31 results about "Peronophythora litchii" patented technology

The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition which takes zhongshengrnycin and copper oxychloride as effective ingredients, has an obvious and enlarged bacteriocidal spectrum and no phytotoxicity, and is used for treating plant bacterial diseases as well as fungal diseases. The weight proportion of the zhongshengrnycin to the copper oxychloride is 1:5-60. The bactericide composition has obvious synergistic effect, high field prevention effect and a wide bacteriocidal spectrum, is used for preventing and treating cucumber bacterial angular leaf spot, cucumber downy mildew, tomato bacterial macula disease, tomato leaf mold, pumpkin soft rot, watermelon bacterial angular leaf spot, watermelon sponge, apple alternaria leaf spot, apple brown blotch, pear scabs, grape downy mildew, grape powdery mildew, anthracnose of grape, jujube rust diseases, jujube anthracnose, citrus canker, citrus scab, citrus anthracnose, peanut leaf spot diseases and peronophythora litchii, and has the advantages of good effect and reduction of prevention and treatment cost.

The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.

The invention discloses peronophythora litchii molecular detection primers and a detection method thereof, and is dedicated to specific molecular detection of peronophythora litchi. A pair of peronophythora litchii specific primers PvYF1:5'-GTCCGAGTTTCTAGCAGATTG-3' and PvYR1:5'-ACGATAATACCGTGAGCGCC-3' are mainly designed; through PCR amplification and agarose gel electrophoresis, a specific amplification product with the fragment length of 249 bp is specifically amplified in peronophythora litchii pure DNA and peronophythora litchii-carried plants. The specific molecular detection primers and a usage method thereof can be used for rapid, sensitive and specific detection of peronophythora in the peronophythora litchii infected plants in production practice, at the same time, can be used for early diagnosis of field diseases and monitoring and identification of pathogenic bacteria, and provides a reliable technology and theoretical basis for diseases caused by peronophythora litchii.

The invention discloses a litchi pest monitoring and early warning method and system based on the Internet of Things and a storage medium, and the method comprises the steps: carrying out the image segmentation through obtaining the image information of a litchi plant, and obtaining the image information of a leaf and fruit region; preprocessing the leaf and fruit region image information, constructing a litchi pest recognition model based on a generative adversarial network and a convolutional neural network, importing the preprocessed leaf and fruit region image information into the litchi pest recognition model, and performing litchi pest recognition and classification; and according to the pest recognition result and the main pest state information of the pest, carrying out graded early warning on the litchi pest, meanwhile, according to the litchi pest type information and the main pest state information, formulating a comprehensive prevention and control scheme, and displaying the litchi pest recognition result, the pest early warning and the comprehensive prevention and control scheme according to a preset mode. Classification early warning is carried out through identification of litchi insect pests, a targeted prevention and control scheme of the litchi insect pests is formulated, and high yield and high quality of litchis are guaranteed.

The invention discloses a composition for treating peronophythora litchii based on guaiacol and an application method thereof. Guaiacol is applied to the agricultural field and prepared into the composition together with dimethomorph according to a certain proportion to be used for treating peronophythora litchii. The new application of guaiacol is developed, the peronophythora litchii can be effectively treated, disease resistance of crops is improved, and compared with existing other single pesticides, the composition is better in control effect, low in production cost and worth popularizing.

The invention belongs to the technical field of plant disease biocontrol, and discloses a strain of bacillus altitudinis XJGS-4 and an application thereof in prevention and treatment of peronophythoralitchii. The bacillus altitudinis is named as bacillus altitudinis XJGS-4, is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) at Guangdong Institute of Microbiology, 5 floor, No.59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou City, has the preservation number of GDMCC No:60698, and is preserved on June 17, 2019. The obtained bacillus altitudinis comes from rhizosphere soil of healthy litchi chinensis sonn fruit trees, is mutually symbiotic with litchi chinensis sonn for a long time, and avoids the possibility of damaging of the stability of a micro ecological environment on a plant surface; the bacillus altitudinis and a biological preparation based on the bacillus altitudinis have a strong inhibitory effect on peronophythora litchii, and have agood prospect of development and application.

The invention discloses application of mycophenolic acid in inhibiting Peronophythora litchii. It is concluded through reaches for the first time that mycophenolic acid has an important use in preventing and treating Peronophythora litchii, particularly inhibiting growth of mycelia of Peronophythora litchii and emergence of sporangium of Peronophythora litchii, thereby effectively inhibiting occurrence of Peronophythora litchii. In addition, mycophenolic acid can inhibit the growth of pathogens but can never completely kill them, and it is indicated that mycophenolic acid has low toxicity andis more environmentally friendly. Therefore, the mycophenolic acid has a promising application prospect in preventing and treating Peronophythora litchii.

