94 results about "Tissue engineered cartilage" patented technology

The invention discloses a three-dimensional porous cartilage extracellular matrix sponge scaffold made of natural cartilage, which can compound cells further to construct tissue engineered cartilage, and can be used for clinically repairing cartilage defects. In the invention, conditions which can fully perform cell extraction and form a porous scaffold matrix are provided by processing cartilage into cartilage microfilaments, and then the cell extraction and solidification and / or strengthening treatment are performed to obtain the three-dimensional porous cartilage extracellular matrix sponge scaffold which is completely decellurized. The antigenicity and cell components are removed in the natural cartilage, and an extracellular matrix component of the cartilage is retained to obtain the scaffold with appropriate pore diameter and porosity, suitable degradation rate, good biocompatibility and certain biomechanical strength. The scaffold has the advantages of broad material sources, low cost, simple and feasible preparation technology, and good repetitiveness; and the scaffold can be widely applied in the field of tissue engineering and has good clinical application prospect.

Cartilage has been constructed using biodegradable electrospun polymeric scaffolds seeded with chondrocytes or adult mesenchymal stem cells. More particularly engineered cartilage has been prepared where the cartilage has a biodegradable and biocompatible nanofibrous polymer support prepared by electrospinning and a plurality of chondocytes or mesenchymal stem cells dispersed in the pores of the support. The tissue engineered cartilages of the invention possess compressive strength properties similar to natural cartilage. Methods of preparing engineered tissues, including tissue engineered cartilages, are provided in which an electrospun nanofibrous polymer support is provided, the support is treated with a cell solution and the polymer-cell mixture cultured in a rotating bioreactor to generate the cartilage. The invention provides for the use of the tissue engineered cartilages in the treatment of cartilage degenerative diseases, reconstructive surgery, and cosmetic surgery.

The invention discloses a method for in vitro establishment of special-shaped tissue engineering scaffolds, comprising steps as follows: (1) applying computer aided design to establish a digital model for special-shaped tissues or organs; (2) guiding the digital model acquired in the step (1) into a rapid prototyping machine for processing, to shape a solid female die for the special-shaped tissues; (3) adding biodegradable materials acceptable by the medical science into the solid female die gained in the step (2), to acquire a special-shaped tissue engineering scaffold. The special shapes are chosen according to the shapes of auricle, external nose, nose prosthesis, chin prosthesis and other human issues or organs. The special-shaped tissue engineering scaffold can be effectively compounded with the chondrocytes to finally form special-shaped tissue engineering cartilages. The invention also provides a detailed method of preparing tissue engineering auricle and external nose scaffolds and application thereof.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The present invention relates to one kind of biological rack material for articular cartilage and its preparation process. The porous rack material for articular cartilage is prepared through mixing natural cartilage matrix component with the cell, antigen and type I and type X collagen eliminated and type II gollagen, and serial physical and chemical treatments with sodium chloride crystal grain as pore forming agent. The rack material has the components the same as those of natural cartilage, high biomechanical strength, high porosity, proper degradation speed, use convenience and no influence on the body's and local immunity. The present invention may be used in the clinical repair of articular cartilage defect and in vivo and in vitro construction of tissue engineering cartilage.

The invention belongs to the field of cartilage tissue engineering. The invention discloses a tissue engineering cartilage composite scaffold, which is constructed based on an extracellular matrix-derived cartilage micro-tissue, and a preparation method of the composite scaffold. Specifically, a cartilage extracellular matrix-derived micro-carrier is prepared from fresh cartilage through such processes as wet pulverization, sieving, decellularizing and the like; and the prepared micro-carrier can be used as a seed cell amplification vector. By virtue of a bioreactor, cartilage cells are rapidly amplified by the micro-carrier and the phenotype of the cartilage cells is kept; and meanwhile, the induced differentiation of stem cells towards the cartilage is also promoted and the cartilage micro-tissue is formed. By filling the cartilage micro-tissue in pores of a three-dimensional porous scaffold which is good in mechanical performance, the tissue engineering cartilage composite scaffold is constructed. The composite scaffold is good in mechanical performance, rich in natural cartilage extracellular matrix components and conducive to the induced differentiation of the stem cells towards the cartilage, and the composite scaffold is capable of providing a good microenvironment for the growth of seed cells and is expected to accelerate the in vivo construction speed of the tissue engineering cartilage.

