41 results about "Replantation" patented technology

The invention relates to an apparatus for film peeling and replantation. According to the invention, the apparatus for film peeling and replantation comprises a fixing plate (102) for fixing a sheet component where a film is attached, a peeling roller (104) which is arranged above the fixing plate (102) and to which one side of the film is attached, a peeling roller moving unit(106) which moves the peeling roller (104) where one side of the film is attached so as to peel the film off the sheet component, an adhesive roller (108) which attaches a film separated from the peeling roller and retains the other side attaching to the sheet component, and an adhesive roller conveying unit(112) which moves the adhesive roller (108) to attach the peeled film to the sheet component again.

Methods and apparatus for preserving detached tissues, especially digits and limbs, which are detached as a result of traumatic amputation. By using the methods and apparatus, detached tissues can be preserved for greater lengths of time and are ultimately in a better condition for replantation surgery.

The invention provides a Korean pseudomonas and application thereof. The Korean pseudomonas is identified as Korean pseudomonas CJ-361, and the preservation number is CGMCC NO.12122. The Korean pseudomonas CJ-361 is obtained through separation from radix pseudostellariae rhizosphere soil by adopting a pseudomonas selectivity culture medium, an antagonistic experiment is performed on fusarium oxysporum and fusarium moniliforme separated to the radix pseudostellariae root lesion portion through an agar plate opposite culture method, and it is found that the Korean pseudomonas has the strong antagonism on the fusarium oxysporum and the fusarium moniliforme. Meanwhile, the invention provides the optimum culture medium for antagonistic bacterium growth, the optimum formula is 1 / 4LB+1 / 20MS (containing no ferric salt)+0.1 wt% brown sugar, and the fermented liquor has the obvious antagonistic effect. The antagonistic strain and the microbial agent thereof can effectively prevent and treat radix pseudostellariae root rot diseases, and the environment-friendly, ecological and safe biological prevention and control potential strain is provided for overcoming or relieving replantation diseases of radix pseudostellariae.

The invention relates to a method for constructing tissue engineered cartilage by using a PCL kernel-containing material. The method comprises the steps of a, making a PLC kernel; b, making a PLC kernel-containing PGA / PLA compound ear bracket; c, making a PGA / PLA compound ear bracket; d, culturing cartilage cells; e, constructing cartilage auricle bracket; and f, nude mouse subcutaneous replantation of in-vitro constructed auricle: respectively carrying out nude mouse subcutaneous implantation on the cartilage cells and an ear bracket compound after culturing in vitro for a week. According to the method, the tissue engineered cartilage can be constructed by using the PLC kernel-containing material, so that enough strength is provided to resist the tension caused by the subcutaneous implantation and then the original complicated and precise shape is maintained.

This invention discloses a method for obtaining turnip mosaic antiviral preserved szechuan pickle by means of Hc-Pro gene inversion, the method takes twice subcultured cotyledon inducted wound-healing tissue as inversion receptor, and co-culture the tissue with meloidogynosis peasant bacillus culture liquid with Hc-Pro gene, then continuously sieve the inversed wound-healing tissue on antibiotics added medium, last do differentiation and replantation. Compared with current technics, the beneficial effects of this invention are:Hc-Pro gene inversion is process on cell, and this can improve inversion efficiency and inheritance stability of the gene transferred plant, and can obtain Hc-Pro gene taking plant instead of chimera gene transferred plant.

A disclosed preparation method for self-skull used in replantation technology comprises that the self-skull is obtained for replantation by performing decontamination, pathogen inactivation, deproteinization, degreasing, decellularization, freeze drying, disinfection and the like on self skull taken out after an operation. The preparation method has the advantages that the storage time is long, the appearance of the taken-out self-skull is not changed after the self-skull is processed, no rejection phenomenon exists after implantation, and the like.

