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Introduction method for sea-tangle

A technology of kelp and seawater, applied in botany equipment and methods, seaweed cultivation, plant cells, etc., can solve the problems of large size, failure of introduction, spore release and death, etc., and achieve high ability to resist harsh environment and high success rate Effect

Inactive Publication Date: 2008-02-13
MARICULTURE INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for introducing kelp, which can solve the problems in the prior art of selecting algae fragments with mature sporangia in the leaves and transporting them over long distances, which is too bulky to carry, the leaves of the algae are prone to rot, and the spores disperse and die halfway. , leading to problems such as the failure of introduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) In the introduced sea area, when the seawater temperature reaches above 16°C, collect the kelp leaves with mature sporangia;

[0022] (2) Transport it indoors, wash the kelp leaves with mature sporangia with seawater, dry them in the shade and stimulate them for 60 minutes, put them into a container filled with sterilized seawater, control the water temperature at 8-12°C, and let the spores disperse;

[0023] (3) When the density of zoospores in the seawater under microscope inspection is 10 / 100X, filter the spore water with gauze, filter out the mucus and sundries, and put it into a brown curtain attached seedling device and a glass slide;

[0024] (4) The spore attachment density is controlled at 30-60 / 100X, and the attached seedling device and slides after the spores are attached are put into a temperature-controlled light incubator to cultivate, and the light intensity is controlled at 1000lx, and the light is controlled for 12 hours every day, and the water temp...

Embodiment 2

[0030] (1) In the introduced sea area, when the seawater temperature reaches above 16°C, collect the kelp leaves with mature sporangia;

[0031] (2) Transport it indoors, wash the kelp leaves with mature sporangia with seawater, dry them in the shade and stimulate them for 50 minutes, put them into a container filled with sterilized seawater, control the water temperature at 8-12°C, and let the spores disperse;

[0032] (3) When the microspore density in the seawater is 5 / 100X, filter the spore water with gauze, filter out the mucus and sundries, and put it into the palm curtain attached seedling device;

[0033] (4) The spore attachment density is controlled at 30-60 / 100X, and the attached seedling device after the spore attachment is placed in a temperature-controlled light incubator for cultivation. The light intensity is controlled at 800lx, and the light is controlled for 14 hours a day. The water temperature is controlled at 9.0°C , after cultivating for 8 days, gametoph...

Embodiment 3

[0039] (1) In the introduced sea area, when the seawater temperature reaches above 17°C, collect the kelp leaves with mature sporangia;

[0040] (2) Transport it indoors, wash the kelp leaves with mature sporangia with seawater, dry them in the shade and stimulate them for 70 minutes, put them into a container filled with sterilized seawater, control the water temperature at 8-12°C, and let the spores disperse;

[0041] (3) When the microspore density in the seawater is 15 / 100X, filter the spore water with gauze, filter out the mucus and sundries, and put it into the palm curtain attached seedling device;

[0042] (4) The spore attachment density is controlled at 30-60 / 100X, and the attached seedling device after the spore attachment is put into a temperature-controlled light incubator for cultivation, and the light intensity is controlled at 1200lx, and the light is controlled for 10 hours every day, and the water temperature is controlled at 10°C. , after 6 days of cultivati...

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Abstract

The present invention provides an introduction method of the laminaria japonica, which can resolve the problems of the prior art that the selected frond section having the mature sporangia is easy to decay and the spore is easy to release, thereby leading to the failure of the introduction. The introduction method adopted by the present invention is to firstly dry and incite the mature sporangia leaf of the laminaria japonica in a temperature-controllable laboratory, then put the mature sporangia leaf of the laminaria japonica into a water tank that is provided with the fresh sterilized sea water and the temperature of the water is controlled between eight cent degrees and twelve cent degrees to release the spores, then cultivate the spore to form the gametophyte, to put the seedling curtain attached with the gametophyte into a low-temperature enclosed bag and finally transport in low-temperature storage to the destination. The shape of all gametophytes on the laminaria japonica seedling curtain is normal, which has high successful rate and the survival rate is nearly one hundred percentages.

Description

technical field [0001] The invention relates to a method for introducing seaweed, in particular to a method for introducing seaweed. Background technique [0002] Kelp is a large brown algae with high nutritional value. Not only for human consumption, but also as a raw material for food, chemical, pharmaceutical, feed and seaweed industries. Kelp algae contain a variety of plant compounds that are not found in terrestrial vegetables. The kelp polysaccharide contained in it has strong immunity-enhancing and anti-tumor effects. Therefore, the development and utilization of kelp is on the rise all over the world. Since the 1950s, my country has carried out artificial cultivation of kelp, and became the first pillar industry of aquaculture. But for many years, there is only one species of kelp cultured in my country. In the past 50 years, kelp seedlings and kelp culture production have basically been reused. Due to the lack of breeding and rejuvenation technology, the species ...

Claims

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Application Information

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IPC IPC(8): A01G33/00C12N5/04
Inventor 李美真王志刚丁刚詹冬梅于波
Owner MARICULTURE INST OF SHANDONG PROVINCE
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