78results about How to "Morphologically normal" patented technology

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

The normal temperature and sub-normal temperature in vitro heart preserving perfusate as one kind of organ preserving matter consists of basic electrolyte, energy matter and protecting matter. Each liter of the heart preserving perfusate contains NaCl 5.0-7.0 g, KCl 0.3-0.5 g, NaH2PO4 .H2O 100-150 mg, MgSO4 50-100 mg, NaHCO3 3.0-5.0 g, L-argine 70-100 mg, histidine 30-60 mg, L-cystine 50-70 mg, L-methionine 10-50 mg, levo glutamine 500-700 mg, L-lysine 100-170 mg, glycine 10-50 mg, L-isoleucine 70-150 mg, L-serine 30-50 mg, L-leucine 70-150 mg, L-tryptophane 10-20 mg, L-tyramine 80-150 mg, L-valine 70-150 mg, L-threonine 80-150 mg, choline chloride 3-8 mg, vitamin B2 0.5-3 mg, pantothenic acid 3-g mg, folic acid 3-8 mg, pyridoxal 3-10 mg, nicotinamide 3-10 mg, etc. The present invention has the effects of preserving in vitro heart efficiency, reducing rejection reaction of organ and prolonging the survival period of organ.

The invention belongs to the field of biologic medicines and discloses mesenchymal stem cell preservation liquid, and a preparation method and application thereof. The mesenchymal stem cell preservation liquid contains AB cord blood plasma which is 5-15% of the volume of the preservation liquid and low molecular weight heparin calcium which is 70-80 AXaIU / ml. The AB cord blood plasma and mesenchymal stem cells come from the same provider. By means of the mesenchymal stem cell preservation liquid provided by the invention, the activity maintaining time of the mesenchymal stem cells can be prolonged (above 90% of activity is kept at 4-15 DEG C for 72 h); after the mesenchymal stem cells are preserved in the preservation liquid for 72h, the cells are normal in form, the proliferation and amplification capability of the cells cannot be influenced, and the phenotypic characteristics of the mesenchymal stem cells cannot be influenced; the mesenchymal stem cell preservation liquid disclosed by the invention is safe and reliable for clinical application; and, because the AB cord blood plasma and the cord mesenchymal stem cells come from the same placenta and belong to auto-plasma, the possibility of exogenous virus pollution is reduced.

The invention provides a stabilizing and buffering liquid of low molecular weight sugars used for lyophilization preservation of platelet, which is water solution with 35-45mM thickness by adding thelow molecular weight sugars on the basis of basic buffering liquid composing prescriptions (GS), wherein the basic buffering liquid composing prescriptions (GS) comprises 140mMNaCL, 5Mmkcl,12mM sodiumcitrate, 10mM glucose, 12.5mM sucrose and 1mug / mLPGE1. The low molecular weight sugars are trehalose. After the platelet is stabilized and protected by the stabilizing and buffering liquid of low molecular weight sugars of the invention, the platelet is freeze-dried with rehydration. The recovery ratio of a cell is above 70% and accumulating activity evoked by ADP can achieve 50% of fresh platelet. The platelet freeze-dried with rehydration has normal shape and complete structure, thereby a new solution is provided for freeze-drying conservation of the platelet.

The invention relates to a post weld heat treatment (PWHT) method for flash welding joints of U71MnH rails, and belongs to the technical field of rail welding. The invention provides the PWHT method for the flash welding joints of the U71MnH rails in order to solve the problem that when existing rail welding joints are cooled during heat treatment, the hardness of the joints is affected by martensite and other harmful structures which are formed because local cooling rate is higher than critical transition temperature. The PWHT method comprises the steps of heating U71MnH rail welding joint welding seam areas, which are cooled to be below 300 DEG C after welding, to 919-940 DEG C and then stopping heating, performing air jet cooling, stopping air jet cooling after the temperature of treadsurfaces of the joints of the rails drops to 410-435 DEG C, and performing natural cooling to room temperature. While effectively controlling the hardness of the welding joints of the U71MnH rails, the PWHT method ensures the normality of the structures of the joints.

The invention discloses a novel application of nitrate on an aspect of preventing radiation-induced salivary gland damage, and belongs to the fields of medicine and health. Upon clinical researches and animal experiments, it finally verifies that the nitrate, through oral administration, can be used for effectively preventing the radiation-induced salivary gland damage and for improving the symptom of xerostomia in patients with head and neck tumors due to radiotherapy. The nitrate disclosed by the invention can be used for preparing medicine which is effective on preventing the radiation-induced salivary gland damage; therefore, a novel way is provided for the clinical treatment of radiation-induced xerostomia.

The invention discloses a PyTPS (porphyra yezoensis ueda trehalose-6-phosphate synthase) gene which is cloned to construct a plant expression vector of the gene, and the plant expression vector is transferred into the agrobactrium tumefaciens strain, so that an engineering strain is obtained. A rice variety is transformed by utilizing agrobacterium-mediated transformation, so that genetically modified rice is obtained; after growing and acclimatization, a T0 individual plant is obtained, and a T1 seed is collected; a seedling germinated from the T1-generation genetically modified seed receives PCR (polymerase chain reaction) detection and a salt tolerance assay, so that a T1-generation genetically modified salt-resistant strain is obtained, a T2 generation receives a kanamycin resistance assay, PCR detection and Southern hybridization, and results indicate that the PyTPS gene is already integrated into the genome of the genetically modified strain. The PyTPS gene in the invention has a TPS domain as well as a TPP (thiamine pyrophosphate) domain; the PyTPS gene source is safe; the development of the plant morphology of the PyTPS genetically modified rice is normal; the PyTPS gene can be stably inherited in genetically modified plants; and moreover, the transformation system is highly effective.

