78results about How to "Morphologically normal" patented technology

The present invention provides an introduction method of the laminaria japonica, which can resolve the problems of the prior art that the selected frond section having the mature sporangia is easy to decay and the spore is easy to release, thereby leading to the failure of the introduction. The introduction method adopted by the present invention is to firstly dry and incite the mature sporangia leaf of the laminaria japonica in a temperature-controllable laboratory, then put the mature sporangia leaf of the laminaria japonica into a water tank that is provided with the fresh sterilized sea water and the temperature of the water is controlled between eight cent degrees and twelve cent degrees to release the spores, then cultivate the spore to form the gametophyte, to put the seedling curtain attached with the gametophyte into a low-temperature enclosed bag and finally transport in low-temperature storage to the destination. The shape of all gametophytes on the laminaria japonica seedling curtain is normal, which has high successful rate and the survival rate is nearly one hundred percentages.

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

The normal temperature and sub-normal temperature in vitro heart preserving perfusate as one kind of organ preserving matter consists of basic electrolyte, energy matter and protecting matter. Each liter of the heart preserving perfusate contains NaCl 5.0-7.0 g, KCl 0.3-0.5 g, NaH2PO4 .H2O 100-150 mg, MgSO4 50-100 mg, NaHCO3 3.0-5.0 g, L-argine 70-100 mg, histidine 30-60 mg, L-cystine 50-70 mg, L-methionine 10-50 mg, levo glutamine 500-700 mg, L-lysine 100-170 mg, glycine 10-50 mg, L-isoleucine 70-150 mg, L-serine 30-50 mg, L-leucine 70-150 mg, L-tryptophane 10-20 mg, L-tyramine 80-150 mg, L-valine 70-150 mg, L-threonine 80-150 mg, choline chloride 3-8 mg, vitamin B2 0.5-3 mg, pantothenic acid 3-g mg, folic acid 3-8 mg, pyridoxal 3-10 mg, nicotinamide 3-10 mg, etc. The present invention has the effects of preserving in vitro heart efficiency, reducing rejection reaction of organ and prolonging the survival period of organ.

The invention provides a method for maintaining the survival and / or function of vascular endothelial cells in the in vitro preservation process and used preserving fluid. The method and the preservingfluid are suitable for storing various organs. The in vitro liver can survive to exceed 24h during the preservation by the organ preserving liquid and in the mechanical filling process; the liver microcirculation structure is complete; no obvious damage exists; the choleresis function is good; the liver synthesis and metabolism functions are good; the in vitro preservation time of the liver is greatly prolonged; the postoperative survival rate of later-period liver transplantation patients is improved.

The invention belongs to the field of biologic medicines and discloses mesenchymal stem cell preservation liquid, and a preparation method and application thereof. The mesenchymal stem cell preservation liquid contains AB cord blood plasma which is 5-15% of the volume of the preservation liquid and low molecular weight heparin calcium which is 70-80 AXaIU / ml. The AB cord blood plasma and mesenchymal stem cells come from the same provider. By means of the mesenchymal stem cell preservation liquid provided by the invention, the activity maintaining time of the mesenchymal stem cells can be prolonged (above 90% of activity is kept at 4-15 DEG C for 72 h); after the mesenchymal stem cells are preserved in the preservation liquid for 72h, the cells are normal in form, the proliferation and amplification capability of the cells cannot be influenced, and the phenotypic characteristics of the mesenchymal stem cells cannot be influenced; the mesenchymal stem cell preservation liquid disclosed by the invention is safe and reliable for clinical application; and, because the AB cord blood plasma and the cord mesenchymal stem cells come from the same placenta and belong to auto-plasma, the possibility of exogenous virus pollution is reduced.

