 (PO 4 ) 2 5g, agar 20g, distilled water 1000g; 3 (PO 4 ) 2 Separately sterilize and mix with the rest of the ingredients, and adjust the pH to 7.0-7.2.

[0017] Take 10mL of pig manure liquid sample, put it into a 250mL Erlenmeyer flask with glass beads, add 90mL of sterile water, and shake at a constant temperature of 28-30°C for 30-45min, so that the sample is evenly dispersed in sterile water, and the dilution factor is 10 -1 The bacterial suspension, the supernatant was taken after the bacterial suspension was allowed to stand, and diluted successively to obtain 10 -3 、10 -5 、10 -7 Bacteria suspension sample with double concentration gradient, 10 -3 、10 -5 、10 -7 Take 0.1mL of the bacterial suspension with a double co...

Embodiment 2

[0022] Morphological observation and molecular identification were carried out on the phosphosolubilizer PC05 isolated and screened in Example 1. Phosphorus solubilising bacteria PC05 has a growth physiological form with smooth colony surface, translucent, neat edges, and round convex shape. The phosphorus-dissolving circle is not obvious at the initial stage of culture, but the phosphorus-dissolving circle is more obvious after 3-4 days; the use of primers 27F and 1492R on strain PC05 Amplification and forward sequencing were carried out to obtain a sequence length of 1445bp. The sequence was compared with the sequence in the GenBank database through BLAST (local sequence alignment basic search tool), and its similarity with NR_112560.1 exceeded 99%, and it was identified as Inquilinus ginsengisoli.

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Abstract

The invention discloses phosphate solubilizing bacteria PC05 and an isolating and screening method thereof. The strain name of the phosphate solubilizing bacteria PC05 is Inquilinus ginsengisoli PC05. The phosphate solubilizing bacteria PC05 are preserved in China Center for Type Culture Collection on June 24, 2016 in Wuhan University which is in Wuhan, China, with the preservation number of CCTCC NO:M2016347. The cultivated phosphate solubilizing bacteria PC05 can enrich wild phosphate solubilizing bacteria genetic resources, have high phosphate solubilizing capacity, and can increase the content of available phosphorus in soil, increase the utilization efficiency of insoluble phosphorus in soil, and reduce the application amount of phosphatic fertilizer.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a phosphate-solubilizing bacterium PC05 and a separation and screening method thereof. Background technique [0002] The content of phosphorus in plants is second only to nitrogen and potassium, and it is one of the key nutrients for plant growth and development. Phosphorus-deficient soil generally refers to the soil with soil available phosphorus (P) less than 10mg / kg. The area of ​​phosphorus-deficient soil in my country exceeds 74% of the total cultivated land area. Most of the phosphorus in the soil exists in the form of insoluble phosphate, and plants cannot directly Absorb and utilize. In order to meet the needs of production, most of the cultivated fields in my country need to supplement a large amount of phosphorus chemical fertilizers. Due to long-term accumulation and enrichment, a series of production and ecological problems such as soil compaction ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/6869C12Q1/04C12R1/01
CPCC12N1/02C12N1/20C12Q1/6869C12Q2531/113
Inventor 肖乃东李震宇朱端卫周洋周文兵蔡建波肖雪杨特武
Owner HUAZHONG AGRI UNIV
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