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Pantoea dispersa and application thereof in preparation of microbial fertilizer

A technique for dispersing Pantoea and microorganisms, applied in the field of microorganisms, can solve problems affecting crop yield, water eutrophication, soil salinization, etc., and achieve the effects of avoiding soil structure problems, fast growth, and promoting plant growth

Active Publication Date: 2018-03-27
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The long-term retention of chemical fertilizers in the soil will not only seriously affect the yield of crops, but also cause soil structure problems such as soil salinization, acidification, and compaction. environmental pollution

Method used

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  • Pantoea dispersa and application thereof in preparation of microbial fertilizer
  • Pantoea dispersa and application thereof in preparation of microbial fertilizer
  • Pantoea dispersa and application thereof in preparation of microbial fertilizer

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Embodiment Construction

[0028] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0029] 1. Isolation and screening of strains

[0030] On July 4, 2017, we collected some peanut leaves from a peanut field in Zhongmu County, Zhengzhou, Henan (34°44′48.22″N, 114°2′17.22″E).

[0031] Weigh 1g of peanut leaves, wash them twice with sterile water, then use sterile filter paper to absorb excess water on the leaf surface, then cut the leaves into 1cm-2cm pieces with a sterile knife, and put the cut leaves in the Add 9mL of sterile water to the grinder to start grinding. After grinding fully, let it stand for 30 minutes, mix the ground mixture thoroughly, and then gradiently dilute it for 10 -1 ~10 -5 , take 10 -3 、10 -4 、10 -5 Plate coating with three gradients, respectively coating on organic phosphorus solid plate and inorganic phosphorus solid plate, each coating in three parallels, and then cultured upside down in a ...

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Abstract

The invention discloses pantoea dispersa and an application thereof in preparation of a microbial fertilizer, wherein the pantoea dispersa is classified and named as pantoea dispersa, is preserved inChina General Microbiological Culture Collection Center, has the preservation number of CGMCC No.14876, and is preserved on November 14th, 2017; and the pantoea dispersa is specifically applied for seed blending in preparation of the microbial fertilizer. The pantoea dispersa has the beneficial effects: (1) the pantoea dispersa has fast growth rate and strong phosphorus removal ability, and not only can dissolve insoluble organophosphorus but also can dissolve insoluble inorganic phosphorus; (2) the pantoea dispersa has strong stress resistance and can grow in an LB culture medium with the salinity of 6%; (3) the pantoea dispersa can promote development of plant root systems and promote growth of plants after being mixed with corn seeds; and (4) the pantoea dispersa used in cultivated landcan improve the utilization rate of soil phosphorus elements, reduces the use of chemical fertilizers, and avoids the problem of soil structure.

Description

technical field [0001] The invention relates to a strain of bacteria and its application, in particular to a strain of Pantoea dispersa and its application in the preparation of microbial fertilizers, belonging to the technical field of microorganisms. Background technique [0002] Phosphorus is an element in great demand during plant growth. It can not only promote plant root development and plant growth, but also enhance crop resistance to cold, drought, disease, and lodging. [0003] At present, more than 70% of the cultivated land in our country is deficient in phosphorus. Although nitrogen, phosphorus, potassium and other chemical fertilizers are used on these cultivated lands, most phosphorus elements are difficult to form due to the combination of most phosphorus elements with iron ions, aluminum ions, and calcium ions in the soil. Only 10-20% of soluble phosphate is utilized, and the utilization rate is very low. [0004] Since the use of chemical phosphate fertiliz...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/205C12R2001/01
Inventor 邹秋霞蒋先芝王科晶金漫
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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