The invention relates to an application of ligustilide in preparing a composition for preventing and treating senile dementia, which comprises ligustilide as an effective component and other components acceptable pharmaceutically and / or acceptable in food or auxiliary components. As required, the effective components can comprise at least one of acetylcholinesterase inhibitor, excitatory amino acid and peptide transmitter components, Abeta fibration and sedimentation disturbance components, antioxidant, microcirculation improving components and calcium antagonist accounting for 10%-40% of the total weight. Shown by experimental results, through the protective actions of the ligustilide on damage caused by oxidation, apoptosis and inflammatory reaction induced by the Abeta amyloid peptide fragment and aging neuron degenerative change, the composition can obviously improve the descending of learning and memory caused by the Abeta amyloid peptide fragment and the aging neuron degenerative change, and can be used as a medicine and / or a health food composition.

The invention relates to a heat treatment method for a U71MnH steel rail flash welding joint, and belongs to the technical field of steel rail welding. The problem that harmful structures such as martensites are generated to influence the joint hardness due to the fact that the local cooling speed is higher than the critical transition temperature when an existing steel rail welding joint is cooled in the heat treatment process is solved. The heat treatment method for the U71MnH steel rail flash welding joint comprises the steps that a U71MnH steel rail welding joint welding seam area which iscooled to 300 DEG C or below after being welded is heated to 870-930 DEG C, then heating is stopped, air spraying cooling is adopted and stopped after the steel rail joint tread temperature is lowered to 420-470 DEG C, and natural cooling is conducted till the room temperature is achieved. By adopting the method, it is guaranteed that the joint structure is normal while the hardness of the U71MnHsteel rail flash welding joint is effectively controlled.

The invention relates to a heat treatment method for a U71MnH steel rail welding joint, and belongs to the technical field of steel rail welding. The problem that harmful structures such as martensites are generated to influence the joint hardness due to the fact that the local cooling speed is higher than the critical transition temperature when an existing steel rail welding joint is cooled in the heat treatment process is solved. The heat treatment method for the U71MnH steel rail welding joint comprises the steps that a U71MnH steel rail welding joint welding seam area which is cooled to 300 DEG C or below after being welded is heated to 915-935 DEG C, then heating is stopped, air spraying cooling is adopted and stopped after the steel rail joint tread temperature is lowered to 405-432DEG C, and natural cooling is conducted till the room temperature is achieved. By adopting the method, it is guaranteed that the joint structure is normal while the hardness of the U71MnH steel railflash welding joint is effectively controlled.

A vitrifying freeze storage method for the embryos of fish, especially the bastard halibut, is disclosed, which features that the formula of vitrifying liquid suitable for the embryo of seawater fish is screened out, the embryo development stage suitable for vitrifying it is determined, a particular eluting solution is disclosed, and the step balance and the fast cooling and thawing techniques are used. Its advantages are high safety, easy operation and low cost.

The invention provides a stabilizing and buffering liquid of low molecular weight sugars used for lyophilization preservation of platelet, which is water solution with 35-45mM thickness by adding thelow molecular weight sugars on the basis of basic buffering liquid composing prescriptions (GS), wherein the basic buffering liquid composing prescriptions (GS) comprises 140mMNaCL, 5Mmkcl,12mM sodiumcitrate, 10mM glucose, 12.5mM sucrose and 1mug / mLPGE1. The low molecular weight sugars are trehalose. After the platelet is stabilized and protected by the stabilizing and buffering liquid of low molecular weight sugars of the invention, the platelet is freeze-dried with rehydration. The recovery ratio of a cell is above 70% and accumulating activity evoked by ADP can achieve 50% of fresh platelet. The platelet freeze-dried with rehydration has normal shape and complete structure, thereby a new solution is provided for freeze-drying conservation of the platelet.

The invention relates to a post weld heat treatment (PWHT) method for flash welding joints of U71MnH rails, and belongs to the technical field of rail welding. The invention provides the PWHT method for the flash welding joints of the U71MnH rails in order to solve the problem that when existing rail welding joints are cooled during heat treatment, the hardness of the joints is affected by martensite and other harmful structures which are formed because local cooling rate is higher than critical transition temperature. The PWHT method comprises the steps of heating U71MnH rail welding joint welding seam areas, which are cooled to be below 300 DEG C after welding, to 919-940 DEG C and then stopping heating, performing air jet cooling, stopping air jet cooling after the temperature of treadsurfaces of the joints of the rails drops to 410-435 DEG C, and performing natural cooling to room temperature. While effectively controlling the hardness of the welding joints of the U71MnH rails, the PWHT method ensures the normality of the structures of the joints.

