Preparation method of rapidly decellularized unifoliate liver biological scaffold

Abstract

The invention discloses a preparation method of a rapidly decellularized unifoliate liver biological scaffold. An ionic detergent sodium dodecyl sulfonate (SDS) with the problem that thrombus is easy to form after tissue transplantation and the problem of low replantation rate of decellularized tissues in a composite cellularization process is not used; a decellularized left outer-lobe liver biological scaffold is prepared by a weakly alkaline Triton solution perfusion method; due to the addition of NaOH in the Triton solution, the neutral Triton solution is in alkalescence; the cell lysis removal effect is enhanced; the time required for removing cells is shortened; and the optimal concentration and dosage of the NaOH and the Triton solution are adopted, so that the decellularization effect is the best.

Description

technical field [0001] The invention relates to the field of organ tissue decellularization, in particular to a method for preparing a rapid single-lobe liver decellularized biological scaffold. Background technique [0002] Extracellular matrix (extracellular matrix, ECM) is a substance packed between cells, and its components include water, electrolytes, and a small amount of liquid phase components, mainly collagen, mucin, and proteoglycans, which together constitute the microenvironment for cell growth . The local microenvironment of hepatocytes is a variety of cytokines secreted by the extracellular matrix and stromal cells, including hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), transforming growth factor (TGF-β), and platelet-derived growth factor (PDGF). Among them, ECM not only provides a three-dimensional spatial structure for the growth of intrahepatic cells, but a...

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Problems solved by technology

[0003] Since 2010, foreign scholars have successfully prepared whole liver decellularized bioscaffolds by perfusion with different detergents[4,7-11], but there is no uniform standard for the perfusion pathway and protocol, which will affect the preparation quality and quality of decellularized liver bioscaffolds. serious impact on stability

However, as a commonly used detergent, the residue of SDS will lead to easy formation of thrombus after tissue transplantation, and the low replantation rate of decellularized tissue during recellularization[13]

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