The invention discloses a peronophythora litchii secreting type protein elicitor PlPeL8 and an application thereof. The amino acid sequence of the protein elicitor PlPeL8 is as shown in SEQ ID NO.1, the coding gene of the protein elicitor PlPeL8 is constructed into a plant expression vector, then agrobacterium tumefaciens is transformed, transient expression is carried out in nicotiana benthamiana cells, and it is found that the protein elicitor PlPeL8 can induce plant defense reactions including causing plant cell allergic necrosis, inducing plant cell apoptosis and inducing plant cell apoptosis. The gene can induce active oxygen bursting and up-regulated expression of marker genes of plant immune related pathways such as salicylic acid and jasmonic acid pathways, can significantly improve the resistance of plants to phytophthora, provides a new way for improving plant disease resistance and reducing diseases, and has a wide application prospect in green development of modern agriculture.

The invention provides a waltheria indica micro-emulsion as well as a preparation method and application thereof. The micro-emulsion is prepared from the following raw materials: waltheria indica extract, an organic solvent, an emulsifier and water, wherein the waltheria indica extract is prepared by crushing the overground part of waltheria indica and conducting extracting with ethyl acetate and methanol; the organic solvent is a mixture of ethyl acetate, ethanol and methanol in a mass ratio of 1: (0.8-1.2): (4-6); the emulsifier is a mixture of castor oil polyoxyethylene ether 40, oleic acid polyoxyethylene ester and lauryl amine polyoxyethylene ether in a mass ratio of 2: (0.8-1.2): (0.8-1.2); and a mass ratio of the waltheria indica extract to the organic solvent to the emulsifier to the water is 1: (1.9-2.1): (1.9-2.1): (14.8-15.2). The waltheria indica microemulsion prepared by the invention has a prevention effect of 94% or more on peronophythora litchii, and can significantly inhibit the deterioration of peronophythora litchii.

A disclosed sterilization composition containing cyazofamid and a strobilurin compound comprises a component A and a component B with a weight ratio of 1%-50%:1%-50%, wherein the active component A is cyazofamid, and the active component B is any one bactericides selected from azoxystrobin, kresoxim-methyl, ZJ0712 (2-[3-(2,5-diMethyl-phenoxymethyl)-phenyl]-3-methoxy-acrylic acid methyl ester), picoxystrobin and enestroburin. The composition provided by the invention is capable of controlling multiple crop diseases, has obvious synergic effect, is enlarged in sterilization spectrum, and has relatively high activity on downy mildew, peronophythora litchii Chen ex Ko et al., eqpidemic disease, anthracnose and powdery mildew; and the pesticide usage amount is reduced, the residual quantity of the pesticide on crops is reduced, and the environmental pollution is mitigated.

The invention discloses a plant immune activator protein PlAvh23 secreted by peronophythora litchii and an application of the plant immune activator protein PlAvh23. A novel peronophythora litchii RXLR type effect protein PlAvh23 is identified, and the amino acid sequence of the novel peronophythora litchii RXLR type effect protein PlAvh23 is selected from any one of the following sequences: (a) an amino acid sequence as shown in SEQ ID NO: 1; (b) an amino acid sequence as shown in SEQ ID NO: 4; and (c) an amino acid sequence as shown in SEQ ID NO: 6. The plant immune activator protein PlAvh23 can induce plants to generate immune response, reduces infection of pathogenic bacteria, provides a new way for improving plant resistance, and can be applied to prevention and treatment of litchi downy blight.

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.

The invention discloses an application of mustard in preparing a fruit preservative. Through the research of the invention, fresh mustard powder of 'Dilong No.1' can effectively inhibit growth of pathogenic bacteria such as botrytis cinerea, peronophythora litchii and the like, and can effectively prolong the storage time of fruits such as apples, juicy peaches, cherries, strawberries, grapes, oranges, mulberries, litchis and the like. The invention provides a feasible technical approach for fruit preservation, and has important significance for maintaining post-harvest quality of fruits, reducing storage loss and promoting sustainable development of the industry.