The invention discloses an intervertebral fusion fixator, relates to the field of medical instruments, and aims to provide a fusion fixator which is matched with anterior cervical approach vertebral X-shaped excision decompression vertebroplasty and can shorten the bone crawling substitution distance, shorten the fusion time and increase the bone grafting fusion success rate. According to the technical scheme, the intervertebral fusion fixator for X-shaped excision decompression vertebroplasty of the vertebral body comprises a fusion fixing body and screws, the fusion fixing body is provided with three bone grafting cavities, the horizontal section of the first bone grafting cavity is in a fan shape, the outer sides of the two radiuses of the fan shape are the second bone grafting cavity and the third bone grafting cavity respectively, a fixing blocking piece is arranged above or below the arc edge of the fan shape, the fixing blocking piece is provided with a through hole and the first screw, and the first screw is used for being implanted into a replantation bone block; and fixing holes are formed in the arc edge of the first bone grafting cavity, and screws used for implanting residual vertebral bodies on the two sides and adjacent normal vertebral bodies are arranged in the fixing holes. The intervertebral fusion fixator is suitable for the anterior cervical approach vertebral X-shaped excision decompression vertebroplasty.

The invention relates to a transgenosis construct and an application thereof in preparing a space-time adjustable liver damage model and discloses a construct combination used for preparing the space-time adjustable liver damage model. The construct combination comprises a construct expressing space-time adjustable liver damage induction induced gene and a construct expressing Cre recombinant enzyme. The invention also discloses cells containing the construct and a method for preparing the space-time adjustable liver damage model. The obtained animal model can efficiently induce the liver damage at any time, and provides an effective way for the research of hepatic cell transplantation and liver replantation.

The present invention provides a soil remediation organic fertilizer for overcoming a watermelon replantation obstacle, a preparation method thereof and an application thereof. Agricultural straws, a straw-decomposing inoculant, an N fertilizer, a P fertilizer and a K fertilizer are subjected to mixed fermentation to prepare the soil remediation organic fertilizer. The straw-decomposing inoculant is prepared through subjecting four strains to cross antagonism and cultivation. The above four strains are composed of bacillus subtilis, Trichodermareesei, lactobacillus plantarum and Lactobacillus acidophilus. The bacillus subtilis is used for inhibiting and killing harmful bacteria and pathogenic bacteria in the soil. The Trichodermareesei is used for decomposing straw fibers and quickly composting straws. The lactobacillus plantarum is used for inhibiting and killing insects and worm eggs in the soil. The Lactobacillus acidophilus is used for removing the autotoxicity residues of watermelons. The effects of fungicides are realized based on the mutual coordination of the fungicides, agricultural straws, N, P and K fertilizers. Therefore, the accumulation of soil-borne pathogens, the soil nutrient imbalance and the plant autointoxication, which are inducement for replantation and successive cropping obstacles, can be avoided. Meanwhile, soil organic matters are supplemented, and the soil aggregate structure is optimized. Therefore, the soil remediation organic fertilizer has the advantages of water retention, drainage and ventilation, so that the plant root system is developed. Plants are enabled to grow healthily.

The invention provides a skull flap preservation device with an activity maintaining function. The skull flap preservation device with the activity maintaining function comprises a box. One side of the box is open. At least three layers of transverse partition plates are sequentially arranged in a cavity of the box from top to bottom. A plurality of month frames are arranged above each layer of transverse partition plate. At least three layers of pull-type lattice drawers are arranged in each month frame. Carrying sliding strips in contact with the corresponding pull-type lattice drawers are arranged on two opposite inner walls of each month frame. A plurality of vertical partition plates are arranged in each pull-type lattice drawer, and a lattice used for storing a skull flap is formed between every two adjacent vertical partition plates. A month marker is arranged on the front side wall of each month frame. A plurality of layers of markers are arranged on the front side wall of eachlayer of pull-type lattice drawers. By adoption of the skull flap preservation device with the activity maintaining function, the activity of skulls can be effectively maintained, the skulls are protected against contamination from the outside, accurate and rapid positioning is achieved, and errors are avoided; the influence on other skulls when one skull is stored or taken is reduced; and the infection rate after skull replantation is decreased, and the healing rate of patients is increased.

