Application of psoralen as agricultural disinfectant or pesticide
A technology of psoralen and insecticide, applied in the agricultural field, can solve the problem of no psoralen fungicide or insecticide, and achieve the effects of favorable environmental protection, broad antibacterial spectrum and remarkable effect
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Embodiment 1
[0013] Embodiment 1, the prevention and treatment of psoralen to agricultural fungus disease
[0014] The tested strains Fusarium graminearum (Fusarium.graminearm) were isolated from wheat head blight; F.oxysporum (F.oxysporum) were isolated from cucumber wilt diseased plants; solani) was isolated from rice sheath blight leaves; Rhizoctonia solani was isolated from wheat sheath blight leaves; Sclerotioia selerotiorum was isolated from Sclerotinia sclerotiorum strains. The test drug psoralen (purchased from China National Institute for the Control of Pharmaceutical and Biological Products) was dissolved in acetone to make a 1000 μg / mL stock solution, and stored at 4°C for later use. PDA medium: cut 200g of peeled potatoes into pieces, boil for 10min, take the juice, add 20g of agar, heat until melted, then add 20g of glucose, and dilute to 1000mL with water.
[0015] Adopt the mycelia growth rate determination method to the inhibitory measurement of fungal mycelial growth, get...
Embodiment 2
[0019] Example 2 Psoralen is used as an active ingredient in the prevention and control of agricultural pests
[0020] The source of the tested insects was the 3rd to 6th instar larvae collected from the field population, raised indoors with artificial feed until pupation and laying eggs, and the 2nd to 3rd instar larvae with basically the same size as the F1 and F2 generations in the room were selected for toxicity testing (among them, vegetable caterpillars were collected from the field). Accurately weigh psoralen (purchased from China Institute for the Control of Pharmaceutical and Biological Products), dissolve it in acetone, and make a concentration of 2.000mg / ml mother solution for testing, then dilute it into 6 pieces with 1% TritonX-100 water emulsion. Different concentrations were used as the test liquid for standby use. Fresh and clean cabbage leaves (wherein, armyworms use wheat leaves and tea geometrids as fresh tea leaves) are made into leaf discs with a diameter...
Embodiment 3
[0025] Psoralen, lignin, diatomaceous earth and other conventional auxiliary materials were weighed to prepare a wettable powder with an active ingredient concentration of 2% according to a conventional process.
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