The invention provides application of a gene PlRACK1 in regulation and control of growth, oxidation resistance and pathogenicity of peronophythora litchii. Experiments prove that upstream and downstream sequences of a gene PlRACK1 are amplified respectively, then an NPT II gene sequence is amplified and connected with a PBSSK carrier, and after homologous recombination, knockout is carried out through a polyethylene glycol mediated protoplast transformation technology and a CRISPR / Cas9 knockout strategy. Due to deletion of the gene PlRACK1, compared with a wild type, the obtained mutant has the advantages that the growth rate of peronophythora litchii is obviously slowed down, the pathogenicity of the peronophythora litchii is obviously reduced, and the tolerance of the peronophythora litchii to oxidative stress is obviously reduced. The invention proves that the gene PlRACK1 is necessary for the growth, development and pathogenicity of peronophythora litchii. The research is helpful for deeply illuminating the pathogenic molecular mechanism of peronophythora litchii, and a target gene is provided for developing effective bactericides.

The invention discloses a germicide composition containing coumoxystrobin, which comprises effective active ingredients consisting of a first active ingredient and a second active ingredient, wherein the first active ingredient is coumoxystrobin, the second active ingredient is a bactericide preventing and treating oomycete diseases and selected from dimethomorph, metalaxyl, metalaxyl-M, benalaxyl, propamocarb, fosetyl aluminium, cymoxanil, cyazofamid or fluopicolide, and the weight ratio of the first active ingredient to the second active ingredient is 1:20-20:1. The composition can be prepared into water dispersible granules, wettable powder, suspending agents and flowable concentrates for seed coating permitted in agriculture. The composition disclosed by the invention has reasonable components, a good bactericidal effect and low medication cost, and the activity and the bactericidal effect of the composition are not the simple sum of the activity of each component. Compared with the existing single preparation, the composition also has an obvious synergistic effect in addition to an obvious sterilizing effect, the generation of resistance is retarded, the residual quantity of the preparation is small, and the composition has no pollution on crops, has good safety and meets the requirement on the safety of the pesticide preparation. The germicide composition disclosed by the invention has obvious prevention and treatment effects on vegetable downy mildew, vegetable epidemics, vegetable and cotton seedling damping off, fruit tree downy mildew and peronophythora litchii.

The invention discloses a comprehensive prevention and control method for peronophythora litchii, which is characterized in that four prevention and control measures of early disease monitoring, agricultural prevention and control, chemical prevention and control and biological prevention and control are integrated and assembled for the peronophythora litchii aiming at four important growth stages of a spring shoot stage, a flower bud stage, a young fruit stage and a later fruit development stage of litchis. And a comprehensive prevention and control measure suitable for the peronophythora litchii at each growth stage is constructed. According to the invention, the modern molecular biological technology PCR is used for monitoring and then guiding prevention and control, so that the occurrence of the litchi peronophythora blight is more effectively prevented and controlled, the prevention and control effect is more obvious, the comprehensive prevention effect on the litchi peronophythora blight is more than 90%, and the yield and quality of litchis are improved. According to the method, measures such as disease monitoring, agricultural prevention and control, chemical prevention and control and biological prevention and control are comprehensively adopted, the litchi peronophythora blight is effectively prevented and controlled, the use frequency and the use amount of pesticides are remarkably reduced, and the method has great significance in litchi industry development and environmental protection.

The invention provides a method for simply, conveniently and rapidly separating peronophythora litchii. The method comprises the following steps: (1) selecting and disinfecting disease tissues of litchi; (2) preparing a V8 vegetable culture medium, adding benzyl ampicillin, prochloraz, hymexazol and a growth promoting agent, and mixing evenly to obtain a selective culture medium, wherein the growth promoting agent is formed by mixing beta-sitosterol mother liquor, litchi rind ground-out liquid and water; (3) culturing the disease tissues in the selective culture medium, selecting hyphae, conducting microscopic examination, and inoculating the hyphae to a new selective culture medium for culturing; and (4) cutting hypha blocks from a microorganism colony, adding the litchi rind ground-out liquid for soaking, and culturing in a new V8 vegetable culture medium to obtain peronophythora litchii. The peronophythora litchii obtained through separation has good activity and pathogenicity. The method is easy to operate, has a high separation rate, is easy for separation, and can realize efficient separation of the peronophythora litchii.

