Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gibberella intermedia and application thereof

A strain and utilization rate technology, applied in the fields of application, fungi, biochemical equipment and methods, etc., can solve the problems of few research reports, insufficient number and types of phosphorus-solubilizing fungi, etc.

Inactive Publication Date: 2017-07-21
青岛秾贝尔生物工程有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gibberella intermedia It belongs to Gibberella genus, and there are few relevant domestic research reports
Phosphorus solubilizing fungi are far less than phosphate solubilizing bacteria in terms of quantity and species, but it is reported that phosphate solubilizing fungi generally have stronger phosphorus solubilizing ability than bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gibberella intermedia and application thereof
  • Gibberella intermedia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Strains Gibberella intermedia Isolation and identification of QJP2

[0020] (1) Isolation of strains

[0021] Strains of the invention Gibberella intermedia QJP2 is obtained from the soil by means of plate dilution coating and scribing. Separation method: (1) Medium: Mengjina inorganic phosphorus solid medium; (2) Soil sample collection: The collection location is Jiaozhou Bay Base, Qingdao Peng wetland, using the 5-point sampling method; (3) Separation step: Weigh 1 g of soil sample in 100 mL of sterile water, place in a shaker at 30°C, 150 r / min for 10 min, and dilute to 10 -2 、10 -3 、10 -4 concentration. Pipette 100 μL of the above diluted solution, and evenly spread it on the surface of Montina inorganic phosphorus solid medium plate, apply three parallels for each gradient, and incubate at 30°C for 7 days, observe whether there is a phosphorus-dissolving circle, according to the diameter of the phosphorus-dissolving circle (D) The ratio of the ...

Embodiment 2

[0028] Example 2: Strains Gibberella intermedia Scale fermentation of QJP2

[0029] (1) Strain activation: strain Gibberella intermedia QJP2 was stored on the slant of PDA medium at -20°C. Before use, the strain was inoculated on the surface of PDA medium by streaking, and activated twice for later use.

[0030] (2) Seed solution preparation: the activated strain Gibberella intermedia Inoculate QJP2 into a Erlenmeyer flask filled with PDB medium, and culture with shaking at 30°C and 120r / min for 3-5 days;

[0031] The PDB medium is: 200 g of potatoes, 20 g of glucose, 1000 mL of distilled water, and the pH is adjusted to 7.0;

[0032] (3) Preparation of strains for solid production: inoculate the seed solution prepared in step (2) into solid medium at a mass ratio of 10%, and incubate at a constant temperature of 28-30°C for 5-7 days;

[0033] The solid medium is: solid material and inorganic salt solution are mixed at a mass ratio of 1:1.8; the solid material is ri...

Embodiment 3

[0035] Example 3: Strains Gibberella intermedia Cell trial of QJP2

[0036] Test material: strain Gibberella intermedia The fermented bacteria powder of QJP2 has the effect of dissolving phosphorus, and the effective number of viable bacteria is greater than 2×10 8 cfu / g.

[0037] Test period: April 2016 to October 2016

[0038] Test location: Pilot test base of Shandong Academy of Forestry

[0039] Test variety: Cotton (Lumianyan No. 28)

[0040] Test soil: fluvo-aquic soil

[0041] Experimental design: Seed dressing in clear water was used as a control, and bacterial powder liquid seed dressing with different dilutions (10 times, 20 times, 30 times) was used as a demonstration. Each treatment was performed in three parallels, and the area of ​​the plot was 20 m 2 , randomly distributed.

[0042] Fertilization amount and fertilization method: Before sowing, use different dilutions (10 times, 20 times, 30 times) of bacterial powder liquid seed dressing, with clear wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Gibberella intermedia and an application thereof, and belongs to the technical field of phosphate-dissolving microorganism fertilizers. The strain is collected in China General Microbiological Culture Collection Center on December 9th, 2016, and the collection number is CGMCC No. 13199. The invention further discloses a microbial agent comprising the strain and a preparation method of the microbial agent. Experiments indicate that the strain has the advantages of high growth speed and strong phosphate-dissolving capacity. The microbial agent prepared from the strain can increase phosphorous fertilizer utilization rate and improve soil structure, and further crop yield increasing effects are achieved.

Description

technical field [0001] The invention belongs to the field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a strain Gibberella intermedia and its uses. Background technique [0002] Phosphorus is an essential nutrient element in the process of plant growth and metabolism. According to statistics, about 75% of the cultivated land in my country is deficient in phosphorus, and more than 90% of the phosphorus in the soil cannot be directly absorbed and utilized by plants. At the same time, the utilization rate of phosphorus fertilizer after application is low. The reason is that a considerable part of phosphorus and Ca in the soil 2+ , Fe 2+ , Fe 3+ with A1 3+ And other metal ions combine to form insoluble phosphate, which is difficult to be absorbed and utilized by crops. The insoluble phosphate in the soil can only be used by crops when the mineralization is differentiated and decomposed to gradually release phosphorus und...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C09K17/14C12R1/645C09K101/00
CPCC09K17/14C09K2101/00C12N1/14C12N1/145C12R2001/645
Inventor 杜丰玉肖琳周远明牛赡光王学军
Owner 青岛秾贝尔生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com