The invention relates to a tissue engineering cartilage composite stent and a preparation method. The composite stent comprises a stent body of a pore structure and hydrogel; the stent body is prepared from a medical macromolecule material by rapid prototyping; the hydrogel is compounded in the pore structure of the stent body and the surface of the stent body, and the hydrogel is mixed with bone marrow mesenchymal stem cells. By compounding the stent body prepared from the hydrogel and the medical macromolecule material, the prepared tissue engineering cartilage composite stent not only has good mechanical strength of the medical macromolecule material, but also provides a good microenvironment for material interchange of the bone marrow mesenchymal stem cells in the stent and on the surface of the stent and differentiation to chondrocytes by keeping a high water content through the hydrogel.

The invention relates to a method for obtaining tissue engineering cartilage through directionally inducing bone-marrow mesenchymal stem cells (BMSCs). At present, a large amount of exogenous growth factors and culture in vitro are needed before culturing the BMSCs and the cartilage cells in vivo to promote the differentiation of the BMSCs towards the cartilage cells. The method comprises the following steps of: mixing the sub-cultured P3-generation BMSCs, the cartilage cells and a large extracellular matrix aggregate (cell brick), resuspending the mixture into a platelet rich plasma to prepare a cell compound of the sub-cultured P3-generation BMSCs, the cell brick and the platelet rich plasma, injecting the compound into the skin on the back of a nude mice when the compound condenses gradually, and culturing to obtain the new tissue engineering cartilage. The completely biological and injectable tissue engineering cartilage is obtained on the basis of coculturing the BMSCs and the cartilage cells. The differentiation of the BMSCs towards the cartilage cells can be kept stably, the hypertrophy of the tissue engineering cartilage in vivo can be inhibited effectively, and the defects in the background art can be overcome.

The invention discloses a cartilage extracellular matrix and silk fibroin composite orientation cartilage support and a preparation method thereof and belongs to the biological tissue engineering technology. According to the cartilage extracellular matrix and silk fibroin composite orientation cartilage support and the preparation method of the cartilage support, cartilage extracellular matrixes are prepared through articular cartilage of people, pigs, cows or sheep, silk fibroin is prepared through mulberry silk, after the cartilage extracellular matrixes and the silk fibroin are evenly mixed according to a specific proportion, directed crystallization is performed under a certain temperature, ultraviolet cross linking is firstly performed after freezing and drying, and then cross linking of carbodiimide and N-hydroxysuccinimide or glutaraldehyde and genipin is performed so that the cartilage extracellular matrix and silk fibroin composite orientation cartilage support can be prepared. Due to the design, biocompatibility is good, and immunological rejective reaction is avoided; a composition structure and mechanical properties are similar to those of cartilage extracellular matrixes of people; material sources are wide, cost is low, the preparation technology is simple, the cartilage extracellular matrix and silk fibroin composite orientation cartilage support can be used for constructing tissue engineering cartilage and repairing cartilage degradation, and clinical application prospects are good.

The invention relates to an injectable cartilage repairing hydrogel and a preparation method thereof, which belong to the technical field of biological materials. The injectable cartilage repairing hydrogel of the present invention comprises modified hyaluronic acid, modified gelatin, a platelet rich plasma, and an ultraviolet photoinitiator. The materials of the cartilage repairing hydrogel of the invention have good biocompatibility and injectability, the platelet-rich plasma can achieve a variety of growth factors, such as vascular endothelial growth factors, transforming growth factors, and the like, can be used in tissue engineering cartilage repairing.

The invention discloses a preparation method of a composite hydrogel tissue engineer cartilage repairing scaffold with biological activity. The preparation method comprises the steps of: preparing a paraffin model of the cartilage repairing scaffold; by taking the paraffin model as a template, selecting and fermenting-cultivating a bacterial strain capable of secreting bacterial cellulose; removing paraffin from a fermentation product, and purifying, dewatering and immersing the fermentation product, thus obtaining a bacterial cellulose hydrogel cartilage repairing scaffold containing 30-50% of CaC12 aqueous solution by weight; dissolving polyvinyl alcohol in an Na2HPO4 aqueous solution, thus obtaining a mixed solution A; enabling the mixed solution A to enter the bacterial cellulose hydrogel cartilage repairing scaffold by virtue of an immersion method to obtain a product; freezing-unfreezing the obtained product for multiple times, and irradiating the product by gamma rays, thus obtaining the composite hydrogel tissue engineer cartilage repairing scaffold with biological activity. Composite hydrogel prepared by the method has an appropriate appearance, a porous structure, an excellent mechanical property and excellent biological activity and can be used as a tissue engineer scaffold material for repairing cartilage tissue.

