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Aspergillus niger with dual-function of phosphorus-dissolving and bio-control as well as microorganism preparation and application

A microbial preparation, dual-function technology, applied in the field of Aspergillus niger and microbial preparations, can solve problems such as uncommon, and achieve the effect of strong dephosphorization ability

Pending Publication Date: 2017-05-31
武汉合缘绿色生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening of phosphate-solubilizing bacteria mainly comes from the rhizosphere soil of plants, but it is not common in terms of current application

Method used

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  • Aspergillus niger with dual-function of phosphorus-dissolving and bio-control as well as microorganism preparation and application
  • Aspergillus niger with dual-function of phosphorus-dissolving and bio-control as well as microorganism preparation and application
  • Aspergillus niger with dual-function of phosphorus-dissolving and bio-control as well as microorganism preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preliminary Screening of Phosphorus Solubilizing Microorganisms

[0025] Collect the soil with mild wheat head blight, weigh 10g, put it in a triangular flask filled with 100mL of sterile water, shake it at 28°C and 180rpm for 20min, and let it stand for 10min to obtain a soil suspension. Dilute the suspension to a suitable concentration and spread it on the primary screening medium plate of phosphate-solubilizing bacteria, culture it upside down at 28°C, observe every 2 days, pick out the colonies with phosphorus-dissolving circles, and re-inoculate on the phosphate-solubilizing bacteria by streaking method. Bacteria primary screening medium plate for purification, repeated 2 to 3 times, purified to a single colony. Inoculate the strains with phosphorus-dissolving effect after purification on the primary screening medium plate of phosphate-solubilizing bacteria, and culture them upside down at 28°C for 7 days, and measure the diameters of phosphorus-dissolvi...

Embodiment 2

[0029] Embodiment 2: shake flask re-screening of phosphorus-solubilizing microorganisms

[0030] Test strains: 5 strains of phosphate-solubilizing bacteria obtained from preliminary screening.

[0031] Prepare re-screening medium for phosphate-solubilizing bacteria, pack in 250mL Erlenmeyer flasks, 50mL per bottle, and sterilize for later use. After activating the 5 strains of phosphate-solubilizing bacteria obtained from the preliminary screening, use a slant to make a uniform 5mL bacterial suspension, inoculate 1mL bacterial suspension into each triangular flask, inoculate 3 triangular flasks, and make 3 non-inoculated controls at the same time, Place them on a shaker (130r / min) at 28°C for 7 days and then set them aside for later use. Add phosphorus-free activated carbon to the fermentation broth for decolorization and centrifugation for 15min (8000r / min), and keep the supernatant. Take 10mL of the supernatant into a 50mL volumetric flask, dilute to 30mL with water, add 2...

Embodiment 3

[0037]Embodiment 3: the identification of phosphate solubilizing bacteria HY30

[0038] Colony morphology: It grows fast on PDA medium. After 24 hours, colonies appear, and after 72 hours, spores are abundant; the colonies are black and velvety, with a slightly raised center, and the opposite side of the colonies is slightly yellow. Under the microscope, the flora is dark brown with well-developed hyphae. The conidia head is like a "chrysanthemum" with two layers of spore stalks. The conidia are spherical and black or dark brown. figure 1 and figure 2 These are the morphology of HY30 on the PDA plate and under the microscope.

[0039] Molecular biology identification of HY30 strain: The 18SrDNA of HY30 was detected by molecular biology method

[0040] Sequences were blasted against the GenBank database. Combining the results of colony morphology and 18SrDNA comparison, the applicant determined that the HY30 strain was Aspergillus niger. The strain was deposited in the Chi...

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Abstract

The invention provides aspergillus niger with dual-function of phosphorus-dissolving and bio-control. The aspergillus niger is preserved in the China Center for Type Culture Collection (CCTCC) on August 25, 2016; the preservation number is CCTCC NO: M2016427; the classification name is aspergillus niger HY30. The viable bacterium amount of the aspergillus niger in a microorganism preparation containing the strain is 10<8>CFU / g at least; the aspergillus niger has a phosphorus-dissolving function and also has the effect of preventing and treating soil-borne diseases.

Description

technical field [0001] The invention relates to an aspergillus niger and microbial preparation with dual functions of phosphorus solubilization and biocontrol and its application. Background technique [0002] Phosphorus is one of the important nutrient elements for organisms. Phosphorus must be involved in the photosynthesis of plants and the biochemical processes in the body. Increasing phosphate fertilizer is a "high input, low output" approach, which not only causes a lot of waste of phosphorus resources, seriously affects the sustainable development of the phosphate fertilizer industry, but also reduces economic benefits and aggravates environmental pollution. Most of the agricultural soils in the world are deficient in phosphorus (total phosphorus is not lacking, but available phosphorus is deficient), and about two-thirds of the farmland in my country is severely deficient in phosphorus. Phosphorus in soil usually exists in the form of phosphate, which can be divided...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C05F11/08C12R1/685
CPCA01N63/30C05F11/08C12N1/145C12R2001/685
Inventor 杨涛熊峰徐维烈徐辉张明军贺文杰胡定志
Owner 武汉合缘绿色生物股份有限公司
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