The invention discloses a novel application of nitrate on an aspect of preventing radiation-induced salivary gland damage, and belongs to the fields of medicine and health. Upon clinical researches and animal experiments, it finally verifies that the nitrate, through oral administration, can be used for effectively preventing the radiation-induced salivary gland damage and for improving the symptom of xerostomia in patients with head and neck tumors due to radiotherapy. The nitrate disclosed by the invention can be used for preparing medicine which is effective on preventing the radiation-induced salivary gland damage; therefore, a novel way is provided for the clinical treatment of radiation-induced xerostomia.

The invention discloses a PyTPS (porphyra yezoensis ueda trehalose-6-phosphate synthase) gene which is cloned to construct a plant expression vector of the gene, and the plant expression vector is transferred into the agrobactrium tumefaciens strain, so that an engineering strain is obtained. A rice variety is transformed by utilizing agrobacterium-mediated transformation, so that genetically modified rice is obtained; after growing and acclimatization, a T0 individual plant is obtained, and a T1 seed is collected; a seedling germinated from the T1-generation genetically modified seed receives PCR (polymerase chain reaction) detection and a salt tolerance assay, so that a T1-generation genetically modified salt-resistant strain is obtained, a T2 generation receives a kanamycin resistance assay, PCR detection and Southern hybridization, and results indicate that the PyTPS gene is already integrated into the genome of the genetically modified strain. The PyTPS gene in the invention has a TPS domain as well as a TPP (thiamine pyrophosphate) domain; the PyTPS gene source is safe; the development of the plant morphology of the PyTPS genetically modified rice is normal; the PyTPS gene can be stably inherited in genetically modified plants; and moreover, the transformation system is highly effective.

The invention relates to a post-welding heat treatment method of U71MnH rail welded joints, and belongs to the technical field of rail welding. The post-welding heat treatment method of the U71MnH rail welded joints aims at solving the problem that the hardness of the joints is affected by harmful tissues such as martensite generated due to the local cooling speed being higher than the critical transition temperature when existing rail welded joints are cooled in the heat treatment process. The post-welding heat treatment method of the U71MnH rail welded joints comprises the following steps ofheating welding seam areas of the U71MnH rail welded joints cooled to below 300 DEG C after being welded to 865-925 DEG C, stopping heating, adopting air spraying for cooling, stopping air spraying for cooling after the temperature of the tread surfaces of the rail joints drops to 458-475 DEG C, and naturally cooling to room temperature. According to the post-welding heat treatment method of theU71MnH rail welded joints, the hardness of the U71MnH rail welded joints is effectively controlled, and meanwhile, normal tissues of the joints is ensured.

The invention provides a reagent capable of preventing and eliminating pollution of mammalian cells, and belongs to the field of microbial pollution control in mammalian cell culture. A polypeptide having a sequence of SEQ ID NO:1 can be used for preventing and eliminating pollution of the mammalian cells; the reagent is composed of the polypeptide having the sequence of SEQ ID NO:1 and amino acid and does not contain any antibiotic component. The product is prepared in accordance with the concentration required for prevention and elimination of pollution, microbial pollution in the mammalian cells can be effectively prevented and eliminated, and the defects of use restriction of antibiotic components in a conventional product and longer cell treatment period are overcome; and the product is good in stability and easy to preserve.

A full-function ring-cutting electric hair follicle transplanting pen comprises a pen head and a tubular pen tip.A motor is mounted in the pen head, the pen tip is driven by the motor to rotate, the tubular pen tip is circumferentially provided with 2-4 rectangular windows uniformly, a window bottom edge of each rectangular window inclines from the outside above to the inside below, an annular sliding sleeve sleeves outside the tubular pen tip in a manner of being capable of sliding up and down, 2-4 claw-shaped blades are integrally connected below the annular sliding sleeve, the bottom edge of each blade forms a sharp cutting edge, each blade correspondingly extends into the corresponding rectangular window, and a vertical pushing mechanism pushing the annular sliding sleeve to move vertically and a vertical positioning mechanism for locating a vertical position of the annular sliding sleeve are further arranged.By using the full-function ring-cutting electric hair follicle transplanting pen, all operation steps from electric hair extracting from a supplying area to electric hair planting from a receiving area can be completed smoothly, and the number of hair of a patient can be multiplied.

