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Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application thereof

A technology of Pseudomonas chlorophyll, antibacterial activity, applied in application, bacteria, bactericide, etc., to achieve the effect of lasting antibacterial effect

Active Publication Date: 2017-08-29
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, Pseudomonas chloropinus is mainly used for the effective control of diseases caused by pathogenic fungi and bacteria in various vegetable crops, while Pseudomonas chloropinus against fungal diseases of fruit trees and field crops has not been reported.

Method used

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  • Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application thereof
  • Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application thereof
  • Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Obtaining of Pseudomonas chloropinus YTBTa14 Strain

[0038] First, collect healthy dandelion plants from the field, wash the surface with tap water and dry them, then take the roots, stems, and leaves for surface disinfection, add 10 ml of pure water to grind, let stand for 10 minutes, take 80ul supernatant, dilute Spread on NA solid medium and culture in a 28°C incubator for 3-7 days. Pick a single colony according to the characteristics of the colony shape, color, etc., and store it on the slant medium after purification.

[0039] Thirdly, Pseudomonas chloropinus YTBTa14 strains that antagonize various diseases of fruit trees and field crops were screened by the plate confrontation culture method. Cultivate apple ringworm, grape anthracnose, grape white rot, cherry phytophthora, and wheat sheath blight on a special medium, and after the pathogens are overgrown on the plate, use a puncher to punch out the bacteria on the edge of the plate. Then put it in the center ...

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Abstract

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

Description

technical field [0001] The invention relates to Pseudomonas chloropinus with broad-spectrum antibacterial activity and the application of the strain in the prevention and control of fruit tree and field crop diseases, belonging to the field of biotechnology. Background technique [0002] Wheat and fruit trees are important economic crops in the world. They are harmed by many diseases all the year round, and the annual economic losses caused by diseases are difficult to estimate. At present, pesticides are one of the most effective means of preventing and controlling pests and diseases, but various problems caused by the widespread use of chemical pesticides have also followed, such as pesticide residues, environmental pollution, and the emergence of resistance to pests and diseases, which are not conducive to the development of agriculture. sustainable development. Therefore, the prohibition and restriction of the use of a large number of highly toxic and high residue pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 李宝燕王英姿王培松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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