The invention belongs to tissue engineering field. It makes self body mesenchymal stem cell as seed cell and porous biological ceramics as bracket material. Adding proliferating agent and differentiating agent when cultivating the complex in vitro, transplanting and restoring articular cartilage injury after cultivating in vitro for a period of time The invention is characterized in that it constructs tissue engineering cartilage by employing marrow mesenchymal stem cell and porous biological ceramics for restoring articular cartilage injury. It has an preferably clinical practice prospect.

The invention discloses an amino acid sequence of bone marrow mesenchymal stem cell affinity peptide, a screening method and an application and belongs to the field of bio-pharmaceuticals. The screening method comprises the following steps: utilizing a phage display process to respectively perform negative screening of human fibroblasts and positive screening of a bone marrow mesenchymal stem cell; after screening, extracting the bone marrow mesenchymal stem cell, and then decomposing the cell; extracting a phage segment which has specific affinity to the bone marrow mesenchymal stem cell; and sequencing the phage segment, thereby acquiring a peptide segment which has high affinity to the bone marrow mesenchymal stem cell. The amino acid sequence of bone marrow mesenchymal stem cell affinity peptide can be used for promoting the specific affinity of biological material to the bone marrow mesenchymal stem cell and has profound significance and wide application prospect in the field of repairing tissue engineered cartilages.

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a tissue engineered bone cartilage frame and a preparation method thereof. Particularly, marrow removal and degreasing are carried out on allogeneic or xenogeneic cancellous bones to obtain cancellous bone columns with experimentally or clinically required diameters, and then a tissue engineered bonecartilage frame with a completely decalcified upper layer and a superficially decalcified lower layer is obtained via layered decalcification. The upper layer of the frame is used as the framework part of the tissue engineered cartilage frame or an engineering cartilage frame, and the lower layer thereof can be used as a bone forming frame, an engineering bone bracket and the fixed pivot of the engineered cartilage, and can be used for constructing an integrative tissue engineered bone cartilage or directly transplanted for the cartilage or bone cartilage repair. Compared with the existing tissue engineered bone cartilage frame, the invention has the advantages of integration (no interface mechanical defect), and good compatibility of a pore structure and the tissue, has better compression resisting, tensile resisting and shear resisting properties, and is convenient for the minimally invasive surgery operation under an arthroscope.

The invention discloses a tissue engineered cartilage in-vitro culture device. The tissue engineered cartilage in-vitro culture device comprises a machine frame. A cartilage culture mechanism is arranged on the machine frame. The tissue engineered cartilage in-vitro culture device further comprises a culture solution circulation mechanism used for conveying a culture solution to the cartilage culture mechanism, a dynamic static pressure exertion mechanism used for exerting dynamic static pressure on the cartilage culture mechanism, and a dynamic sealing mechanism used for controlling culture solution input and output of the culture solution circulation mechanism. By means of the tissue engineered cartilage in-vitro culture device, the static pressure frequency and magnitude of cartilage culture and the flowing situation of the culture solution are regulated, the environment of dynamic static pressure is simulated, and cartilage which is good in cell viability and good in cartilage form and has certain mechanical strength can be cultured.

The invention discloses a mechanical environment culture method for improving the proliferation activity of chondrocytes and maintaining phenotype of chondrocytes. The invention comprises the following steps: step 1, carrying out separation, culture and identification of chondrocytes; 2, inoculating the chondrocytes into a culture apparatus, loading the chondrocytes during the culture process, andculturing the chondrocyte in a mechanical environment so that the chondrocytes are mechanically stimulated. Under culture in mechanical environment, the proliferation of chondrocytes is improved, andco-cultured cells is promoted to have a good cartilage phenotype, which can quickly and effectively provide excellent seed cells for cartilage tissue engineering, and solve the clinical and practicalneeds of tissue engineering cartilage seed cells.