The invention discloses a primer and probe combination for detecting peronophythora litchii based on an RPA lateral flow chromatography technology and a detection method of peronophythora litchii andbelongs to the technical field of biology. The invention discloses a primer and probe combination for detecting peronophythora litchii based on an RPA lateral flow chromatography technology. The primer and probe combination comprises two primers PlRPA-F, a biotin-labeled PlRPA-R and a probe PlRPALFD-P, wherein the RPA detection primer and probe combination is subjected to constant-temperature amplification at 40 DEG C and then is detected by a lateral flow chromatography test strip, and the test strip can be observed to present a positive strip in a detection area. The RPA lateral flow chromatography technology detection primer and probe combination and the detection method thereof disclosed by the invention can be used for rapidly, sensitively and accurately detecting peronophythora litchii infected plants and fruits in production practice, and can also be used for early diagnosis of field diseases and monitoring and identification of germs; reliable technical and theoretical bases are provided for prevention and treatment of diseases caused by peronophythora litchii.

The invention provides a method for inducing peronophythora litchii sporangia to release zoospores. The method comprises the following steps of generation of the peronophythora litchii sporangia, induced generation of zoosporangia, centralized release of zoospores, collection of the zoospores, preparation of a nutrient solution and setting of a cultivation environment. According to the method, the optimum conditions for inducing peronophythora litchii to generate the sporangia and intensively releasing the zoospores can be achieved, the induction of a large number of zoosporangia and the centralized release of the zoospores can be achieved in a short time, the obtained sporangia are large in forming amount, the zoospores are high in release rate and good in germination rate, the problem that the zoospores are difficult to intensively release in a common method is solved, and the problem that zoosporangia generated by induction of a water culture method are easy to pollute is also avoided.

The invention discloses a method for detecting litchi peronophythora blight resistance of litchis and application of the method, and the method comprises the following steps: inoculating peronophythora blight spore suspension on each fresh leaf of the litchis; culturing the inoculated fresh litchi leaves at a constant temperature for 2-4 days; and detecting whether the litchi resists the peronophythora litchii or not according to the disease index of the fresh litchi leaves infected by the peronophythora litchii. The detection method disclosed by the invention is accurate and reliable, can be used for carrying out peronophythora litchii resistance phenotype detection on litchi materials in a large scale in multiple periods in one year, is short in time consumption and low in cost, and can be widely applied to disease-resistant breeding of litchis.

A disclosed sterilization composition containing cyazofamid and a strobilurin compound comprises a component A and a component B with a weight ratio of 1%-50%:1%-50%, wherein the active component A is cyazofamid, and the active component B is any one bactericides selected from azoxystrobin, kresoxim-methyl, ZJ0712 (2-[3-(2,5-diMethyl-phenoxymethyl)-phenyl]-3-methoxy-acrylic acid methyl ester), picoxystrobin and enestroburin. The composition provided by the invention is capable of controlling multiple crop diseases, has obvious synergic effect, is enlarged in sterilization spectrum, and has relatively high activity on downy mildew, peronophythora litchii Chen ex Ko et al., eqpidemic disease, anthracnose and powdery mildew; and the pesticide usage amount is reduced, the residual quantity of the pesticide on crops is reduced, and the environmental pollution is mitigated.

The invention discloses a monitoring and early warning method, system and storage medium for litchi pests based on the Internet of Things. By acquiring litchi plant image information, performing image segmentation to obtain leaf and fruit area image information; processing, constructing a litchi pest recognition model based on generative confrontation network and convolutional neural network, and importing the preprocessed leaf and fruit area image information into the litchi pest recognition model to identify and classify litchi pests; According to the main insect status information of litchi, the classification and early warning of litchi pests are carried out, and at the same time, the comprehensive prevention and control plan is formulated according to the litchi pest type information and the main insect status information, and the litchi pest identification results, pest early warning and comprehensive prevention and control plan are displayed according to the preset method. The invention guarantees the high yield and high quality of the litchi by carrying out graded early warning on the identification of the litchi pests and formulating a targeted prevention and control scheme for the litchi pests.

The invention discloses peronophythora litchii molecular detection primers and a detection method thereof, and is dedicated to specific molecular detection of peronophythora litchi. A pair of peronophythora litchii specific primers PvYF1:5'-GTCCGAGTTTCTAGCAGATTG-3' and PvYR1:5'-ACGATAATACCGTGAGCGCC-3' are mainly designed; through PCR amplification and agarose gel electrophoresis, a specific amplification product with the fragment length of 249 bp is specifically amplified in peronophythora litchii pure DNA and peronophythora litchii-carried plants. The specific molecular detection primers and a usage method thereof can be used for rapid, sensitive and specific detection of peronophythora in the peronophythora litchii infected plants in production practice, at the same time, can be used for early diagnosis of field diseases and monitoring and identification of pathogenic bacteria, and provides a reliable technology and theoretical basis for diseases caused by peronophythora litchii.