The present invention relates to a preparation method for microbial polypeptide preparation with the use of lactic acid bacteria and bacillus subtilis, and which is characterized in that the high yield enzyme and high quantity production strains is selected and trough the integration of original microwave-induction and rays-induction, wherein the production process applies the liquid-alone culture and solid-composite culture, adds special product promoter into the medium, the content of soybean peptide is high, at the same time which is rich in metabolic products such as peptidoglycan, microbial antioxidants and enzymes. The present invention has the advantage of low production cost, faster cycle, and faster absorption for animal after breeding, high daily increasing in weight, immunity enhancing, and stress rising, which has obvious effect in breeding and is a safe and high efficient new feed additive.