The invention discloses a tissue engineering cartilage framework material. The cartilage framework material provided by the invention sequentially comprises a surface layer, a middle layer and a calcification layer from the top down; the surface layer is composed of freeze-dry cartilage matrix and II-type collagen, and matrix fiber is in parallel distribution in the horizontal direction; the middle layer is composed of freeze-dry cartilage matrix, GAG, X-type collagen, II-type collagen and TGF-Beta, and matrix fiber is arranged longitudinally; and the calcification layer is composed of freeze-dry cartilage matrix, GAG, X-type collagen and sintered bovine bone power, and fiber is distributed in a three dimensional staggered manner. The prepared cartilage framework material has a cartilage repairing effect, is favorable to the integration of the framework material and subchondral bone after the framework material is planted, and promotes the regeneration of the subchondral bone; after cartilage cell compound, the framework material can be used for full-layer cartilage defect repairing, the regenerated cartilage tissue has the space structure and protein components, which are the same with those of natural cartilage; and the framework material is suitable for repairing cartilages in load bearing joints such as knee joints and the like, cartilage with higher regenerated compressive strength can be regenerated, and the abrasion performance and anti-pressure capability of the surface of the cartilage framework are enhanced.

The invention discloses a preparation method and application of a silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold. The method utilizes mulberry silk to prepare regenerated the silk fibroin, purifies industrial grade chitin, mixes a silk fibroin solution and a chitin solution, prepares a blended nanofiber membrane by an electrospinning technique, cross-links with ethanol to form a short fiber through a blended nanofiber, mixes the short fiber and the silk fibroin solution, conducts cross-linking by a crosslinking agent, and conducts freeze-forming to obtain a spongy cartilage bionic scaffold. In particular, the insertion of the nano short fiber increases the compressive strength and biocompatibility of the scaffold material. The method constructs the biomimetic scaffold with good biocompatibility, low immune rejection and similar composition with natural cartilage matrix, has wide material source, low cost and simple preparation process, can beused for constructing tissue engineering cartilage and repairing cartilage regression, and has good clinical application prospects.

The invention belongs to the field of tissue engineering, and discloses a preparation method of a nano-fiber scaffold for promoting cartilage regeneration to solve the problem that Nell-1 serving as anovel cartilage-derived growth factor can promote cartilage bone cell functions, but is easily inactivated in the process of being integrated into a tissue engineering cartilage scaffold. The preparation method includes the steps: firstly, preparing Nell-1 supported chitosan nano-particle solution and collagen / poly (L-lactic acid)-copolymer (epsilon-caprolactone) mixed solution; secondly, preparing a PLLA-CL / collagen nano-fiber mesh by coaxial electro-spinning and dynamic liquid collection technology; finally, preparing the collagen / HA mixed nano-fiber scaffold by collagen and hyaluronic acid(HA). The operation method is simple, the prepared nano-fiber scaffold is good in biological compatibility, biological activity of the growth factor can be protected, the release time of the growth factor is prolonged, and cartilage repair and regeneration can be promoted.

The invention relates to a method for constructing tissue engineered cartilage by using a PCL kernel-containing material. The method comprises the steps of a, making a PLC kernel; b, making a PLC kernel-containing PGA / PLA compound ear bracket; c, making a PGA / PLA compound ear bracket; d, culturing cartilage cells; e, constructing cartilage auricle bracket; and f, nude mouse subcutaneous replantation of in-vitro constructed auricle: respectively carrying out nude mouse subcutaneous implantation on the cartilage cells and an ear bracket compound after culturing in vitro for a week. According to the method, the tissue engineered cartilage can be constructed by using the PLC kernel-containing material, so that enough strength is provided to resist the tension caused by the subcutaneous implantation and then the original complicated and precise shape is maintained.

The invention discloses a tissue engineering cartilage graft. The tissue engineering cartilage graft contains human residual ear cartilage cells growing under a three-dimensional structure, and the human residual ear cartilage cells are passaged at the first, second, third, fourth, fifth, sixth, seventh or eighth passage. The invention also discloses an application of the human residual ear cartilage cells in the in-vitro construction of the cartilage graft.

The invention discloses a tissue engineering bone and cartilage complexed tissue, wherein the tissue engineering bone and tissue engineering cartilage are bonded together through recesses and projections in concave-convex relation arranged on the bonding interfaces, biological albumen gel is sprayed into the two tissue interface. The invention also discloses the method for constructing the complex tissue extracorporally. The disclosed tissue structure has the advantages of perfect histological structure, secure and convenient assembly and easy accessible raw materials.

