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Method for constructing tissue engineered cartilage by using PCL kernel-containing material

A tissue engineering and core technology, applied in medical science, prosthesis, etc., can solve the problems of difficult to overcome the original form of subcutaneous tension, insufficient mechanical properties of cartilage, and interference with cartilage regeneration.

Inactive Publication Date: 2014-07-30
SHANGHAI TISSUE ENG LIFE SCI
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  • Abstract
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Problems solved by technology

[0003] However, due to the lack of mechanical properties of the cartilage constructed in vitro in the prior art, it is difficult to overcome the subcutaneous tension and maintain the original shape after being implanted in the animal body
Although titanium mesh support-assisted transplantation can solve the problem of shape maintenance in nude mice, it can cause foreign body reactions and interfere with cartilage regeneration in large animals

Method used

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Embodiment Construction

[0033] Hereinafter, the above-mentioned and other technical features and advantages of the present invention will be described in more detail.

[0034] The specific process of the method for constructing tissue engineered cartilage using the PCL core material in the present invention is as follows:

[0035] Step a, making the PLC core; the PCL core is a grid-shaped porous scaffold made of PCL granular material by thermal fusion deposition rapid prototyping technology. In this embodiment, the grid spacing is 2 mm, and the layer height is 0.1 mm. Cut the grid-shaped PCL to a suitable size, place it in the auricle mold and fix it under pressure. After heating at 55-70°C for about 5-10 minutes, pressurize rapidly at a pressure of 200N-500N; after cooling down to room temperature during the pressurization process, take out the auricle-shaped PCL core and trim the excess material.

[0036] Step b, making a PGA / PLA composite ear bracket with a PCL core;

[0037] Step b1, tearing th...

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Abstract

The invention relates to a method for constructing tissue engineered cartilage by using a PCL kernel-containing material. The method comprises the steps of a, making a PLC kernel; b, making a PLC kernel-containing PGA / PLA compound ear bracket; c, making a PGA / PLA compound ear bracket; d, culturing cartilage cells; e, constructing cartilage auricle bracket; and f, nude mouse subcutaneous replantation of in-vitro constructed auricle: respectively carrying out nude mouse subcutaneous implantation on the cartilage cells and an ear bracket compound after culturing in vitro for a week. According to the method, the tissue engineered cartilage can be constructed by using the PLC kernel-containing material, so that enough strength is provided to resist the tension caused by the subcutaneous implantation and then the original complicated and precise shape is maintained.

Description

technical field [0001] The invention relates to the field of biological tissues, in particular to a method for constructing tissue engineered cartilage using a core material with PCL. Background technique [0002] Microtia is the most common congenital deformity after cleft lip and palate. The traditional treatment is mainly based on autologous costal cartilage transplantation, which causes great damage to the donor site. Theoretically speaking, tissue engineering technology is obviously superior to the traditional treatment mode in at least the following three aspects: (1) It causes less damage to the patient, and a small amount of cells can be expanded to build a large piece of cartilage; (2) The constructed cartilage has functions and is truly Structural and functional reconstruction in the sense; (3) It can be shaped precisely, which is more in line with the needs of clinical applications. [0003] However, the cartilage constructed in vitro in the prior art has insuffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/54
Inventor 周广东刘豫曹谊林殷宗琦
Owner SHANGHAI TISSUE ENG LIFE SCI
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