The invention discloses a bone repair material able to guide bone tissue regeneration. The material is a composition of calcined bone powder with different particle sizes and a bioabsorbable high polymer material. The bone repair material is characterized in that: the particle sizes of the calcined bone powder range from 0.1-2mm; and the bioabsorbable high polymer material is one or more of collagen, chitin, chitosan, hyaluronic acid, chondroitin sulfate, calcium alginate, fibrous protein, and elastin. The bone repair material provided in the invention has an obvious bone healing guiding effect and good biocompatibility. Also with certain hardness and strength, the bone repair material is easy to shape and has rich sources, thus solving the making problems of artificial synthetic materials in the aspects of porosity, pore traffic and pore size, etc.

The invention relates to a polyploidy induction method for fiber linum usitatissimum under an isolated culture condition, wherein the method comprises the following steps of: selecting health and full fiber linum usitatissimum seeds; sterilizing the seeds and then culturing into seedling; processing the stem tip with colchicines to induce polyploid; transforming into a stem tip extending culture media for culturing; identifying the ploidy of the stem tip chromosome; culturing the stem section which is identified to be a tetraploid plant in a callus and bud induction culture medium to obtain lots of tetraploid sterile seedlings; and then transforming to an extending growing culture medium to grow; performing rooting culture in a rooting culture medium, and then transferring to a sterilized substrate for manging; and finally identifying the ploidy of chromosome of the root tip. The method can obtain higher inductivity than that of a method which processes the fiber linum usitatissimum seeds; the inductivity can be up to 25.5%; the pure tetraploid ratio is high; the polyploidy plant has normal shape, and high transplanting survival rate; the method can realize quick breeding under the isolated condition to obtain a large amount of tetraploid tissue-cultured seedlings in a short time.

The invention discloses a method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes. According to the characteristic of dwarfed plant type of plant transformed and regenerated through the agrobacterium rhizogenes, polyembryony breed of early gold sweet orange is transformed through agrobacterium rhizogenes NSU440, hairy roots are induced, then complete plant is obtained through a way of germination of hairy roots and radication of regeneration buds, and finally a dwarfed breed suitable for labor-saving cultivation is cultivated, so that the fruit garden labor efficiency is improved, and the labor expenditure is reduced. Transformation receptor material adopts early gold sweet orange aseptic seedling epicotyl, and as sweet orange belongs to a polyembryony breed, regeneration plant can keep parental excellent quality.

The invention relates to a traditional Chinese medicament for treating hepatitis and cirrhosis ascites which is prepared from the following raw materials (by weight portion): red ginseng 60-100 parts, white atractylodes rhizome 50-80 parts, borneol 30-50 parts, chicken's gizzard-skin 30-50 parts, red peony root 20-30 parts, blood clam shell 80-100 parts, centipede 40-50 parts, ligumaloes 20-30 parts, earthworm 80-100 parts and polyporus umbellatus 55-80 parts. The medicament is prepared through mixing, grinding into fines, sieving, and can be prepared into powders, tablets, capsules or pills.

The invention specifically relates to a method for acquiring a suspension cell line with the hypocotyl of cabbage type rape as explant and application of the suspension cell line, belonging to the technical field of plant cell culture. According to the invention, the aseptic hypocotyl of cabbage type rape is used as explant and subjected to induction of embryonic callus, culture of suspension cells and the like so as to construct the suspension cell line of the cabbage type rape; and the suspension cell line is used for research on and comparison of the different morphologies and activities ofsuspension cells under the conditions of normal supply of essential micronutrient elements and shortage of the essential micronutrient elements. The prepared suspension cells of the cabbage type rapeare high in activity, stable and uniform in morphology, short in culture period, not limited by natural environmental factors like season and climate, and applicable to research on the cell development of the cabbage type rape at a single cell level and the cell growth, morphology, transmembrane nutrient transport, physiological and biochemical metabolism and gene expression of the cabbage type rape under the condition of abiotic stress.

