Method for generating sporangia and releasing zoospore by inducing phytophthora capsici

Abstract

The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g / L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a method for inducing Phytophthora capsici to produce sporangia and release zoospores. Background technique [0002] Pepper blight is a devastating fungal disease that occurs worldwide. It was first discovered in New Mexico, USA in 1918. Phytophthora capsici Leonian, which caused pepper blight, was named by Leon Leonian in 1922. It belongs to plant pathogenic oomycetes. Phytophthora capsici overwinters in diseased soil as oospores or chlamydospores, and can survive for several months or even longer. It has a wide range of hosts and mainly infects many important vegetable crops such as peppers, tomatoes, eggplants, cucumbers, and pumpkins. Capsicum blight can occur in both open fields and protected areas. The prevalence of the disease has caused serious losses of peppers. The general rate of diseased plants is 15-30%, and it can reach more than 80% in sev...

AI Technical Summary

Problems solved by technology

It takes more than 10 days for this method to induce Phytophthora to produce sporangia, which is a long time; meanwhile, the soil leachate is not strictly sterilized, and the skin culture medium is sterilized by moist heat, so the effect of inducing zoosporangia is unstable and easy to contaminate

There are other induction methods that can also produce a large number of zoospores: rye culture medium to culture mycelium overgrown the plate, smear the medium plate covered with mycelia with a sterile L-shaped glass rod, and place it at 28°C for light biochemical Cultivate in an incubator for 24 hours to induce the production of sporangia, then add 18 mL of sterile water to the medium plate that has produced sporangia, and then move it to a refrigerator at 4°C for 1 hour to induce Phytophthora capsici to produce zoospores, but the above method It is more cumbersome and needs to be filtered to obtain usable zoospores

In addition, the fresh host plant material induction method can also induce sporangia to produce zoospores after low temperature treatment, but this method is prone to bacterial contamination, and plant materials are difficult to obtain