The invention discloses a preparation method of a rapidly decellularized unifoliate liver biological scaffold. An ionic detergent sodium dodecyl sulfonate (SDS) with the problem that thrombus is easy to form after tissue transplantation and the problem of low replantation rate of decellularized tissues in a composite cellularization process is not used; a decellularized left outer-lobe liver biological scaffold is prepared by a weakly alkaline Triton solution perfusion method; due to the addition of NaOH in the Triton solution, the neutral Triton solution is in alkalescence; the cell lysis removal effect is enhanced; the time required for removing cells is shortened; and the optimal concentration and dosage of the NaOH and the Triton solution are adopted, so that the decellularization effect is the best.

Disclosed is a collagen material for promoting the survival of the skin with degloving injuries and avulsion injuries. The collagen material is composed of a collagen sponge and a nutrient solution. Specifically, bovine achilles tendons and pig achilles tendons which serve as raw materials are subjected to enzymatic hydrolysis and purification, collagen is prepared through fine extraction, the collagen sponge is obtained after crosslinking and freeze-drying treatment, and the nutrient solution is obtained. The nutrient solution is added while the collagen sponge is implanted in a wound. The collagen material has the advantages that the collagen material is implanted conveniently and easily applied to the surgical operation, the survival of the skin with the degloving injuries and the avulsion injuries is effectively promoted, the early recovery of the functions of the affected limbs is promoted, the necrosis rate of the skin with the degloving injuries or the avulsion injuries is reduced, the situation that multiple operations are caused by the occurrence of skin necrosis and complications of bone-tendon exposure is avoided, and the surgical complications caused by replantation surgery or flap surgery and the like are reduced to the greatest degree; the collagen material is suitable for hand and foot surgery and treatment of degloving injuries and avulsion injuries of the fourlimbs, and applicable to popularization and application.

The invention discloses a saline-alkali land restoration method by improving soil. The method includes the following steps that 1, flushing is conducted, wherein a trench with the depth of 2.3-2.4 m is dug around to-be-remedied soil, a cement plate is inserted in the trench, a water suction pump is used for pumping water to flush the to-be-remedied soil once every 4-7 days, and flushing is continuously conducted for 7-11 times; 2, the soil is ploughed repeatedly in a turning mode, and a restoration agent is spread, so that the restoration agent is mixed evenly with soil of the saline-alkali land and can enters the bottommost portion of a soil layer and saline-alkali soil in the deep of the ground can be conveniently remedied; 3, at the same time, after crops are harvested, plant ash obtained after incineration returns to the saline-alkali land again, the pH value of the saline-alkali land can be greatly improved, the properties of the land are thus improved, and replantation in the later period is facilitated.

The invention relates to an upper limb abdomen pedicled skin flap fixator, and belongs to medical instruments. The upper limb abdomen pedicled skin flap fixator is used for the treatment of replantation operation of severed limbs, and consists of a base, an arm support plate of which one end is hinged on the base, a bundling belt arranged on the arm support plate and a mechanical lifting mechanism which is connected between the base and the arm support plate, so that the arm support plate is lifted and drops relative to the base. The upper limb abdomen pedicled skin flap fixator has the advantages that an arm position fixing device is provided, the unconscious movement of arms is avoided, the phenomena of influence on blood supply of skin flaps and vein backflow which are caused by the folding and torsion of skin flap pedicles due to the movement of the arms are eliminated, and the union of wounds is facilitated. The upper limb abdomen pedicled skin flap fixator has an adjustable height and angle and a stable and firm structure, is high in adaptability and generality, and can be used safely and reliably.

The invention relates to the soybean plantation, especially the replantation; the technical project of the invention to replant soybean is as following: cultivate the soybean seedlings for precocity, winter resistance, resisting drought and childbearing period about 84 days, and move them into farmland to ramify from rap roots and lateral roots and sizing, plant the second ones in the middle , and they can go on growing after the harvest of the first season ones. In Heilongjiang district, after the first season soybean, plant the second season soybean in the middle of the first one, and they can go on growing after the harvest of the first season ones. In Liaoning Province, if planted after the first ones have been harvested or before the mature, the second season soybean will have the fatness as follows: restisting high temperature, resisting drought, resisting ventitation, and prematurity and developed caulis. It can survive well in barren and ramify prosperously. This soybean grows flourish and mature in short time. The procreating period is 84 days, and all the active accumulated temperature is 1700 centigrade degrees. The method above can be used in south areas to harvest 3 times.

