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Bacillus and application thereof

A strain and utilization technology, applied in the direction of application, bacteria, biochemical equipment and methods, etc.

Active Publication Date: 2017-07-07
青岛秾贝尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain Bacillus horneckiae belongs to the genus Bacillus, and there are rare related reports in China

Method used

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  • Bacillus and application thereof
  • Bacillus and application thereof
  • Bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation and identification of bacterial strain Bacillus horneckiae DSM23495

[0021] (1) Strain isolation

[0022] The bacterial strain Bacillus horneckiae DSM23495 of the present invention is obtained from the soil by means of plate dilution coating and streaking. The separation method: (1) medium: Montina inorganic phosphorus solid medium; (2) soil sample collection: collection The location is the Suaeda salsa wetland in Jiaozhou Bay, Qingdao, and the 5-point sampling method is adopted; (3) Separation step: Weigh 1 g of soil sample into 100 mL of sterile water, place it in a shaker at 30°C and 150 r / min for 10 minutes, and successively diluted to 10 -2 、10 -3 、10 -4 concentration. Pipette 100 μL of the above diluted solution, and evenly spread it on the surface of Montina inorganic phosphorus solid medium plate, apply three parallels for each gradient, culture at 30°C for 7 days, observe whether there is a phosphorus-dissolving circle, according to th...

Embodiment 2

[0029] Embodiment 2: Determination of phosphorus dissolving ability

[0030] (1) The phosphate solubilizing ability of phosphate solubilizing bacteria was determined by Montina inorganic phosphorus liquid medium.

[0031] Mengkina inorganic phosphorus liquid medium formula: glucose 10g, (NH 4 ) 2 SO 4 0.5 g, NaCl 0.3 g, KCl 0.3 g, FeSO 4 ·7H 2 O 0.03 g, MgSO 4 ·7H 2 O 0.3 g, MnSO 4 4H 2 O 0.03 g, Ca 3 (PO 3 ) 2 10 g, 1000 mL of distilled water, adjusted to pH 7.0, can be used to quantitatively determine the phosphorus-solubilizing ability of phosphorus-solubilizing microorganisms.

[0032] The strain Bacillus horneckiae DSM23495 preserved on the slant was inoculated into NA liquid medium, and shaken at 30°C and 180 r / min for 12 h to obtain seed liquid; solution was inoculated into a Erlenmeyer flask containing 100 mL Montina inorganic phosphorus liquid medium; and according to the same inoculum amount, sterile NA liquid medium was inoculated into Montina inorganic...

Embodiment 3

[0036] Example 3: Liquid scale fermentation of strain Bacillus horneckiae DSM23495

[0037] (1) Strain activation: the strain Bacillus horneckiae DSM23495 was stored on the slant of NA medium at -20°C. Before use, the strain was inoculated on the surface of NA medium by streaking, and activated twice for later use.

[0038] (2) Seed shaker flask: inoculate the activated strain Bacillus horneckiae DSM23495 into a conical flask containing NA liquid medium, and incubate with shaking at 30°C and 200 r / min for 12 h;

[0039] (3) Seed tank fermentation: Inoculate the shake flask seed solution prepared in step (1) into the seed tank, and ferment until the bacteria enter the logarithmic growth phase;

[0040] Seed tank liquid fermentation medium, formula: 15 g corn flour, 4 g glucose, 21 g bean cake powder, 4 g fish meal, CaCO 3 9g, (NH 4 ) 2 SO 4 1.5 g, K 2 HPO 4 0.5 g, MgSO 4 ·7H 2 O 0.1 g, NaCl 0.1 g, water 1000 mL, pH adjusted to 7.5.

[0041] Fermentation conditions: t...

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Abstract

The invention discloses a Bacillus horneckae and application thereof and belongs to the technical field of phosphate solubilizing microbial fertilizers. The strain has been already preserved in CGMCC, with the preservation data of 9th, January, 2017 and the preservation number of CGMCC No. 13551. The invention also discloses a microbial agent containing the strain and a preparation method thereof. Experiments verify that the strain has the characteristics of being high in growing speed, strong in phosphate solubilizing ability, fast to plant at plant rhizospheres and the like. The microbial agent prepared by the strain has the phosphate solubilizing function and can improve the soil structure, so that the utilization rate of a phosphate fertilizer is favorably increased, and therefore, the microbial agent is of great significance in improving the output and quality of crops.

Description

technical field [0001] The invention belongs to the field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a phosphorus-dissolving Bacillus horneckiae strain and its use, and a phosphorus-dissolving microbial bacterial agent prepared by using the Bacillus horneckiae. Background technique [0002] As one of the three major nutritional elements necessary for plant growth and development, phosphorus is the basic element of key components such as DNA and ATP in plant cells, and participates in photosynthesis and biochemical metabolism of plants at the same time. Therefore, phosphorus is one of the most important nutrient elements in crop planting and production, but at the same time phosphorus is also a resource that is difficult to regenerate in the soil. According to statistics, about 2 / 3 of the cultivated land in my country lacks phosphorus resources, and more than 95% of phosphorus and Ca 2+ , Fe 2+ , Fe 3+ with A1 3+ And ot...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/20C12N1/205C12R2001/07
Inventor 杜丰玉牛赡光肖琳周远明王学军
Owner 青岛秾贝尔生物工程有限公司
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