57 results about "Subcellular structure" patented technology

The invention belongs to the technical field of microimaging and provides a method, an optical module and a system for extended field depth three-dimensional nanoscale-resolution imaging. The method comprises the following steps of 1, building the optical module having a double-helix point spread function and defocusing grating multistage imaging properties, 2, carrying out imaging of a molecule needing to be detected by the optical module to obtain a double-helix image, 3, determining a horizontal position of the molecule needing to be detected according to positions of middle points of double-helix sidelobes of the double-helix image, and 4, determining an axial position of the molecule needing to be detected according to a rotation angle of a connection line of centers of the double-helix sidelobes of the double-helix image, and the positions of the middle points of the double-helix sidelobes. Through combination of double effects of the double-helix point spread function and the defocusing grating multistage imaging, the method extends a field depth and improves a resolution ratio. The method can be used for dynamic range imaging of subcellular fractions having any depth values in a whole cell, can produce dynamic function images of multiple moving molecules, and has an important meaning for high-level understanding of a rule and a relationship between a subcellular structure and a cell function change.

Methods and systems for tissue classification of a tissue sample are provided. The methods and systems transform the tissue image data to a biomarker enhanced tissue network (BETN) such that an individual cell or a sub-cellular structure in the tissue image data corresponds to a node in the BETN, define a feature vector based on a feature set representative of a tissue type of interest, cluster nodes of the BETN based on a similarity criterion of one or features of the feature vector, and classify the nodes in the tissue image data based on the grouping of the nodes of the BETN.

Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, include treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by application of an initiation energy to a subject thus producing an effect on or change to the target structure directly or via a modulation agent. The methods may further be performed by application of an initiation energy to a subject in situ to activate a pharmaceutical agent directly or via an energy modulation agent, optionally in the presence of one or more plasmonics active agents, thus producing an effect on or change to the target structure. The invention provides kits containing products or compositions formulated or configured and systems for use in practicing these methods.

The present invention relates to a method based on atomic force microscopy and the use thereof on biological surfaces. A method is provided to detect the Local Deviational Volume (LDV) of defined subcellular structures irrespective of a biochemical characterization.

A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, including treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by application of an initiation energy to a subject thus producing an effect on or change to the target structure directly or via a modulation agent. The methods may further be performed by application of an initiation energy to a subject in situ to activate a pharmaceutical agent directly or via an energy modulation agent, optionally in the presence of one or more plasmonics active agents, thus producing an effect on or change to the target structure. Kits containing products or compositions formulated or configured and systems for use in practicing these methods.