The invention discloses a thumb replantation board. The thumb replantation board comprises a square box and fixing rods, wherein the fixing rods are distributed on two long lateral sides and one short side of the square box; an upper arc-shaped slope groove is formed in the upper surface corresponding to the other short side with the fixed rod; the upper arc-shaped slope groove matches with an arc shape when the root of a palm is horizontally fixed; a lower arc-shaped slope groove is formed in the lower surface of the square box which is corresponding to the position of the upper arc-shaped slope groove. By adopting the above manner, 2-5 fingers can be well fixed; a fixing rubber band is easy and simple to buckle; after fixation, doctors can well perform an operation, thus survival rate of a replanted thumb is improved; the thumb replantation board is easy to clean, simple in structure, convenient to use and applicable to large-scale production.

The invention relates to a human amputated limb protection box based on non-contact sensing control perfusion technology, belongs to the field of medical auxiliary equipment, and provides a protectionbox which has reasonable structural design, can replace residual waste caused by ischemic injury, can be added with appropriate drugs for targeted treatment at the same time, can maintain the activity of isolated amputated limbs, further improves the replantation success rate and survival rate, and reduces the occurrence of limb disability. A condenser tube is arranged at the bottom of the protection box, a placing plate is arranged at the upper end of the condenser tube, a mounting plate is arranged at the middle end of the protection box, water is poured into the lower end of the mounting plate, a storage box, an oxygen cylinder and a medical peristaltic pump are arranged at the upper end of the mounting plate, a perfusion liquid storage box and a perfusion liquid buffer pool are arranged at the upper end of the placing plate, and then all devices are connected with each other through pipelines to form a perfusion pipeline, and nutrients required for maintaining the activity of theamputated limbs are supplied through the circulation flow of a perfusion liquid.

The invention relates to the field of three-dimensional printing preparation of nucleus pulposus replantation bodies, in particular to a method of preparing a nucleus pulposus replantation body in combination with a three-dimensional printing technology. According to the method of preparing the nucleus pulposus replantation body in combination with the three-dimensional printing technology, a polycaprolactone elastomer support is prepared by three-dimensional printing to serve as a mechanical support material of a nucleus pulposus implant; nucleus pulposus waste is treated to form a compositesupport with a polycaprolactone elastomer; and the support is good in mechanical property and biocompatibility, and contains a nucleus pulposus extracellular matrix and nucleus pulposus cells. The method of preparing the nucleus pulposus replantation body through the three-dimensional printing to treating degeneration of the lumbar intervertebral disc can obtain a series of polycaprolactone elastomer support products with different specifications via printing; the products are dry solids, and are easy to store and long in storage life; and at the same time, the nucleus pulposus replantation body can be replanted after immediate treatment intraoperatively by a replantation method after compositing of the nucleus pulposus waste and the polycaprolactone elastomer support.

The invention discloses an adipose-derived stem cell-biological material compound cryopreserved liquid which comprises 0.2-0.8mol / L of trehalose and 10-40% of hemopoietin. The invention also discloses a processing method of an adipose-derived stem cell-demineralized bone compound, and the cell survival rate and the vigor of the adipose-derived stem cell-demineralized bone compound processed by adopting the method are proved to be maintained to be at a relatively high level by detection; furthermore, animal in vivo replantation experimental results prove that the adipose-derived stem cell-demineralized bone compound cryopreserved by adopting the method still has the good repair effect, and thus a foundation for clinical application is established.