The invention relates to a culture medium in the technical fields of fermentation engineering and biology, in particular to a nutrition-enhanced culture medium for preparing 2-KGA through fermentation, and the nutrition-enhanced culture medium comprises a conventional culture medium and a nutrition enhancer, wherein sorbitol or sorbose is used as a substrate of the conventional culture medium. Meanwhile, the invention also relates to a method for preparing the 2-KGA by applying the enhanced culture medium. The nutrition-enhanced culture medium can promote the growth of small bacteria, enablesthe small bacteria to be disengaged from the dependence on associated bacteria, establishes independent growth and provides a foundation for the improvement of a 2-KGA fermentation process.

The invention relates to a compound preparation of montmorillonite. The compound preparation is characterized by being prepared by adding a zinc salt into montmorillonite, wherein the weight ratio of the montmorillonite to the zinc salt is 1:(0.0016-0.044). The compound preparation is used for treating diarrhea, peptic ulcer and zinc deficiency, and has the advantages of no side effects, simple production process and good effects for treating diarrhea, peptic ulcer and zinc deficiency.

The invention relates to a traditional Chinese medicine decoction for treating severe acute pancreatitis and belongs to the technical field of traditional Chinese herbal medicine preparations for treating pancreatitis. The traditional Chinese medicine decoction is decocted by nineteen traditional Chinese herbal medicines such as gypsum, rheum officinale, mirabilite and rhizoma anemarrhenae. The traditional Chinese medicine decoction is used for treating the severe acute pancreatitis with the comprehensive synergistic effect of the nineteen traditional Chinese herbal medicines according to the theory of traditional Chinese medical recipe based on the traditional Chinese medicine principle of research and treatment on pathogenesis of severe acute pancreatitis. The traditional Chinese medicine decoction has the functions of clearing heat and removing toxicity, invigorating pulse-beat and defecating feces excretion and regulating qi to remove blood stasis, is few in side effects on the human body, simple in treatment method, low in cost, convenient to take and free of toxic and side effects, and is safe and effective. The whole recipe of the traditional Chinese medicine decoction has the functions of clearing heat and removing toxicity, invigorating pulse-beat and defecating feces excretion, regulating qi to remove blood stasis and treating both symptoms and root causes; and the effective rate of treatment on the severe acute pancreatitis reaches over 87.5%.

The invention relates to the field of economical brown kelp seaweed varietal complexity, in particular to a method for detecting varietal complexity in kelp summer seedling refrigeration water process. The method comprises the steps that a seedling rope which is inoculated with a female gametophyte clone is put in a culture medium or a kelp seedling refrigeration water circulation system to be cultured, parthenogernesis sporophore is obtained when the seedling rope is put in the culture medium, and offspring sporophore is obtained when the seedling rope is put in the kelp seedling refrigeration water circulation system; morphological characteristics and genetype of the offspring sporophore on the seedling rope in the refrigeration water circulation system are detected and compared with those of the parthenogernesis sporophore so as to determine whether varieties are hybrid or not in the seedling process. According to the detection method, it is verified for the first time that in the kelp summer seedling production process, due to the fact that low temperature circulation water is used in common, the varietal complexity is definitely caused.

The invention belongs to the technical field of tissue culture, and relates to a tissue culture and rapid propagation research method suitable for industrialized production. The method is summarized and concluded on the basis of experimental research and production for two years on the basis of a new variety of caladium bicolor introduced by Thai, has strong operability and can be completely used for guiding production. According to the invention, the complete rapid induction, multiplication, rooting and seedling hardening propagation method which has high inductivity and propagation multiple and is suitable for industrial production is established for the first time, a batch rapid propagation situation is formed, and the propagation generations, the hormone concentration of a culture medium and the proportion are controlled to change along with the change of the generations, stability, activity and the proliferation coefficient of seedlings are ensured, and the yield and quality are improved; and according to the method, a production manner of simultaneously propagating and rooting is adopted, so that the production cost is reduced, the production progress is accelerated, the propagation coefficient is ensured, the quality of rooted seedlings is ensured, the method is completely suitable for large-scale industrialized production, and the breeding efficiency of the caladium bicolor is greatly improved.

