133 results about "Pseudomonas cichorii" patented technology

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu/mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The invention discloses a pseudomonas sp. as well as a cultivation method and application thereof. The pseudomonas sp. is preserved in General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation No. is CGMCC No. 13433. The cultivation method comprises the following steps: inoculating the pseudomonas sp. preserved on a solid medium in an enrichment medium and performing shake cultivation at the constant temperature of 30 DEG C at 200rpm for 12h. The application of the pseudomonas sp. comprises application in biological denitrification of sewage and application in degradation of phenol. The pseudomonas sp. CGMCC No. 13433 has a very good application prospect in treatment of water with overproof ammonia nitrogen and water polluted by phenol in industrial and agricultural water and underground water.

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

The invention discloses a rhodopseudomonas palustris CCTCC M 2012224, and application of the rhodopseudomonas palustris CCTCC M 2012224 in prevention and control of rice false smut or improvement of catalase activity in the rice body, and further discloses an antagonistic fungicide obtained by activation, seed culture and production culture of the strains. Fermentation supernate obtained through fermentation and centrifugation of the antagonistic fungicide can be used as an antagonistic pesticide; thallus crushing liquid obtained by fermentation culture and crushing of the antagonistic fungicide can also be used as the antagonistic pesticide. The antagonistic fungicide and the antagonistic pesticides obtained both can be used for preventing and controlling the rice false smut and improving the catalase activity in the rice body. The rhodopseudomonas palustris CCTCC M 2012224, the antagonistic fungicide and the antagonistic pesticides have the advantages of low production cost, nontoxicity, environmental friendliness and the like, is convenient to use, and can be used for preventing and controlling the rice false smut and improving the disease resistance of rice.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention relates to Pseudomonas sp. LD23, and the strain preservation number is CGMCC No,11679. The invention further relates to application of the Pseudomonas sp. LD23 in petroleum degradation and preparation of sodium alginate-activated carbon immobilized microspheres containing the Pseudomonas sp. LD23. The Pseudomonas sp. LD23 is a strain separated and screened from soil which is serious in petroleum pollution, can keep the high petroleum degradation rate under the low temperature condition and lays a foundation for petroleum-polluted soil treatment in future; meanwhile, the biological carbon immobilization conditions are optimized, the degrading effect of the immobilized petroleum degrading bacterium to raw petroleum is initially inspected, and a scientific basis is provided for remedying petroleum oil through the biological carbon immobilized microspheres.

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses an acid-resistant pseudomonas koreensis strain CLP-7 which is capable of preventing diseases and promoting growth, and can be used for biocontrol. The acid-resistant pseudomonas koreensis strain CLP-7 is preserved at China General Microbiological Culture Collection Center, on 27th, October, 2016, and the preservation number is CGMCC No.13204. The antibacterial spectrum of the acid-resistant pseudomonas koreensis strain CLP-7 is relatively large; the acid-resistant pseudomonas koreensis strain CLP-7 is capable of inhibiting growth of Phytophthora parasitica var. nicotianae, Ralstonia solanacearum, and Alternaria alternata Keissler; the antagonistic activity under acidic conditions is high, the acid-resistant pseudomonas koreensis strain CLP-7 is capable of producing siderophore, possesses protease and glucanase activity, and potassium releasing capacity. The results of biocontrol pot experiment show that the acid-resistant pseudomonas koreensis strain CLP-7 is capable of preventing under acidic soil conditions, and promoting growth and chlorophyll synthesis of tobacco seedlings in acidic soil, so that the acid-resistant pseudomonas koreensis strain CLP-7 and microorganism bacterium agents of the acid-resistant pseudomonas koreensis strain CLP-7 can be used for preventing tobacco fungi and bacterial root and stem diseases under continuous cropping or acidic soil conditions effectively, and is high in application value.

Recombinant bacterial strains comprising heterologous nif genes in its genome, and capable of fixing nitrogen. The strain may be, for example, a recombinant Pseudomonas fluorescens strain comprising heterologous nif genes in its genome. An inoculum and a method for increasing plant productivity are further described.

