The invention discloses novel acoustic tweezers based on an artificial structure flat plate. The technology-dependent operating system comprises a three-dimensional translation device, an ultrasonic transmitting device for radiating ultrasonic waves, an artificial structure flat plate and an objective table, the objective table is used for placing particles to be controlled, the artificial structure flat plate has a special geometric design and is used for modulating a sound field to form focused petal sound beams with various special configurations and capturing particles with different sizes, and the three-dimensional translation device is used for moving the focused petal sound beams generated by the artificial structure flat plate, so that free transmission and transportation of the particles are realized. According to the novel acoustic tweezers, the artificial structure flat plate is mainly used for modulating a sound field to generate various focusing petal acoustic beams, particles with different sizes are captured in the central area of an acoustic beam by using the acoustic radiation force characteristic of the acoustic beam, and the transmission and transportation of theparticles are realized by moving the focusing petal acoustic beam, so that the technology has important research value in the practical application aspects of material preparation, cell separation, drug transportation, bioengineering and the like.