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Microbial fertilizer specially for beef-wood

A microbial fertilizer, casuarina technology, applied in the directions of organic fertilizers, fertilizer mixtures, applications, etc., can solve the problems affecting the symbiotic nitrogen fixation and self-fertilization ability of casuarina, low nodulation rate of casuarina, weak symbiotic ability, etc., and achieve rich extracellular secretions. , the spore survival time is long, the effect of strong resistance

Inactive Publication Date: 2010-10-20
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor nitrogen fixation efficiency and weak symbiosis ability of Frankia in the soil, the nodulation rate of casuarina under natural conditions is low and most of them are invalid root nodules, which seriously affects the symbiotic nitrogen fixation and self-fertilization ability of casuarina

Method used

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  • Microbial fertilizer specially for beef-wood
  • Microbial fertilizer specially for beef-wood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Mix 5g of peptone, 3g of beef extract, 5g of NaCl, and 15g of agar, make the volume to 1000mL with distilled water, adjust the pH to 7.0-7.2, sterilize at 121°C for 30 minutes at a pressure of 103kPa, and incubate at 28-30°C for 24 hours to obtain commonly used A culture medium for Bacillus megaterium phosphate solubilizers. Will KH 2 PO 4 0.95g, K 2 HPO 4 0.435g, MgSO 4 0.096g, CaCl 2 0.01g, NH 4 Cl0.0535g, sodium propionate 0.48g, FeSO 4 0.0056g and 0.0075g of NaEDTA were dissolved in 900mL of distilled water to obtain A solution. H 3 BO 3 2.86g, MnCl 2 1.81g, ZnSO 4 0.22g, CuSO 4 0.08g, Na 2 MoO 4 0.025g, CoCl 2 0.025g was dissolved in distilled water and the volume was adjusted to 1000mL to obtain B solution. VB 1 0.010g, niacin 0.050g, biotin 0.015g, folic acid 0.010g, calcium pantothenate 0.010g, riboflavin 0.010g, VB 6 0.050g was dissolved in distilled water and the volume was adjusted to 100mL to obtain C solution. Add 1mL of B sol...

Embodiment 2

[0021] Dissolve 0.1g (0.01%) of flavonoids, 0.01g (0.001%) of coumarin, 0.01g (0.001%) of sodium molybdate and 0.1g (0.01%) of cobalt sulfate in 1000mL tap water to obtain a stabilizing additive.

[0022] The Bacillus phosphate solubilis fermented liquid obtained in Example 1, the Frankia fgc6 strain fermented liquid and the stabilizing auxiliary agent are mixed in a volume ratio of 1: 2: 0.5 to obtain microbial fertilizers, and are directly sprayed onto seedlings by the method of spraying seedlings. The fertilizer can pour 500 to 800 seedlings. Spray the seedlings with clean water for half an hour after pouring the bacterial fertilizer. Measure the height of the seedlings, the number of root nodules, the dry weight of the aboveground part, the dry weight of the underground part and the total dry weight (experimental group), and without spraying any fertilizer (control group 1), only spraying Frankia fgc6 strain fermentation liquid (control group 2), only spraying Bacillus phos...

Embodiment 3

[0026] The microbial fertilizer obtained in Example 2 was mixed with the organic fertilizer at a mass ratio of 1:1, and the microbial fertilizer was adsorbed on the organic fertilizer to obtain experimental group 1. The microbial fertilizer obtained in Example 2 is mixed with zeolite powder in a mass ratio of 0.5:1, and the microbial fertilizer is adsorbed on the zeolite powder to obtain experimental group 2. Each experimental group is packed in a plastic bag and placed at room temperature for 10 days to 10 days. After 15 days, use it for casuarina seedlings, 25g of every seedling. Measure seedling height when growing for 6 months, root nodule number, above-ground part dry weight, underground part dry weight and total dry weight, and do not use any fertilizer (control group 1), use the microbial fertilizer of liquid (control group 2), only Use organic fertilizer (control group 3), only use zeolite powder (control group 4)) to carry out contrast. The results are shown in Table...

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Abstract

The invention relates to microbial fertilizer specially used for casuarinas. The invention is characterized in that the invention comprises liquid bacterial fertilizer which is obtained by mixing the fermentation broth of a Bacillus megaterium de Bary (Bacillus megaterium de Bary), a fermentation broth of a Frankia.sp (Frankia.sp) and stable effect promoter in a volume ratio of 1 to 1.5: 1.5 to 2.0: 0.1-0.5. The components of the stable effect promoter are counted by a mass fraction: 0.01 to 0.05 percent of flavonoids, 0.001 to 0.01 percent of coumarin, 0.001 to 0.01 percent of sodium molybdate, 0.001 to 0.01 percent of cobalt sulfate; the rest is water. The stable effect promoter also comprises a zeolite powder and organic fertilizer. The fertilizer can promote the growth of the casuarinas and increase the survival ratio and preservation ratio of the casuarinas as well as increasing the quantity of the casuarinas seedling with a nodule. Therefore, the resisting performance and the drought-resistance ability of the casuarinas are promoted. The crown closure speed of the plantation is increased. The windbreak and sand fixation effect of the casuarinas in the coastal protection forest is strengthened. The strain CCTCC M 207197 of Frankia.sp fgc6 provided by the invention has a better effect.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to a microbial fertilizer specially used for casuarina. Background technique [0002] The coastal provinces and districts in southern China have a coastline of 7230km, accounting for about 2 / 5 of the total length of the national coastline. The coastal protection forest is known as the "Green Great Wall". It is one of the important ecological forestry projects in China and the first to resist natural disasters such as typhoons The barrier, in which Casuarina (Casuarina) is the main tree species of shelterbelts, since the late 1950s, the 300,000 hectares of Casuarina shelterbelts built in these areas have fundamentally improved the ecological environment of coastal areas, and have played an important role in wind and sand fixation, sea tide and It has unique functions in resisting severe natural disasters such as typhoons and storm surges, and has exerted huge ecological, social and economic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05F11/08
Inventor 康丽华江业根马海宾王胜坤许松葵彭耀强
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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