Application of litsea cubeba essential oil in controlling pepper phytophthora blight
A technology of pepper blight and litsea cubeba, applied in the field of application of litsea cubeba essential oil to control pepper blight, can solve problems such as difficult production and cultivation, human health hazards, and threats to food safety, so as to inhibit the development and spread of diseases, The effect of good protective effect and good therapeutic effect
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Embodiment 1
[0019] Dissolving the litsea cubeba essential oil in acetone, adding Tween-60, and finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentration of Litsea Cubeba essential oil, 0.2 g acetone, and 1 g Tween-60 in each 1 L of medicinal solution.
[0020] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 45°C, extraction time 60 minutes, extraction pressure 2.5 MPa, CO 2 Flow rate 1.5 mL / min.
Embodiment 2
[0022] Dissolving the litsea cubeba essential oil in methanol, adding Tween-80, finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentrations of Litsea Cubeba essential oil, 0.3 g methanol, and 1.5 g Tween-80 were contained in each 1 L of medicinal solution.
[0023] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 40°C, extraction time 80 minutes, extraction pressure 2 MPa, CO 2 The flow rate is 2 mL / min.
Embodiment 3
[0025] Dissolving the litsea cubeba essential oil in ethanol, adding Tween-60, and finally diluting and mixing with sterile water to obtain the medicinal solution of the required concentration of the litsea cubeba essential oil. The concentration of Litsea Cubeba essential oil, 0.4 g of ethanol, and 2 g of Tween-60 in each 1 L of liquid medicine.
[0026] The preparation method of the above-mentioned litsea cubeba essential oil comprises the following steps: drying, grinding and sieving the litsea cubeba fruit to obtain litsea cubeba powder; 2 extraction to obtain litsea cubeba essential oil; among them, supercritical CO 2 Extraction conditions: extraction temperature 50°C, extraction time 40 minutes, extraction pressure 3 MPa, CO 2 The flow rate is 1.2 mL / min.
[0027] The liquid medicine prepared in Examples 1-3 is applied to the crops by spraying the whole plant.
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