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Glass freezing preserving method for fish embryo

A vitrification and preservation method technology, applied in fish farming, climate change adaptation, application, etc., can solve the problems of failure, failure to establish vitrification preservation technology, and failure to find out, so as to achieve normal shape and cool down The effect of high rate and reducing the formation of ice crystals

Inactive Publication Date: 2004-12-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

Therefore, although some scholars have conducted many studies on the vitrification technology of freshwater fish embryos, they have not been successful so far, and no feasible vitrification cryopreservation technology has been established.
However, no literature or patent reports have been found regarding the cryopreservation of seawater fish embryos, especially the vitrification cryopreservation technology of flounder and perch embryos

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0019] The technical route of vitrification and cryopreservation of fish embryos is: collection of embryos → determination of embryo quality and development stage → screening of vitrification base fluid and antifreeze → preparation of vitrification fluid → balance of embryos in vitrification fluid before freezing → Transfer of embryos to straw, sealing of straw, equilibration of straw containing embryos → cryopreservation in liquid nitrogen → thawing of straw → elution and equilibration of embryos → culture of embryos.

[0020] The successful experiment of vitrification preservation with flounder embryos is described as follows:

[0021] (1) Embryo collection: Carry out productive seedling raising of flounder according to conventional techniques, collect fertilized eggs of flounder naturally spawned under artificial conditions and culture them at 14-16°C, observe embryonic development stages with a microscope, and select the stage of development to the appearance of sarcomere, ...

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Abstract

A vitrifying freeze storage method for the embryos of fish, especially the bastard halibut, is disclosed, which features that the formula of vitrifying liquid suitable for the embryo of seawater fish is screened out, the embryo development stage suitable for vitrifying it is determined, a particular eluting solution is disclosed, and the step balance and the fast cooling and thawing techniques are used. Its advantages are high safety, easy operation and low cost.

Description

Technical field: [0001] The invention belongs to the cryopreservation technology of animal embryos in cryobiology, and relates to a vitrification freezing method for long-term preservation of fish embryos in liquid nitrogen (-196 DEG C). Background technique: [0002] Germplasm resources are an important material basis for aquaculture production, breeding of fine varieties and sustainable development of aquaculture industry. my country is a country rich in aquatic organism germplasm resources. The rich and diverse aquatic organism germplasm resources and genetic diversity have played a very important role in the rapid development of my country's aquaculture industry. However, due to overfishing, disorderly utilization and artificial release of fishery resources, some fish resources have declined and become endangered, such as Yangtze shad and Songjiang perch. If protection measures are not taken in time, it will be difficult to find many genetic resources of original and im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈松林田永胜
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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