The invention discloses a design method of a large-curvature cable-membrane structure, and aims to solve the technical problems that the existing cable-membrane structure is easy to generate larger deformation to change the shape size or loosen, and the analysis and design theory of the cable-membrane structure at home and abroad is not completely mature. The design method comprises the following steps: (1) carrying out performance analysis on a membrane material to be used by the large-curvature cable membrane structure; (2) adopting ANSYS software to carry out shape finding analysis on the large-curvature cable membrane structure; (3) selecting a membrane material with a proper thickness; (4) optimizing a reinforcing cable net in the large-curvature cable membrane structure; (5) carrying out technical verification and hardware optimization on the large-curvature cable membrane structure; and (6) designing proper light transmittance of the membrane material. According to the design method, a collaborative stress system of the steel cable, the membrane material, the hardware and the cable membrane support steel structure is optimized, the overall stress stability of the structure and the applicability of the component are improved on the premise that the material performance is brought into full play, and the building body art is perfectly expressed.

The invention belongs to the field of biomedicine and discloses a mesenchymal stem cell preservation solution and its preparation method and application. The mesenchymal stem cell preservation solution contains human AB type umbilical cord blood plasma and 70- 80AXaIU / ml calcium low molecular weight heparin; the human AB type umbilical cord blood plasma and mesenchymal stem cells come from the same provider. The mesenchymal stem cell preservation solution of the present invention can prolong the activity maintenance time of mesenchymal stem cells (maintain more than 90% of the activity for 72 hours at 4-15°C), and the mesenchymal stem cells are stored in the preservation solution of the present invention for 72 hours. , the cell morphology is normal, does not affect its proliferation and expansion ability, and does not affect the phenotypic characteristics of mesenchymal stem cells. The mesenchymal stem cell preservation solution of the present invention is safe and reliable in clinical application, and the AB-type umbilical cord blood plasma and umbilical cord mesenchymal stem cells are derived from the same placenta and belong to autologous plasma, which reduces the possibility of exogenous virus contamination.

The invention discloses an infection enhancing culture medium and method for improving the cell lentivirus infection rate, and relates to the field of MDA-MB-231 cell transfection. The enhanced infection culture medium is used for improving the lentivirus infection rate of the MDA-MB-231 cell, and the enhanced infection culture medium is prepared from the following components: a Leibovitz's L-15 culture medium, an RPMI-1640 culture medium, fetal calf serum, non-essential amino acid and an epidermal growth factor; according to the volume percentage of the total raw materials, the addition amount of the fetal calf serum is 10%, and the addition amount of the non-essential amino acid is 1%; the addition amount of the epidermal growth factor is 2-3ng / ml. By adopting the enhanced infection culture medium disclosed by the invention, the MDA-MB-231 cells can be normally cultured in the conventional environment of 37 DEG C and 5% CO2, and the infection efficiency of the lentivirus on the MDA-MB-231 cells is more remarkably improved, so that the gene editing efficiency of the MDA-MB-231 cells becomes higher, and the problem that the lentivirus infection rate of the MDA-MB-231 cells is low at present is solved.

The invention provides a human chondrocyte culture medium, and belongs to the technical field of cells. The invention optimizes a traditional culture medium, and by adding exosomes, fetalbovine serumand penicillin-streptomycin into a basal culture medium, chondrocyte proliferation can be effectively promoted, dedifferentiation of human chondrocytes is prevented, transformation of the human chondrocytes into fibrocyte-like phenotypes is prevented, and the single-layer culture passage number is increased. The invention also provides a method for isolating culture of the human chondrocytes by using the above-mentioned culture medium, and normal chondrocytes cultured to the sixth passage can be obtained.