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Injectable cartilage repairing hydrogel and preparation method thereof

A cartilage repair and hydrogel technology, applied in medical science, prosthesis, etc., can solve the problem of low mechanical strength of gelatin, and achieve the effect of good injectability and good biocompatibility

Inactive Publication Date: 2019-05-03
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gelatin itself has low mechanical strength and is rarely used alone as a cartilage repair material

Method used

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  • Injectable cartilage repairing hydrogel and preparation method thereof
  • Injectable cartilage repairing hydrogel and preparation method thereof
  • Injectable cartilage repairing hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An injectable hydrogel for cartilage repair, comprising the following components in mass percentage: 1% modified hyaluronic acid, 10% modified gelatin, 1% platelet-rich plasma, 1% ultraviolet photoinitiator, and the balance is water.

[0033] The preparation method of the present embodiment hydrogel material comprises the following steps:

[0034] (1) Preparation of modified hyaluronic acid

[0035] Weigh 5g of hyaluronic acid, add 100mL of ionized water, stir until completely dissolved at room temperature, add 25g of methacrylic anhydride, adjust the pH to 8 with 1mol / L NaOH, stir and react in an ice-water bath overnight, and then load 12-14KDa In the dialysis bag, dialyze with pure water for 3 days, then freeze-dry to obtain modified hyaluronic acid, and store it at room temperature for later use;

[0036] (2) Preparation of modified gelatin

[0037] Weigh 20g of gelatin, add 100mL of deionized water, stir in a water bath at 50°C until completely dissolved, add 12g ...

Embodiment 2

[0045] An injectable hydrogel for cartilage repair, comprising the following components in mass percentage: 1% modified hyaluronic acid, 10% modified gelatin, 1% platelet-rich plasma, 2% ultraviolet photoinitiator, and the balance is water.

[0046] The preparation method of the hydrogel material in this example is the same as that in Example 1, wherein, in step (4), the modified hyaluronic acid and the modified gelatin are prepared into 2% and 20% solutions respectively, and then mixed and stirred evenly in equal volumes; Then 1% of platelet-rich plasma and 2% of ultraviolet photoinitiator were added respectively.

[0047] Within two weeks, the sustained release of VEGF in the gel material of this embodiment can reach about 90%; the sustained release of TGF-β1 within two weeks is close to 85%, indicating that the hydrogel material loaded with platelet-rich plasma in this embodiment can achieve both. slow release of growth factors.

Embodiment 3

[0049] An injectable hydrogel for cartilage repair, comprising the following components in mass percentage: 1% modified hyaluronic acid, 7.5% modified gelatin, 1% platelet-rich plasma, 1% ultraviolet photoinitiator, and the balance is water.

[0050] The preparation method of the hydrogel material in this example is the same as that in Example 1, wherein, in step (4), the modified hyaluronic acid and the modified gelatin are respectively configured into 2% and 15% solutions, and then mixed and stirred evenly in equal volumes; Then 1% platelet-rich plasma and 1% ultraviolet photoinitiator were added respectively.

[0051] Within two weeks, the sustained release of VEGF in the gel material of this embodiment can reach about 90%; the sustained release of TGF-β1 within two weeks is close to 85%, indicating that the hydrogel material loaded with platelet-rich plasma in this embodiment can achieve both. slow release of growth factors.

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Abstract

The invention relates to an injectable cartilage repairing hydrogel and a preparation method thereof, which belong to the technical field of biological materials. The injectable cartilage repairing hydrogel of the present invention comprises modified hyaluronic acid, modified gelatin, a platelet rich plasma, and an ultraviolet photoinitiator. The materials of the cartilage repairing hydrogel of the invention have good biocompatibility and injectability, the platelet-rich plasma can achieve a variety of growth factors, such as vascular endothelial growth factors, transforming growth factors, and the like, can be used in tissue engineering cartilage repairing.

Description

technical field [0001] The invention relates to an injectable cartilage repair hydrogel and a preparation method thereof, belonging to the technical field of biomaterials. Background technique [0002] Cartilage tissue is composed of chondrocytes and extracellular matrix. Unlike bone, which has great regenerative potential, cartilage has limited repair ability due to lack of blood supply support. Chondrocytes maintain their native phenotype in a three-dimensional scaffold resembling their natural environment and produce the building blocks of the extracellular matrix, which in turn promotes cartilage tissue regeneration. Hydrogel can maintain a fluid state under certain conditions, and form a body-shaped material with a certain shape and strength under external stimuli. This intelligence can be used to prepare injection-type scaffolds, and play its role in repairing complex-shaped defects and minimally invasive treatments. advantages. Therefore, research and development of...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/54A61L27/52A61L27/50
Inventor 蓝咏刘玉李照文毛宇冯龙宝
Owner BEOGENE BIOTECH GUANGZHOU
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