The invention discloses a ventilated and heat-retaining edible fungus cultivation box, which comprises a box body, a cultivation rack, a groove, a buckle and a sealing ring. Ventilation fans are inlaid on the rear side wall of the box body, and ventilation fans are provided with Heating device, the bottom of the heating device is connected with the connecting pipe, and the connecting pipe is provided with an air outlet, the cultivation frame is arranged in the box, and the inner body wall of the box is provided with a thermal insulation layer, and the concave The groove is arranged on the top of the box body, and the front side of the box body is provided with a thermometer, the buckle is arranged above the outer side wall of the box body, and a box cover is provided above the box body, and the side of the box cover is provided with block, and a glass viewing port is provided in the middle of the case cover, and the sealing ring is provided on the bottom surface of the case cover. The ventilated and heat-retaining edible fungus cultivation box is provided with a heating device, which can heat and blow cold air into the box, so that the box can have a suitable temperature for cultivating edible fungi.