Radix semiaquilegiae tissue culture and rapid propagation method

Abstract

The invention discloses a radix semiaquilegiae tissue culture and rapid propagation method. Radix semiaquilegiae belongs to a dried root of ranunculaceae plant, and is mainly produced in Jiangsu, Hubei and Hunan. Radix semiaquilegiae is harvested in early summer, a root tuber is dug out, soil is washed out, and the root tuber is dried in the sun or dried beside or over a fire. Radix semiaquilegiaehas tiny odor, and is sweet and slightly bitter in taste. Radix semiaquilegiae has the effect of clearing away heat and toxic materials, promoting the subsidence of swelling and dispersing accumulation of pathogen. Radix semiaquilegiae has mild toxicity, is used for scrofula, swollen welling-abscess and clove sores, traumatic injury and venomous snake bites. Seedling raising of radix semiaquilegiae is mainly performed through traditional methods, such as stem planting, grafting and sowing, and the radix semiaquilegiae has the disadvantages that the seedling raising cost is high, nursery stockis not good in growth vigor and uneven, and improved variety potential cannot be brought into full play. The method has the advantages that an excellent clone spray is taken as explant, radix semiaquilegiae in vitro replantation plant is obtained through steps of explant disinfection, induced culture, cluster bud multiplication, adventitious rooting and acclimatization and transplant, a radix semiaquilegiae tissue culture and rapid propagation technology is established, excellent characters of radix semiaquilegiae clone female parent can be kept, and the method is beneficial to scale production of radix semiaquilegiae excellent clone nursery stock.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of geranium seeds. Background technique [0002] Tiankuizi is the dry root tuber of Ranunculaceae plants, mainly produced in Jiangsu, Hubei, Hunan and other places. Harvest in early summer, dig out the roots, wash the soil, and dry or dry. Slight gas, sweet and slightly bitter. With heat-clearing and toxic substances removing, reducing swelling and dissipating stagnation. It is slightly poisonous. For scrofula, carbuncle furuncle, bruises, poisonous snake bites. In recent years, with the advancement of the reform of the collective forest right system and the rise in the price of commercial materials, forest farmers have become more active in managing geranium seeds, and the management level and awareness of improved varieties of geranium seeds have been continuously improved, and the demand for...

AI Technical Summary

Problems solved by technology

At present, the seedlings of Tiankuizi mainly adopt traditional methods such as cutting, grafting and sowing, which have the disadvantages of high seedling cost, poor growth of seedlings, unevenness, and failure to fully exert the potential of improved seeds, making the current high-quality and strong seedlings of Tiankuizi The supply cannot meet the market demand