The invention relates to a modified zebra fish
drug metabolism modeling method, which specifically comprises the steps of (1) enriching metabolites by virtue of a zebra fish
metabolism model; (2) separating and analyzing the enriched metabolic components of the zebra fish in the last step by using modern
chromatography or the hyphenated technique thereof, capturing or knocking out target components or metabolites; (3) collecting zebra fish germ cells; (4) putting zebra fish embryos in three days after fertilization in a culture plate which holds an
embryo culture medium and administrating a
drug; (5)
dyeing the bones of the zebra fish; and (6) detecting and analyzing the dyed bones of the zebra fish. According to the invention, an adolescent zebra fish
osteoporosis model is applicable to in-vivo effective evaluation of anti-
osteoporosis activity of Chinese herbal medicinal ingredients such as trace ingredients; an adult zebra fish
metabolism model is simple in metabolites and efficient in enrichment; the modern
chromatography or the hyphenated technique thereof provide powerful guarantee for the separation and analysis of the complex Chinese herbal medicinal
system and the metabolic components. The model obtained by the method has the outstanding
advantage that the separation and analysis of Chinese herbal medicinal original-form ingredients and the metabolic components are integrated with the in-vivo anti-
osteoporosis activity evaluation, and simple and efficient.