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Method for constructing tissue engineering cartilage

A tissue engineering and construction method technology, applied in bone implants, medical science, prostheses, etc., can solve the problems of incomplete decellularization, complicated preparation process, and difficulty in re-growing cells.

Inactive Publication Date: 2014-03-12
SHANGHAI TISSUE ENG LIFE SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The decellularized cartilage matrix has the advantages of natural composition and similar mechanical strength to normal cartilage tissue. However, because cartilage is a very dense connective tissue, there are two obstacles: incomplete decellularization and difficulty in the growth of new cells.
In order to solve the above problems, people have tried many methods, but the results are not satisfactory.
However, large pieces of cartilage have problems such as incomplete decellularization and difficulty in re-growth of cells. At the same time, the preparation process of decellularized cartilage membranes is complicated and difficult to standardize.

Method used

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  • Method for constructing tissue engineering cartilage
  • Method for constructing tissue engineering cartilage
  • Method for constructing tissue engineering cartilage

Examples

Experimental program
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Effect test

Embodiment

[0042] Materials and methods

[0043] 1. Preparation of Gelatin / PCL nanofiber electrospun membrane

[0044] Gelatin:PCL ratios of Gelatin / PCL nanofiber electrospun membrane materials used in this example include 30:70, 50:50, and 70:30, which were purchased from the Institute of Biology, Donghua University. The material was processed into a circular diaphragm with a diameter of 9 mm by corneal trephine, and it was processed in a vacuum freeze dryer for 24 hours before use (aseptic).

[0045] 2. Culture of auricle chondrocytes

[0046] The auricular cartilage tissue of newborn pigs was taken, cut into 1×1×1 mm size, digested overnight with 0.1% collagenase, filtered through a filter with a pore size of 75 μm, added high-sugar DMEM (Hyclone, USA) containing 10% fetal bovine serum ( FBS) (Hyclone, USA) culture medium, placed in 5% CO 2 Cultured in a 37°C incubator, cultivated to 80%-90% confluence and then passaged.

[0047] 3. Sandwich method to build cartilage

[0048] The...

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Abstract

The invention discloses a method for constructing tissue engineering cartilage. The method includes the steps of firstly, adding cartilage cell suspension to a Gelatin / PCL nanofiber electricity texture membrane; secondly, adding a Gelatin / PCL nanofiber electricity texture membrane to the cartilage cell suspension generated in the first step; thirdly, repeatedly executing the first step and the second step to obtain a composite; fourthly, culturing the composite in vitro to obtain the tissue engineering cartilage.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a method of constructing tissue engineering cartilage by using a degradable artificial synthetic membrane material. Background technique [0002] Cartilage is an avascular tissue. Because of its low self-repair ability, cartilage damage or deformity has always been a major problem in clinical treatment. Tissue engineering technology provides a new treatment method for cartilage defect repair. Degradable bio-scaffold materials are one of the basic elements of tissue engineering. At present, the commonly used scaffold materials for constructing cartilage include polylactic acid (polyglycolic acid, PGA), type I collagen, and cartilage decellularized matrix. Compatibility, non-immunogenicity and other advantages have become a research hotspot in recent years. The acellular matrix of cartilage has the advantages of natural composition and similar mechanical strength to normal carti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28A61L27/38A61L27/24A61L27/18
CPCA61F2/30756A61F2002/30001
Inventor 张文杰薛继鑫周广东刘伟曹谊林
Owner SHANGHAI TISSUE ENG LIFE SCI
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