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Preparation method of nano-fiber scaffold for promoting cartilage regeneration

A nanofiber and cartilage technology, applied in tissue regeneration, fiber treatment, yarn, etc., can solve the problems of easy inactivation of growth factors, and achieve the effects of prolonged release time, good biocompatibility, and good microenvironment

Inactive Publication Date: 2018-03-16
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nell-1 is a new type of cartilage growth factor that can promote the function of chondrocytes, but this growth factor is easily inactivated during the process of incorporation into tissue engineering cartilage scaffolds, so it is urgent to find a new preparation method for nanofibrous scaffolds , to protect the biological activity of growth factors and prolong the release time of growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of Nell-1 loaded chitosan nanoparticles solution

[0030] a. dissolving chitosan (CS) in mass percent concentration is 0.5% aqueous acetic acid solution to prepare CS solution, the mass percent concentration of CS in CS solution is 30% (W / V), the weight average of chitosan The molecular weight is 80,000.

[0031] b. Dissolving TPP and CHS in deionized water to prepare a TPP solution and a CHS solution, the mass percent concentrations of the TPP solution and the CHS solution are both 3%.

[0032] c. Mix the CS solution, the TPP solution and the CHS solution at a ratio of 4:1:1 to prepare a chitosan nanoparticle (CSN) solution.

[0033] d. Dissolving Nell-1 in an equal volume of TPP and CHS mixture, and then adding the CSN solution prepared in c to prepare a Nell-1-loaded chitosan nanoparticle (Nell-1 / CSN) solution. The mass percent concentration of Nell-1 in the Nell-1 / CSN solution was 5%.

[0034] (2) Preparation of collagen / poly(L-lactic acid)-copoly...

Embodiment 2

[0041] (1) Preparation of Nell-1 loaded chitosan nanoparticles solution

[0042] a. dissolving chitosan (CS) in mass percent concentration is 0.1% aqueous acetic acid solution to prepare CS solution, the mass percent concentration of CS in CS solution is 10% (W / V), the weight average of chitosan The molecular weight is 50,000.

[0043] b. Dissolving TPP and CHS in deionized water to prepare a TPP solution and a CHS solution, the mass percentage concentrations of the TPP solution and the CHS solution are both 0.1%.

[0044] c. Mix the CS solution, the TPP solution and the CHS solution at a ratio of 2:1:1 to prepare a chitosan nanoparticle (CSN) solution.

[0045]d. Dissolving Nell-1 in an equal volume of TPP and CHS mixture, and then adding the CSN solution prepared in c to prepare a Nell-1-loaded chitosan nanoparticle (Nell-1 / CSN) solution. The mass percent concentration of Nell-1 in the Nell-1 / CSN solution is 1%.

[0046] (2) Preparation of collagen / poly(L-lactic acid)-cop...

Embodiment 3

[0053] (1) Preparation of Nell-1 loaded chitosan nanoparticles solution

[0054] a. dissolving chitosan (CS) in mass percent concentration is 1% aqueous acetic acid solution to prepare CS solution, the mass percent concentration of CS in CS solution is 40% (W / V), the weight average of chitosan The molecular weight is 100,000.

[0055] b. Dissolving TPP and CHS in deionized water to prepare a TPP solution and a CHS solution, the mass percent concentrations of the TPP solution and the CHS solution are both 5%.

[0056] c. Mix the CS solution, the TPP solution and the CHS solution at a ratio of 6:1:1 to prepare a chitosan nanoparticle (CSN) solution.

[0057] d. Dissolving Nell-1 in an equal volume of TPP and CHS mixture, and then adding the CSN solution prepared in c to prepare a Nell-1-loaded chitosan nanoparticle (Nell-1 / CSN) solution. The mass percent concentration of Nell-1 in the Nell-1 / CSN solution is 10%.

[0058] (2) Preparation of collagen / poly(L-lactic acid)-copoly(...

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Abstract

The invention belongs to the field of tissue engineering, and discloses a preparation method of a nano-fiber scaffold for promoting cartilage regeneration to solve the problem that Nell-1 serving as anovel cartilage-derived growth factor can promote cartilage bone cell functions, but is easily inactivated in the process of being integrated into a tissue engineering cartilage scaffold. The preparation method includes the steps: firstly, preparing Nell-1 supported chitosan nano-particle solution and collagen / poly (L-lactic acid)-copolymer (epsilon-caprolactone) mixed solution; secondly, preparing a PLLA-CL / collagen nano-fiber mesh by coaxial electro-spinning and dynamic liquid collection technology; finally, preparing the collagen / HA mixed nano-fiber scaffold by collagen and hyaluronic acid(HA). The operation method is simple, the prepared nano-fiber scaffold is good in biological compatibility, biological activity of the growth factor can be protected, the release time of the growth factor is prolonged, and cartilage repair and regeneration can be promoted.

Description

technical field [0001] The invention relates to a preparation method of a nanofibrous scaffold for promoting cartilage regeneration, which belongs to the field of tissue engineering. Background technique [0002] Damage to articular cartilage, caused by conditions such as trauma and osteoarthritis, is a major cause of pain and disease in the elderly. The avascularity and porosity of the cartilage structure limits its ability to self-renew. Traditional surgeries include bone marrow stimulation, cartilage-bone mosaic and artificial joint replacement, etc. However, all these surgeries can only relieve symptoms in the early stage, and the long-term effects are still controversial and unsatisfactory. [0003] The current rapidly developing cartilage tissue engineering technology provides a promising approach to solve the intractable cartilage regeneration problem. However, cartilage structures constructed in vitro are still not suitable for widespread clinical use due to their ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/24A61L27/34A61L27/50A61L27/54D01D5/00D02G3/02
CPCA61L27/18A61L27/20A61L27/24A61L27/34A61L27/50A61L27/54A61L2300/414A61L2430/06D01D5/0015D02G3/02C08L5/08C08L67/04C08L89/00
Inventor 赵亮亮许杉杉孟庆怡
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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