The invention discloses a radix semiaquilegiae
tissue culture and rapid propagation method. Radix semiaquilegiae belongs to a dried root of ranunculaceae
plant, and is mainly produced in Jiangsu, Hubei and Hunan. Radix semiaquilegiae is harvested in early summer, a root tuber is dug out, soil is washed out, and the root tuber is dried in the sun or dried beside or over a fire. Radix semiaquilegiaehas tiny
odor, and is sweet and slightly bitter in taste. Radix semiaquilegiae has the effect of clearing away heat and toxic materials, promoting the
subsidence of swelling and dispersing accumulation of
pathogen. Radix semiaquilegiae has mild
toxicity, is used for scrofula, swollen welling-
abscess and clove sores,
traumatic injury and
venomous snake bites.
Seedling raising of radix semiaquilegiae is mainly performed through traditional methods, such as stem planting,
grafting and
sowing, and the radix semiaquilegiae has the disadvantages that the
seedling raising cost is high, nursery stockis not good in growth vigor and uneven, and improved variety potential cannot be brought into full play. The method has the advantages that an excellent clone spray is taken as explant, radix semiaquilegiae
in vitro replantation plant is obtained through steps of explant disinfection, induced culture, cluster
bud multiplication, adventitious rooting and
acclimatization and transplant, a radix semiaquilegiae
tissue culture and rapid propagation technology is established, excellent characters of radix semiaquilegiae clone female parent can be kept, and the method is beneficial to scale production of radix semiaquilegiae excellent clone nursery stock.