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Root nodule endophyte S43 with phosphate solubilizing function and application of root nodule endophyte S43

An endophyte and phosphorus solution technology, applied in the field of agricultural microorganisms, can solve the problems of few and few and lack of research on plant endophytes

Pending Publication Date: 2022-05-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN112322535A discloses a kind of Pseudomonas phosphatilytica and its application, and isolates a new bacterial strain capable of efficiently dissolving insoluble calcium phosphate: Pseudomonas phosphatilytica (Pseudomonas phosphatilytica) PD171; Most of the phosphate-solubilizing bacteria are isolated from the soil, and the research on plant endophytes is relatively lacking, and the strains of plant endophytic phosphate-solubilizing bacteria obtained by screening are even less; Resource bank, the study of new plant endophytes is of great significance for the application of plant uptake and utilization and improvement of soil fertility

Method used

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  • Root nodule endophyte S43 with phosphate solubilizing function and application of root nodule endophyte S43
  • Root nodule endophyte S43 with phosphate solubilizing function and application of root nodule endophyte S43
  • Root nodule endophyte S43 with phosphate solubilizing function and application of root nodule endophyte S43

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Experimental program
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Effect test

Embodiment 1

[0038] The isolation of embodiment 1 bacterial strain

[0039] (1) Sample collection

[0040] The samples came from Nahui Village (Lat.24.514341N, Lon.104.590378E) in Xingyi City, Guizhou Province.

[0041] (2) Surface disinfection of root nodule samples

[0042] Collect the fresh root nodules of the green leafy locust in a clean triangular flask, soak in sterile water for 5 minutes and rinse 3 to 5 times, add 75% alcohol to soak for 3 minutes and shake slightly, rinse with sterile water for 3 to 5 times, then add 3.5% The surface was disinfected with NaClO for 8 minutes, and finally rinsed with sterile water for 5 to 8 times.

[0043] (3) Isolation and culture of endophytic bacteria in rhizobia

[0044] Place the sterilized nodule samples in a 5ml centrifuge tube, crush the nodules with a sterilized glass rod, and squeeze to obtain nodule juice. Under sterile conditions, the root nodule juice after detoxification treatment was diluted to obtain 10 -3 、10 -4 and 10 -5 S...

Embodiment 2

[0047] The identification of embodiment 2 bacterial strains

[0048] (1) Colony characteristics of the strain

[0049] The strain S43 isolated above was identified to be rod-shaped, with a colony length of 1 μm-2.5 μm and a width of about 0.5 μm. It is a regular circle on the YMA plate, the surface is wet and sticky, shiny, and the whole is white.

[0050] (2) Biochemical indicators of bacterial strains

[0051] Physiological and biochemical index determination of the bacterial strain shows that the bacterial strain S43 is negative for Gram staining, and the results are as follows: figure 2 Shown; strain S43 is positive for citrate, negative for starch hydrolysis, positive for hydrogen secretion, positive for gelatin, negative for 3-ketolactose, the results are as follows image 3 shown.

[0052] (3) Identification of strains

[0053] Extract the genomic DNA of bacterial strain S43 isolated in Example 1, and use the extracted DNA as a template to perform 16SrDNA PCR ampl...

Embodiment 3

[0060] Example 3 Phosphate-dissolving and hydrogen-releasing performance identification of bacterial strain S43

[0061] (1) Identification of Phosphorus Solubilizing Ability of Strain S43

[0062] In Example 1, the inorganic phosphorus medium was used for preliminary screening, and the obtained bacterial strain S43 with obvious phosphorus-dissolving circle was further measured, and the content of soluble phosphorus in the medium was determined by the molybdenum-antimony anti-colorimetric method to determine its phosphorus-dissolving ability. .

[0063] Inoculate the strain S43 into a Erlenmeyer flask containing 50ml of inorganic phosphorus liquid medium, adjust the initial pH to 7.0, place it in a shaker at 28°C and 180rpm for cultivation, and measure the culture at 6h, 12h, 24h and 48h respectively. The soluble phosphorus content in the base was measured every 48 hours thereafter. Determination result takes cultivation time as abscissa, and soluble phosphorus content is or...

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Abstract

The invention provides a root nodule endophyte S43 with a phosphate solubilizing function and application of the root nodule endophyte S43. A root nodule endophyte pseudomonas koreensis S43 strain is obtained through separation, the strain is preserved in Guangdong Microbial Culture Collection Center on December 8, 2021, and the preservation number is GDMCC No: 62100. Researches show that the strain has strong phosphate solubilizing ability, the content of soluble phosphorus in supernate of the strain after fermentation reaches 400 mu g / ml, and the content of the soluble phosphorus is increased along with the prolonging of fermentation time; in addition, the strain S43 also has hydrogen secreting capacity, can reduce the pH value of a culture medium from 7.0 to 4.13, is beneficial to converting insoluble phosphorus into soluble phosphorus which can be absorbed and utilized by plants, and promotes absorption and utilization of crops. According to the invention, a new degrading bacterium selection is provided for a soil phosphorus-dissolving microbial strain, a strain resource library of phosphorus-dissolving bacteria is expanded, and absorption and utilization of phosphorus by plants and improvement of soil fertility are facilitated.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms. More specifically, it relates to a rhizobium endophyte S43 with the function of dissolving phosphorus and its application. Background technique [0002] Phosphorus is one of the macronutrient elements required for plant growth and development, and its content in plants is second only to nitrogen and potassium. In the process of planting in agricultural production, because phosphorus is easy to form insoluble phosphate with iron, aluminum, calcium and other elements in the soil, it is difficult for plants to directly use and absorb it. Phosphorus-deficient plants are short and stunted or even stagnant. In order to meet the production requirements, most of the farmland must apply phosphorus fertilizers, and the utilization rate of phosphorus fertilizers is low, which in turn aggravates the consumption of phosphorus resources, and the long-term accumulation of phosphorus leads t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C12R1/38
CPCC12N1/20C05F11/08C05G3/00
Inventor 陆星赵谦田江梁翠月
Owner SOUTH CHINA AGRI UNIV
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