The invention relates to a post-welding heat treatment method of U71MnH rail welded joints, and belongs to the technical field of rail welding. The post-welding heat treatment method of the U71MnH rail welded joints aims at solving the problem that the hardness of the joints is affected by harmful tissues such as martensite generated due to the local cooling speed being higher than the critical transition temperature when existing rail welded joints are cooled in the heat treatment process. The post-welding heat treatment method of the U71MnH rail welded joints comprises the following steps ofheating welding seam areas of the U71MnH rail welded joints cooled to below 300 DEG C after being welded to 865-925 DEG C, stopping heating, adopting air spraying for cooling, stopping air spraying for cooling after the temperature of the tread surfaces of the rail joints drops to 458-475 DEG C, and naturally cooling to room temperature. According to the post-welding heat treatment method of theU71MnH rail welded joints, the hardness of the U71MnH rail welded joints is effectively controlled, and meanwhile, normal tissues of the joints is ensured.

A full-function ring-cutting electric hair follicle transplanting pen comprises a pen head and a tubular pen tip.A motor is mounted in the pen head, the pen tip is driven by the motor to rotate, the tubular pen tip is circumferentially provided with 2-4 rectangular windows uniformly, a window bottom edge of each rectangular window inclines from the outside above to the inside below, an annular sliding sleeve sleeves outside the tubular pen tip in a manner of being capable of sliding up and down, 2-4 claw-shaped blades are integrally connected below the annular sliding sleeve, the bottom edge of each blade forms a sharp cutting edge, each blade correspondingly extends into the corresponding rectangular window, and a vertical pushing mechanism pushing the annular sliding sleeve to move vertically and a vertical positioning mechanism for locating a vertical position of the annular sliding sleeve are further arranged.By using the full-function ring-cutting electric hair follicle transplanting pen, all operation steps from electric hair extracting from a supplying area to electric hair planting from a receiving area can be completed smoothly, and the number of hair of a patient can be multiplied.

The invention discloses a method for obtaining dwarfed early gold sweet orange regeneration plant through agrobacterium rhizogenes. According to the characteristic of dwarfed plant type of plant transformed and regenerated through the agrobacterium rhizogenes, polyembryony breed of early gold sweet orange is transformed through agrobacterium rhizogenes NSU440, hairy roots are induced, then complete plant is obtained through a way of germination of hairy roots and radication of regeneration buds, and finally a dwarfed breed suitable for labor-saving cultivation is cultivated, so that the fruit garden labor efficiency is improved, and the labor expenditure is reduced. Transformation receptor material adopts early gold sweet orange aseptic seedling epicotyl, and as sweet orange belongs to a polyembryony breed, regeneration plant can keep parental excellent quality.

The invention relates to a traditional Chinese medicine decoction for treating severe acute pancreatitis and belongs to the technical field of traditional Chinese herbal medicine preparations for treating pancreatitis. The traditional Chinese medicine decoction is decocted by nineteen traditional Chinese herbal medicines such as gypsum, rheum officinale, mirabilite and rhizoma anemarrhenae. The traditional Chinese medicine decoction is used for treating the severe acute pancreatitis with the comprehensive synergistic effect of the nineteen traditional Chinese herbal medicines according to the theory of traditional Chinese medical recipe based on the traditional Chinese medicine principle of research and treatment on pathogenesis of severe acute pancreatitis. The traditional Chinese medicine decoction has the functions of clearing heat and removing toxicity, invigorating pulse-beat and defecating feces excretion and regulating qi to remove blood stasis, is few in side effects on the human body, simple in treatment method, low in cost, convenient to take and free of toxic and side effects, and is safe and effective. The whole recipe of the traditional Chinese medicine decoction has the functions of clearing heat and removing toxicity, invigorating pulse-beat and defecating feces excretion, regulating qi to remove blood stasis and treating both symptoms and root causes; and the effective rate of treatment on the severe acute pancreatitis reaches over 87.5%.

The invention belongs to the field of biomedicine and discloses a mesenchymal stem cell preservation solution and its preparation method and application. The mesenchymal stem cell preservation solution contains human AB type umbilical cord blood plasma and 70- 80AXaIU / ml calcium low molecular weight heparin; the human AB type umbilical cord blood plasma and mesenchymal stem cells come from the same provider. The mesenchymal stem cell preservation solution of the present invention can prolong the activity maintenance time of mesenchymal stem cells (maintain more than 90% of the activity for 72 hours at 4-15°C), and the mesenchymal stem cells are stored in the preservation solution of the present invention for 72 hours. , the cell morphology is normal, does not affect its proliferation and expansion ability, and does not affect the phenotypic characteristics of mesenchymal stem cells. The mesenchymal stem cell preservation solution of the present invention is safe and reliable in clinical application, and the AB-type umbilical cord blood plasma and umbilical cord mesenchymal stem cells are derived from the same placenta and belong to autologous plasma, which reduces the possibility of exogenous virus contamination.