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Tissue engineered cartilage based on bone marrow mesenchymal stem cell

A bone marrow mesenchymal and tissue engineering technology, applied in the direction of bone/connective tissue cells, animal cells, tissue culture, etc., can solve the problems of insufficient mechanical strength, accumulation, and rapid degradation, and achieve high mechanical strength and less trauma. , the effect of convenient source of cells

Inactive Publication Date: 2005-01-19
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Application Information

AI Technical Summary

Problems solved by technology

Scaffold materials for cartilage tissue engineering need to have good biocompatibility, controllable degradability and sufficient mechanical characteristics to promote and support the regeneration of new cartilage. Today's cartilage tissue engineering scaffold materials, such as collagen, chitosan , polymers, etc. have shortcomings such as insufficient mechanical strength, accumulation of metabolic acid products, and excessive degradation speed, while inorganic biomaterials, such as porous bioceramics, that is, β-tricalcium phosphate bioceramics (β-TCP) or α-TCP, because of Its good osteoinductive properties and mechanical strength are mostly used in the repair of bone tissue, but we recently found that β-TCP also has good cartilage inductive properties, whether it is composite chondrocytes or composite MSCs, it can well repair articular cartilage defects

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0012] Example 1 Construction of tissue engineered cartilage

[0013] 1) The method of in vitro isolation and culture of bone marrow mesenchymal stem cells: 6-month-old Merino sheep weighing 25Kg±, ketamine 0.01g / kg intramuscular injection anesthesia, shearing and disinfection of the posterior iliac crest, and puncture with an 18-gauge bone puncture needle And aspirate bone marrow fluid into a syringe containing 0.1ml (3000u / ml) heparin, first centrifuge (1000rpm×5min), aspirate the plasma, then add an equal volume of L-DMEM to mix, and then use a density of 1073g / l Percol separation solution 900g×25 minutes to separate nucleated cells. After washing and centrifugation, mix it with L-DMEM containing 10% of its own plasma, and pipette evenly to 1×10 5 / cm 2 Inoculate it in a culture flask at a density that is 48 hours later, change the medium to remove non-adherent cells, and then change to a culture medium containing 10% fetal bovine serum after the appearance of cell colonies.

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Embodiment 2

[0015] Example 2 Application of tissue engineered cartilage in the repair of sheep articular cartilage defects

[0016] 1) Implantation in the body: Open and expose the medial femoral condyle of the knee joint after anesthesia and disinfection. Use a trephine with an outer diameter of 8mm to drill holes in the weight-bearing area to a depth of 4mm to artificially create a large section of full-thickness articular cartilage defects and implant them separately The MSC-β-TCP complex was used as the experimental group, and the simple β-TCP repair and defect vacant were used as the control group. The implant is tightly combined with the defect, the surface and the adjacent surface are continuously leveled, the wound is closed by suture, and the weight is gradually moved after immobilization for 2 weeks.

[0017] 2) Results: After 12 weeks of sampling, it was found that obvious translucent new cartilage tissue had been formed in the experimental tissue defect area. The histological resu...

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Abstract

The invention belongs to tissue engineering field. It makes self body mesenchymal stem cell as seed cell and porous biological ceramics as bracket material. Adding proliferating agent and differentiating agent when cultivating the complex in vitro, transplanting and restoring articular cartilage injury after cultivating in vitro for a period of time The invention is characterized in that it constructs tissue engineering cartilage by employing marrow mesenchymal stem cell and porous biological ceramics for restoring articular cartilage injury. It has an preferably clinical practice prospect.

Description

Technical field [0001] The invention relates to the field of tissue engineering for manufacturing human organs and tissues, in particular to a tissue engineered cartilage and its application in repairing articular cartilage defects. Background technique [0002] Cartilage tissue is a highly differentiated terminal tissue with no blood vessels, lymphatic vessels, and no innervation. It is difficult to repair itself after injury. If effective treatment is not available after injury, it will often cause articular cartilage degeneration and eventually lead to Osteoarthritis, with symptoms such as pain, joint stiffness, and dysfunction. The current clinical treatment methods such as weight-bearing, infrared hyperthermia, electromagnetic stimulation, and intra-articular injection of transforming growth factor (TGF-β) and other biologically active factors are difficult to effectively repair cartilage damage, and can only temporarily relieve symptoms and cannot be effective. Promote cart...

Claims

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Application Information

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IPC IPC(8): A61L27/00A61L27/10C12N5/0775
Inventor 王常勇张玉富郭希民段翠密赵强
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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