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Quick cultivation method for tissue culture of hematoxylon

A tissue culture fast propagation and hematoxylin technology, applied in the field of plant tissue culture, can solve the problems of uneven, high cost of raising seedlings, poor growth of seedlings, etc.

Inactive Publication Date: 2019-01-25
林登淞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Sappu mainly adopts traditional methods such as cutting, grafting and sowing to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully exert the potential of improved seeds. Still unable to meet the needs of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Collection and disinfection of explants: the plump twigs of the sprouting state with consistent morphological characteristics at the base of the stump of hematoxylin were used as explants, rinsed under running water for 5h, soaked in 3% detergent solution for 8min, and used soft hair Brush 3% detergent powder solution to gently scrub the material, then rinse with tap water in the form of dripping water for 63 minutes, rinse with distilled water 6 times, soak in 75% ethanol solution in an ultra-clean workbench for 23s, rinse with sterile water 8 times, and then use Sterilize with a 0.3% mercury liter solution of 0.01% Tween-20 for 8 minutes, rinse with sterile water for 6 times and absorb the moisture on the surface with sterile filter paper for later use.

[0014] (2) Induction culture: Cut the tender branches treated in step (1) into about 1.8cm stems and inoculate them into the induction medium for cluster bud induction culture. After inoculation, it was placed in th...

Embodiment 2

[0019] (1) Collection and disinfection of explants: The plump twigs in the sprouting state at the base of the stump of hematoxylin were used as explants, rinsed under running water for 6 hours, soaked in 3% washing powder solution for 11 minutes, and used with soft hair Brush the 4% detergent powder solution gently to scrub the material, then rinse with tap water in the form of dripping for 73 minutes, rinse with distilled water 7 times, soak in 78% ethanol solution in the ultra-clean workbench for 33s, rinse with sterile water 8 times, and then use Sterilize with 0.3% mercury liters solution of 0.01% Tween-20 for 10 minutes, rinse with sterile water for 8 times and absorb the moisture on the surface with sterile filter paper for later use.

[0020] (2) Induction culture: Cut the tender branches treated in step (1) into about 1.8cm stems and inoculate them into the induction medium for cluster bud induction culture. After inoculation, it was placed in the light for 16 hours a day...

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PUM

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Abstract

The invention discloses a quick cultivation method for tissue culture of hematoxylon. The defects are overcome that in the prior art, traditional modes such as cuttage, grafting and sowing are mainlyadopted for seedling raising, the seedling raising cost is high, the growth vigor of seedlings is bad and uneven, and the fine breed potential is not sufficiently achieved. According to the method, with good clone tender branches as explants, through the processes such as explant sterilization, induction cultivation, cluster-bud multiplication, adventitious root occurrence and acclimatization andtransplantation, in-vitro replantation of hematoxylon is achieved, the quick cultivation technical system for tissue culture of hematoxylon is established, the good characters of a clone female parentof hematoxylon can be kept, and the method is beneficial to large-scale production of good clone seedlings of hematoxylin.

Description

Technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, and specifically relates to a method for hematoxylin tissue culture and rapid propagation. Background technique [0002] Hematoxylin is a legume plant, and its dry heartwood is used. Generally, the tree is cut down from May to July, the rough bark and sapwood are removed, and the yellow-red or red-brown heartwood is taken and dried. Odorless, slightly astringent. It has the function of removing blood stasis, reducing swelling and pain. Used for amenorrhea, postpartum stasis, abdominal pain, chest and abdomen pain, trauma and fracture pain. In recent years, with the advancement of the collective forest tenure system reform and the increase in commodity timber prices, forest farmers have become more enthusiastic about managing hematoxylin. The management level of hematoxylin and the awareness of improved varieties have continued to increase. The demand for improved he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 林登淞
Owner 林登淞
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