The invention relates to an external traditional Chinese medicine composition capable of preventing absorption of autogenous skull bone flaps and promoting healing of skull bone. The external traditional Chinese medicine composition is characterized by being prepared from the following components of 400g of radix pseudostellariae, 50g of borneol, 200g of dragon's blood, 400g of radix clematidis, 200g of radix angelicae dahurioae, 400g of rhizoma cibotii, 200g of rhizoma curculiginis, and 400g of herba taxilli. A preparation method comprises the following steps of preparation of a decoction medicine liquor: decocting the medicines twice, mixing the two times of medicine liquors, concentrating to 500ml, and loading the medicine liquor into a sterile tank for storing; preparation of an methylalcohol medicine liquor: drying 50g of borneol and 200g of dragon's blood by microwaves, crushing, screening in a 80-mesh way, and dissolving into 500ml of 75% ethyl alcohol. The external traditionalChinese medicine composition has the advantages that the materials are easy to obtain, and the preparation is easy; the traditional Chinese medicine formula is jointly used, and the synergistic function is realized; the cost of the use method is low, the economic effect is realized, the poison and harm are avoided, and the use effect is obvious; the external traditional Chinese medicine composition is mainly used for preventing the absorption of autogenous skull bone flaps and promoting the healing of skull bone after the replantation operation of the autogenous skull bone flaps.

The invention discloses a formula and a boiling method for a traditional Chinese medicine having repairing efficacy after replantation of a severed finger. In the formula, draconis resin promotes blood circulation and relieves pain, removes stasis to stop bleeding, and regenerates tissue to heal wond; radix notoginseng dissipates blood stasis to stop bleeding, and dispersing swelling and settlingpain; doubleteeth pubescent angelica roots dispells wind and eliminates dampness, and relieves pain of arthralgia aggravated by cold; safflowers and Sichuan lovage rhizome have the effect of promotingblood circulation to remove blood stasis; frankincense and myrrh are together utilized to promote blood circulation, promote qi and relieve pain, and disperse swelling and engender flesh; incised notopterygium rhizome and roots disperse exterior cold, expel wind-damp, are beneficial to arthrosis, and relieve pain; dahurian angelica roots dispel wind, dry dampness, disperse swelling, and relieve pain; Chinese angelica has the effect of tonifying qi and replenishing blood; fortune's drynaria rhizome and himalayan teasel roots have the effect of reunion of fractured tendon and bone; traditionalChinese medicines such as puerarin and rose-boots having the effects of promoting blood circulation to remove blood stasis, benefiting qi for activating blood circulation, promoting qi circulation andremoving obstruction in the collateral(s), and the like may have an effect of promoting blood vessel regeneration; and aloe has antibacterial and anti-inflammatory effects, treats tissue damage, protects skin, and can stimulate growth. Through the effects of setting a bone and replenishing the bone, promoting the blood vessel regeneration, stopping the blood and engendering the flesh, promoting the blood circulation and being beneficial to the arthrosis, the traditional Chinese medicine has a remarkable curative effect on repair of replantation of severed fingers.

The invention discloses a toe replantation plate which comprises a retaining block and two fixed blocks fixed at the two ends of the retaining block. The retaining block and the fixed blocks are each shaped like a rectangle, the retaining block is flush with the lower surfaces of the fixed blocks, and the height of the retaining block is a half to two thirds of that of the fixed blocks; the fixed blocks are arranged symmetrically, and the retaining block is located at the ends of the fixed blocks and is perpendicular to the fixed blocks. In this way, 90-degree position fixation of the ankle can be effectively maintained, the foot with problems is limited to fall left and right and move back and forth, and the broken toe can conduct turning operation conveniently. Meanwhile, a platform can be provided for containing the forearm and elbow of a surgeon.

The invention discloses a vertebral plate replantation fixing device capable of grafting bones. The vertebral plate replantation fixing device comprises a vertebral plate fixing piece, a first fastening nail and a second fastening nail, the vertebral plate fixing piece comprises a fixing part and a bony contact part; one end of the bony contact part is fixedly connected with the fixing part; the bony contact part is provided with a first contact surface and a second contact surface, the first contact surface is used for being in contact with a residual vertebral plate, the second contact surface is used for being in contact with a vertebral plate to be replanted, and the first contact surface and the second contact surface are oppositely arranged; at least one bone grafting through hole is formed in the bony contact part in a penetrating manner; the first fastening nail and the second fastening nail are detachably connected with the fixing part. The first fastening nail and the second fastening nail are oppositely located on the two sides of the bony contact part. The vertebral plate can be obviously lifted, the volume of the vertebral canal can be enlarged, locking and fixing are stable, bone grafting is conducted through the bone grafting through hole, later-stage bone ingrowth is achieved, then strong connection of the vertebral plate to be replanted and the residual vertebral plate is achieved, irritation to surrounding soft tissue of the vertebral plate is small, and bone damage is reduced.