The invention discloses a growth factor sustained release microsphere, a tissue engineering cartilage composite stent and a preparation method. The growth factor sustained release microsphere includesa polylactic acid-glycolic acid copolymer matrix material and recombinant human transforming growth factor beta 3 encapsulated in the matrix material, wherein the encapsulation amount of the recombinant human transforming growth factor beta 3 in the microsphere is 5 ng / mg to 200 ng / mg; the sustained release period of the microsphere is 28 days or above. The growth factor sustained release microsphere and the tissue engineering cartilage composite stent disclosed by the invention have higher regeneration and repair effects of defected cartilage tissues.

The invention relates to a tissue tissue-engineered cartilage graftimplant and a preparation method thereof and belongs to the technical field of induced differentiation carried out on bone marrow mesenchymal stem cells (BMSCs) by utilizing a bioactive inducing factor to form a cartilage cell chondroblast composite scaffold material and so as to construct a tissue tissue-engineered cartilage by utilizing a biological activity inducing factor in biomedicine tissue engineering. The tissue tissue-engineered cartilage graftimplant is prepared by adopting the method comprising the following steps: (1) preparing a Nano-HA / PLLA (hyaluronic aciddroxyapatite / poly left L-lactic acid) cartilage scaffold material; (2) carrying out coculture on BMSCs and the Nano-HA / PLLA cartilage scaffold material, and carrying out induced differentiation on BMSCs to form cartilage cells by adopting a cartilage formation inducing solution, so that the tissue tissue-engineered cartilage graftimplant is obtained. The tissue tissue-engineered cartilage graftimplant improves flexibility and biodegradability of the cartilage scaffold material, improves biomechanical property and is more beneficial to adhesion, growth and vascularization of bone cells; an animal experiment proves that the tissue tissue-engineered cartilage graftimplant has a good cartilage defect repairing function.

The invention discloses a tissue engineering cartilage stent and a method for preparing the same. The method includes a step A, extracting II-type collagen; a step B, preparing a primary CII-HA-CS-HAP three-dimensional cartilage stent by the aid of the II-type collagen, hyaluronic acid, chondroitin sulfate and hydroxyapatite; a step C, crosslinking the primary CII-HA-CS-HAP three-dimensional cartilage stent by the aid of an EDC / NHS system and freezing and drying the CII-HA-HAP three-dimensional cartilage stent to obtain the CII-HA-CS-HAP three-dimensional tissue engineering cartilage stent. The tissue engineering cartilage stent and the method have the advantages that the tissue engineering cartilage stent is excellent in biocompatibility, low in toxicity and easy to degrade, and degradation products are nontoxic; the tissue engineering cartilage stent is compact in appearance, and various performance parameters are adaptive to repairing cartilage tissues of human bodies; the constructed tissue engineering cartilage stent is made of wide material sources and easy to shape, and can be used for repairing wide-area wound, and the like.

The invention discloses a method for constructing tissue engineering cartilage. The method includes the steps of firstly, adding cartilage cell suspension to a Gelatin / PCL nanofiber electricity texture membrane; secondly, adding a Gelatin / PCL nanofiber electricity texture membrane to the cartilage cell suspension generated in the first step; thirdly, repeatedly executing the first step and the second step to obtain a composite; fourthly, culturing the composite in vitro to obtain the tissue engineering cartilage.

The invention discloses a joint-moving simulation test system with a biological culture function, which comprises a joint simulator and a biological culture circulating system, wherein the biological culture circulating system comprises a culture cavity, a dislocated sterilized glass fermentation tank and a liquid storage tank which are sequentially communicated, the culture cavity is composed of an upper cavity and a lower cavity which are communicated together, the upper cavity is communicated with the dislocated sterilized glass fermentation tank, and the lower cavity is connected with an inlet of the liquid storage tank; the upper cavity is connected with a horizontal axis of the joint simulator, and the lower cavity is connected with a longitudinal axis of the joint simulator. According to the joint-moving simulation test system disclosed by the invention, the joint simulator can realize the moving control on the culture cavity, and the dislocated sterilized glass fermentation tank can realize the control on the oxygen, carbon dioxide, pH value and temperature of culture liquid in the circulating system. The joint-moving simulation test system disclosed by the invention not only can carry out in-vitro culture on tissue engineered cartilages, but also can efficiently carry out an in-vitro friction and wear performance evaluation test on active joint cartilages.

The present invention provides an allogenic tissue engineered cartilage graft, it includes: (a) pharmaceutically acceptable bio-degradable material; and (b). cartilage cell, the described cartilage cell is one externally-cultured form primary culture to sixth generation. The invention can be used for effectively curing chondropathy. The invention also provides its preparation and application.