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Application of zebra fish to testing safety of safe substance and method for applying zebra fish to test safety of safe substance

A technology of zebrafish and zebrafish embryos, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of long test cycle, inability to see food additives, human hazards, etc., and achieve the effect of shortening the detection cycle

Inactive Publication Date: 2013-01-30
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Claims
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Problems solved by technology

[0004] In particular, food additives, in order to meet certain indicators of food, substances that cannot be clearly determined for the safety of organisms and human bodies are added. If these substances are tested in organisms, the test period will be relatively long. The tolerance level of safe substances, it is impossible to see whether a certain food additive has invisible harm to the human body in the short term

Method used

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  • Application of zebra fish to testing safety of safe substance and method for applying zebra fish to test safety of safe substance
  • Application of zebra fish to testing safety of safe substance and method for applying zebra fish to test safety of safe substance
  • Application of zebra fish to testing safety of safe substance and method for applying zebra fish to test safety of safe substance

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Embodiment 1

[0022] The screening of embodiment 1 optimal embryo culture medium

[0023] 1.1 The preparation of different formulas of embryo culture medium and the effect of culturing zebrafish

[0024] A Prepare embryo culture solution containing the following concentrations of inorganic salts with ordinary water, adjust the pH to 7.2, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 1:

[0025] Table 1 Effects of embryo culture medium prepared with ordinary water on zebrafish embryos

[0026]

[0027] B Use weakly alkaline water with a pH of 7.2 prepared by electrolysis of pure water to prepare an embryo culture solution containing the following concentrations of inorganic salts, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 2:

[0028] Table 2 Effects of embryo culture medium prepared with weakly ...

Embodiment 2

[0032] Embodiment 2 Zebrafish tests the toxicity of safe substances

[0033] Add the safety substance to be tested with the embryo culture solution of the above-mentioned numbers A1 and B1 respectively, the content (mass content) of the safe substance to be tested is prepared in steps, and the solution is subjected to zebrafish toxicity determination, wherein each treatment is repeated 5 times, The average value was taken, and the controls were embryo culture solutions A1 and B1 without safety substances added. The determination is mainly carried out through the following steps:

[0034] 1) The cultivation of zebrafish:

[0035] The male and female common zebrafish of the sexually mature AB line were separated into separate tanks, and were raised in a circulating water culture system, with 14 hours of lighting and 10 hours of darkness alternately, the water temperature was kept at 28°C, and brine shrimp artificially incubated for 24 hours were regularly fed;

[0036] 2) Embr...

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Abstract

The invention relates to an application of zebra fish to testing safety of a safe substance and a method for applying zebra fish to test safety of the safe substance. The method is characterized by comprising the following steps of: preparing an embryo culture solution, then adding the safe substance to be tested to the embryo culture solution, using the embryo culture solution to which the safe substance to be tested is added to culture zebra fish embryos and then observing and recording the developmental aberration rates and mortality of the zebra fish embryos in 4-72 hours to judge the toxicity of the safe substance to be tested toward development of the zebra fish embryos, wherein the addition of the safe substance to be tested ensures that the concentration of the substance to be tested in the culture solution is distributed in echelon; the embryo culture solution is directly prepared from alkalescent water with pH value being 6.7-8.5; and the embryo culture solution contains 5mM of NaCl, 0.17mM of KCl, 0.43mM of CaCl2 and 0.33mM of MgSO4. The method has the following beneficial effect that the embryo culture solution prepared from the alkalescent water ensures that the zebra fish embryos can quickly test the comprehensive toxicity of the safe substance to be tested, thus shortening the detection period.

Description

technical field [0001] The invention relates to the field of toxicity detection, in particular to a use and method of zebrafish for testing the safety of safe substances. Background technique [0002] With the reduction of natural production methods and the increasing proportion of industrialization, some chemical substances will be introduced consciously or unconsciously in the process of production, storage, transportation and processing of food raw materials, which will bring more and more insecurity to food safety. factor. [0003] The use of chemical substances in food has a long history. It is accompanied by chemical substances in the production, processing, storage, transportation and consumption of modern food. It is the wide use of these chemical substances in food that makes the food industry a sunrise. industry. However, the use of chemical substances also has a non-negligible impact on food safety, including the impact of drug residues, heavy metals, natural to...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 陈峰
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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