The invention discloses a device and a method for evaluating blood supply after severed limb replantation and free flap surgery. The device comprises a photoelectric volume pulse wave signal acquisition device, a fluorescence image acquisition device and a workstation. Compared with the prior art, the method has the advantages that the ICG pigment concentration spectrum extracted from the PPG signal is used as the normal metabolism curve of the ICG pigment in the human body, the difference between the ICG metabolism curve at each pixel point acquired by the ICGA and the normal ICG metabolism curve in the human body is compared, the severed finger (limb) replantation and free flap postoperative blood supply condition is evaluated, and the severed finger (limb) replantation and free flap postoperative blood supply condition is evaluated. According to the method, the influence of adverse factors on the accuracy of ICGA evaluation of flap blood supply results is avoided or reduced, severed finger (limb) replantation and free flap postoperative blood supply evaluation parameters are directly generated and displayed in an imagination mode, intuition and objectivity are achieved, and the subjectivity that surgeons evaluate severed finger (limb) replantation and free flap postoperative blood supply conditions according to clinical experience is avoided.

The invention discloses a radix semiaquilegiae tissue culture and rapid propagation method. Radix semiaquilegiae belongs to a dried root of ranunculaceae plant, and is mainly produced in Jiangsu, Hubei and Hunan. Radix semiaquilegiae is harvested in early summer, a root tuber is dug out, soil is washed out, and the root tuber is dried in the sun or dried beside or over a fire. Radix semiaquilegiaehas tiny odor, and is sweet and slightly bitter in taste. Radix semiaquilegiae has the effect of clearing away heat and toxic materials, promoting the subsidence of swelling and dispersing accumulation of pathogen. Radix semiaquilegiae has mild toxicity, is used for scrofula, swollen welling-abscess and clove sores, traumatic injury and venomous snake bites. Seedling raising of radix semiaquilegiae is mainly performed through traditional methods, such as stem planting, grafting and sowing, and the radix semiaquilegiae has the disadvantages that the seedling raising cost is high, nursery stockis not good in growth vigor and uneven, and improved variety potential cannot be brought into full play. The method has the advantages that an excellent clone spray is taken as explant, radix semiaquilegiae in vitro replantation plant is obtained through steps of explant disinfection, induced culture, cluster bud multiplication, adventitious rooting and acclimatization and transplant, a radix semiaquilegiae tissue culture and rapid propagation technology is established, excellent characters of radix semiaquilegiae clone female parent can be kept, and the method is beneficial to scale production of radix semiaquilegiae excellent clone nursery stock.

The present invention provides a knee joint tibial plateau prosthesis, which includes a tibial plateau prosthesis and a tibial tubercle prosthesis, wherein the tibial tubercle prosthesis is arranged at the tibial tubercle of the tibial plateau prosthesis, and the tibial tubercle prosthesis is externally convex. Because the part of the tibial plateau prosthesis is a trabecular bone structure, there is a concave setting on the trabecular bone structure, which is used for placing the tibial tubercle from the insertion point of the patellar tendon to connect the patellar tendon to free the lower bone fragment. A solid beam with smooth surface is arranged across the notch of the beam, the beam and the bone trabecular structure are integrally arranged, and the prostheses on both sides of the beam are symmetrically provided with patellar ligament suture holes. Wherein, the placement groove can provide a replantation space for the bone fragment below the patellar tendon to be freed, and ensure that the patellar ligament is completely implanted on the tibial plateau prosthesis. The stress is dispersed, and the beam and the trabecular bone structure are integrally arranged, which further prevents the beam from loosening due to the long-term expansion and contraction of the patellar ligament.