Pseudomonas mosselii and application thereof
A technology of pseudomonas and engineering bacteria, applied in the field of pseudomonas, can solve problems such as environmental and ecological pollution and harm
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[0032] 1. Collection of soil samples
[0033] Soil samples were randomly collected from Taoyuan Mountain in Lijiang, Yunnan. Remove the surface soil, collect about 300g of soil samples at a depth of 5-20cm, and bring them back to the laboratory after subpackaging and marking.
[0034] 2. Isolation of strains
[0035] Separate by soil dilution method, weigh 10g of soil and pour it into a 90ml sterile water triangular flask equipped with glass beads, this is 10 -1 After shaking for 10 minutes, draw 1ml into a new triangular flask filled with 90ml of sterile water, shake and mix well, this is 10 -2 The diluent, according to this method continue to dilute to 10 -4 , 10 -5 , 10 -6 diluent. draw 10 -4 、10 -5 and 10 -6 Add 100 μl of the diluent to the KB solid medium plate, repeat each gradient 3 times, spread evenly, place it upside down in a 28°C incubator and cultivate it for 24 hours, then streak and purify typical colonies on the KB medium plate for 2-3 times A single ...
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