The invention discloses an embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast. The embedding-dehydrating ultralow temperature storage method comprises the following steps of: collecting mature
thallus of the scytosiphon lomentaria, and culturing until brown mitoplast is used as an embedding material; crushing the mitoplast to uniformly mix with
sodium alginate liquor containing
sucrose and
glycerol; dropping NaCl liquor to form rubber balls, and hardening to complete an
embedding process; pre-culturing the immobilized rubber balls; placing the rubber balls in a culture dish, placing the culture dish in a large culture dish paved with
drying silica gel on bottom, sealing, and immediately placing in an
incubator of 21 DEG C for
drying and dehydrating under a dark condition; placing the dehydrated rubber balls into a freezing tube, and immediately adding into
liquid nitrogen for storage after sealing. The embedding-dehydrating ultralow temperature storage method for scytosiphon lomentaria mitoplast disclosed by the invention effectively solves problems such as variation and
pollution in a scytosiphon lomentaria seed storage process; the scytosiphon lomentaria mitoplast has complete growth and development ability and amplification ability after being recovered from freezing, and can form normal
sporangium and release spores, so that
thallus is developed, a dear apparatus is not needed, and an anti-freezing protective agent with toxic action to the stored material is also not needed to be used. Therefore, popularization and application of the method are facilitated.