39 results about "Optical projection tomography" patented technology

The invention relates to a method for the examination by tomography of a sample (9). According to said method, a sample (9) is illuminated with an illumination light beam (3), and a transmission light beam (10), which contains the light of the illumination light beam (3) transmitted through the sample (9), is detected by means of a transmission detector (13). The invention further relates to a device for the examination of a sample (9) by tomography. The illumination light beam (3) and the transmission light beam (10) pass through the same lens (7) with opposite propagation directions.

The invention discloses a method and device for marking the center of rotation based on an optical projection tomography sinogram. The method includes: the projection rotation center of the marker and the sample is the same, the first section sinogram and the second section sinogram are obtained, and the calculation The balance position of the first section sinogram is calculated by calculating the distance from the balance position to the upper edge and the lower edge of the first section sinogram to obtain the target height value, and the second section sinogram is longitudinally cut or longitudinally repaired, so The height values ​​of the longitudinal cropping and the longitudinal repairing are the target height values, and the back projection reconstruction is performed on the sinogram of the second section after the longitudinal cutting or longitudinal repairing to obtain the target reconstructed cross-sectional view of the sample. The invention quickly calculates the projection rotation center of the sample by introducing markers as reference objects without being affected by the complexity of the sample structure, realizes the correction of the projection rotation center, reduces the artifacts of the reconstruction section, and improves the imaging quality.

The invention discloses an optical projection tomography method based on a merged spiral scanning mode. The optical projection tomography method comprises the following steps of: merging projection drawings on the same height, at the same angle and at different detector positions into a projection drawing which can cover all samples in the horizontal direction, and merging a plurality of local spiral projection data into a whole spiral projection data; calculating the axial visual field of the whole three-dimensional reconstruction body according to the axial position and scanning angle of each merged projection drawing; dividing the three-dimensional reconstruction body into a plurality of pieces of axial tomography to be reconstructed, performing data rearrangement on a projection line corresponding to each axial tomography to be reconstructed to obtain a sinusoidal chart corresponding to the axial tomography to be reconstructed; and reconstructing the sinusoidal chart into a tomography image by utilizing a circular track filtering back projection method, and sequentially superposing various tomography images to obtain the three-dimensional reconstruction body. According to the method, the visual filed of the optical projection tomography can be effectively widened, and the capacity of imaging a large-size object in an imaging system is improved.

The invention discloses an emission type optical projection tomography (OPT) attenuation and scattering correction method. An OPT model under the condition of weak scattering and an OPT fluorescence reconstruction algorithm are combined. OPT fluorescence signal attenuation correction is conducted using a normalized Born ratio method; the scattering component in measured data is simulated using the Monte Carlo technology, and the scattering component is removed from detection data; and through an iterative framework effectively combining attenuation correction and scattering component estimation, quantitative 3D reconstruction of OPT fluorescent dye concentration is realized. The influence of both absorption and scattering is considered comprehensively in quantitative reconstruction of fluorescent dye concentration, the scattering component in signals is estimated using the Monte Carlo technology and removed, and quantitative reconstruction of fluorescence dye concentration is realized.