The invention relates to the technical field of pseudomonas mosselii, in particular to pseudomonas mosselii. The pseudomonas mosselii is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 12222. The pseudomonas mosselii has an antibacterial effect on pathogenic bacteria of rice blast, rice bakanae disease, rice sheath blight disease, rice seedling blight and rice false smut.

The invention discloses endophytic azotobacter of wheat producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application thereof. The endophytic azotobacter of wheat is pseudomonas sp. GDXM114, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.5040. The pseudomonas sp. GDXM114 with the collection number of CGMCC No.5040 has the advantages of higher nitrogenase activity, ACC deaminase activity and wide application prospect in producing nitrogenase microorganism inoculants and microbial organic fertilizers.

The invention relates to a repairing agent for microbes in water bodies of excessive culture zones, which comprises the following bacteria: Bacillus, Pseudomonas, Bacillus pumilus and Acinetobacter; the collection institution is the China General Microbiological Culture Collection Center (CGMCC); the collection numbers are respectively CGMCC No: 3737, 3739, 3752 and 3712; and the mass ratio of the Bacillus to the Pseudomonas to the Bacillus pumilus to the Acinetobacter is 1.4-1.7:1.0-1.4:0.9-1.1:1.2-1.5. The repairing agent of the invention is a microbial ecological agent which can be used for repairing excessive culture zones of water environment, is harmless to culture organisms, can inhibit propagation and growth of harmful microbes in water bodies, purifies the water quality and improves the quality and the performance of aquatic products.

The invention belongs to the application field of microorganisms, in particular to a rapeseed pseudomonas with plant growth promoting effect. The specific technical scheme is as follows: a strain of rapeseed pseudomonas is deposited in the General Microbial Center of China Microbial Culture Collecting Management Committee on July 30, 2018, and the deposit number is CGMCC No. 16170. The rapeseed pseudomonas has excellent salt tolerance, can maintain strong vitality and excellent ability of secreting plant hormones at high salt concentration, and can secrete chitinase and degrade chitin, thus having certain effect on controlling plant fungal diseases. The results show that the strain of Pseudomonas campestris has good salt tolerance, and can be used for controlling plant fungal diseases. Atthe same time, the bacterium also has good potassium and phosphorus solubilization effect, and can be practically used in saline-alkali soil improvement.

The invention provides a Korean pseudomonas and application thereof. The Korean pseudomonas is identified as Korean pseudomonas CJ-361, and the preservation number is CGMCC NO.12122. The Korean pseudomonas CJ-361 is obtained through separation from radix pseudostellariae rhizosphere soil by adopting a pseudomonas selectivity culture medium, an antagonistic experiment is performed on fusarium oxysporum and fusarium moniliforme separated to the radix pseudostellariae root lesion portion through an agar plate opposite culture method, and it is found that the Korean pseudomonas has the strong antagonism on the fusarium oxysporum and the fusarium moniliforme. Meanwhile, the invention provides the optimum culture medium for antagonistic bacterium growth, the optimum formula is 1/4LB+1/20MS (containing no ferric salt)+0.1 wt% brown sugar, and the fermented liquor has the obvious antagonistic effect. The antagonistic strain and the microbial agent thereof can effectively prevent and treat radix pseudostellariae root rot diseases, and the environment-friendly, ecological and safe biological prevention and control potential strain is provided for overcoming or relieving replantation diseases of radix pseudostellariae.

The invention provides a compound microbial bacterium for an aerobic fermentation stage. The compound microbial bacterium for an aerobic fermentation stage is prepared by culturing, fermenting, drying and mixing Bacillus licheniformis, Bacillus subtilis, saccharomycete, 5406 actinomycete, Trichoderma viride, Aspergillus oryzae and pseudomonad respectively, wherein the compound microbial bacterium comprises the following components in parts by weight: 0.7-1.2 parts of Bacillus licheniformis, 0.7-0.8 part of Bacillus subtilis, 1.1-1.2 parts of saccharomycete, 1.5-2.5 parts of 5406 actinomycete, 1-2 parts of Trichoderma viride, 1-2 parts of Aspergillus oryzae and 0.5-1 part of pseudomonad. The compound microbial bacterium for an aerobic fermentation stage has phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing capabilities, and can quickly decompose organic substances, so that materials are thoroughly decomposed, and oxidation-resistant substances are generated through metabolism, thus generating a complex and stable ecological system; and the heating speed is high in the whole fermentation process, the temperature can be up to 50 DEG C or above within 1-2 days, and undesirable odor starts to disappear within 24 hours.

The invention relates to a method for producing liquid organic bacterial manure by utilizing the waste liquor of restaurants and kitchens. The method comprises the following steps of: selecting bacillus megaterium, candida lipolytica and pseudomonas; preparing a pseudomonas slope culture medium, a bacillus megaterium slope culture medium and a candida tropicalis slope culture medium; conveying the pseudomonas, the candida lipolytica and the bacillus megaterium into the fresh slope culture mediums respectively, and culturing at the temperature of 30 DEG C for 24 hours and activating; putting the activated strains into a culture medium in which agar is removed respectively, and culturing at the temperature of 30 DEG C for 18 hours at the rotation speed of 180 revolutions/minute of a shaker to obtain activated bacterial liquid, wherein the inoculation amount is 2 percent; inoculating the activated bacterial liquid of the pseudomonas, the bacillus megaterium and the candida lipolytica into the rubbish waste liquor of restaurants and kitchens in a ratio of 1:1:1, wherein the inoculation amount is 2 percent; adding 60 percent excrement, which serves as auxiliary materials, of livestock and poultry to prepare culture expanding bacterial liquid; and fermenting the compounded bacterial liquid at the temperature of 30 DEG C for 72 hours to obtain the liquid organic bacterial manure.

The invention provides a pseudomonas sp. strain 2-29, which belongs to the class of pseudomonas sp. and was collected with a collection number of CGMCC No.3331 in China General Microbiological Culture Collection Center (abbreviated as CGMCC) of Institute of Microbiology Chinese Academy of Sciences located on Datun road in Chaoyang district of Beijing, on October 2009. The invention also provides the use of the pseudomonas sp. strain 2-29 in the prevention and control of tomato ralstonia solanacearum. For solving the problem that an effective chemical for treating tomato ralstonia solanacearum lacks at present and the problem that pseudomonas sp. is susceptible to generating medicament resistance to chemical pesticide, a strain having an antagonistic effect on tomato ralstonia solanacearum is screen through screening tomato ralstonia solanacearum antagonists and the test of the control effect of the tomato ralstonia solanacearum antagonists; on this basis, field tests can be further performed for developing a mature microbial fertilizer; and thus, the pseudomonas sp. strain 2-29 has a great significance for reducing the use of a large amount of fertilizer and pesticide and reducing agricultural pollution sources.

The invention discloses a pseudomonas strain and application thereof. The strain is pseudomonas protegens IAS03, and is preserved in the China center for type culture collection on November 27th, 2015, and the preservation number is CCTCC NO:M 2015711. The strain is screened from pond bottom sludge and then a backwater body is used, so that ecological safety risks do not exist, and biological safety regulations are conformed. The strain has the specific antimicrobial function on aeromonas hydrophila and aeromonas sobria, has no inhibition effect on other bacteria and has no pathogenicity on fishes. Only one strain can be used for inhibiting aeromonas hydrophila and/or aeromonas sobria. Compared with traditional antibiotic and sterilizing agent prevention measures, the strain has the advantages of being safe, environmentally friendly and efficient. The strain can be developed into a microecological preparation to control aeromonas sobria and aeromonas hydrophila in the breeding environment and prevent hemorrhagic septicemia caused when the fishes are infected with aeromonas sobria and aeromonas hydrophila.

The invention discloses a biological control pseudomonas monteilii strain against tobacco mosaic virus (TMV). The code name of the strain is 4A1, the strain is collected in China General Microbiological Culture Collection Center (CGMCC) on September 7, 2011, and the collection number of the strain is CGMCC No. 5229. The strain can inhibit the infection of the TMV, and a biological control experiment shows that the strain can control the TMV. Simultaneously, the invention further provides a method for separating active substances from fermentation supernatant fluid obtained from the strain, and the active substances have a very high application value in the aspect of controlling the TMV.