46 results about "Immunofluorescent labeling" patented technology

An immune-fluorescence labeling method of micro-pipe frame at pollen tube on gymnosperm includes fixing pollen tube of gymnosperm on fixing liquid for 40-60min, washing pollen tube, placing pollen tube in enzyme solution for carrying out enzymolysis of 30-40 min under temperature of 20-30deg.c, washing pollen tube, setting pollen tube in Triton X-100 to penetrate there for 1-3h, washing pollen tube, adding anti-beta-tubulin single clone antibody to carry out hatching for 1-3h, washing pollen tube, adding fluorescence label to carry out shielded hatching for 1-3h for making micro-pipe frame be immune-fluorescence labeled.

The invention discloses an upconversion super-resolution imaging nanoprobe as well as a preparation method and application thereof. The upconversion super-resolution imaging nanoprobe has a core@shellstructure. The preparation method comprises the following steps: preparing rare-earth upconversion nano-particles with controllable particle size and excellent dispersion property by adopting a solvothermal method; performing surface modification by polyacrylic acid; and finally performing protein modification. The upconversion super-resolution imaging nanoprobe is applied to immunofluorescent labeling of a subcellular structure of cells, so that fluorescent super-resolution imaging of the subcellular structure is realized. Compared with the traditional fluorescein-based super-resolution optical imaging method, the method disclosed by the invention is a fluorescence zero-bleaching and zero-flicker super-resolution microscopic imaging method based on the upconversion super-resolution imaging nanoprobe, and biological samples can be observed for a long time.

The invention relates to an immunofluorescent kit for detecting different loci of an HER2 antigen and an application of the immunofluorescent kit. A detection principle of the method disclosed by theinvention is as follows: firstly, enriching non-humoral rare nucleated cells, and then, detecting the expression of target proteins in the enriched cells in cells by using an immunofluorescene detection method according to an antigen-antibody reaction principle. According to the kit, a common antigen CD45 of a target cell and a leukocyte is fluorescently labeled, and the cells with positive targetproteins and negative CD45 are screened, so that the non-humoral rare nucleated cells with positive specific proteins in the blood are interpreted and counted.

The invention discloses a multi-color immunofluorescence labelling method and an imaging method, and relates to the technical field of immunofluorescence. The multi-color immunofluorescence labellingmethod comprises a plurality of fluorescence labelling steps and crosslinking fixing steps of incubating a sample with a crosslinking fixative after each fluorescent labelling step. The crosslinking fixative comprises acetone, paraformaldehyde and PBS buffer. According to the multi-color immunofluorescence labelling method, various kinds of different fluorescent dyes can be labelled on various kinds of antigen markers in the sample. The labelled sample can be used for confocal microscopy imaging to display various kinds of different colors, and the colors are clear, bright and uncolored. The method provides a basis for scientific research workers to simultaneously observe the properties, positioning and content of various kinds of antigens.

The invention provides a method able to realize separation, amplification and purification of embryonic cranial neural stem cells rapidly and simply. The invention uses the thermal conversion polymer N-isopropylacrylamide and polyethylene glycol to conduct three-dimensional culture on neural cells, and makes use of the characteristic that neural stem cells can proliferate in a gel environment and form neurospheres to achieve the purposes of separation and purification of the neural stem cells. The formed neurospheres are subjected to immunofluorescent labeling determination, which shows that about 93% of the cells are Nestin positive, so that the method is simple and effective for separation and purification of neural stem cells. The embryonic neural stem cells separated and purified by the method can differentiate in vitro to form neurons, oligodendrocytes and Glias.

The invention relates to a method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water. The method comprises the following steps: adding an inorganic metal salt coagulant into a bulk water sample, stirring, coagulating, precipitating, collecting precipitate alumen ustum in a small-volume container, adding acidic solution into the small-volume container to dissolve the alumen ustum, centrifuging, removing supernate and thus, enriching the Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water. In the invention, the problems of low Cryptosporidium parvum oocyst and Giardia Lamblia sporocyst enrichment rate, instability, high operation requirement and expensive filtering equipment and consumable items of the conventional method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts. The method does not need expensive apparatus and equipment and medicines and greatly reduce capital investment. The method is simple in operation, easy to master, time-saving and labor-saving, reduces cost of enrichment of Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water, improves enrichment efficiency and is a novel method which clears the preliminary work for subsequent steps of immunomagnetic separation, immunofluorescence label microscope counting, polymerase chain reaction (PCR) qualitative detection, nano probe gene chip detection and Real-time PCR quantitative detection and the like.

The invention discloses a method for quantitatively analyzing nicotine-induced cell DNA damage based on the high content technology. The method includes the steps of firstly, culturing cells; infecting the cells; thirdly, performing immunofluorescent labeling of gamma H2AX; fourthly, performing high-content-technology quantitative analysis. The method has the advantages that a high-content imaging system is used to perform automatic imaging, DNA double-strand break marker gamma H2AX protein generated by nicotine induction is quantitatively analyzed, direct and fast cell detection is achieved, and sample processing convenience is achieved; the high-resolution imaging allows the distribution of the gamma H2AX in each cell nucleus to be able to be observed directly and image data to be convenient to store and reanalyze, the nicotine-induced gamma H2AX in each cell nucleus can be quantitatively analyzed, and the detection result is sensitive and accurate.

The invention discloses a rhodamine dye containing a water-soluble substituent and a functional derivative (a zinc ion probe and an antibody labeled dye) of the rhodamine dye. The rhodamine dye has a structure as shown in a general formula I. After the N-site of a parent nucleus of the dye is connected with different water-soluble substituents, the biocompatibility of the parent nucleus of the dye is greatly improved; and specifically, non-specific staining in cells is remarkably reduced, cell apoptosis signals do not appear any more, phototoxicity is remarkably reduced (the zinc ion probe), labeling efficiency is improved, observation time is prolonged (the antibody labeled dye) and the like. The fluorescent dye disclosed by the invention can be applied to the fields of observing insulin release of in-vitro live pancreas islets, immunofluorescence labeling and the like.

The invention belongs to the field of biological imaging of nanomaterials, and discloses a nano fluorescent probe solution for liver cell labeling below freezing point and its application. The nano fluorescent probe solution includes nano fluorescent probes dispersed therein as a dispersoid; The needle includes nano fluorescent particles and targeting molecules coupled on the surface of the nano fluorescent particles, the targeting molecules can be used to target specific cells in the liver; and the nano fluorescent probe solution also includes antifreeze substances, the concentration of the antifreeze substances satisfies the nano Fluorescent probe solutions remain liquid at temperatures below freezing. In the present invention, by improving the detailed structure and composition of the nano fluorescent probe solution, the specific liver cells of the pre-selected cell type can be used as the target cells, and based on the nano fluorescent probe, the liver cells can be immunofluorescently labeled below freezing point , has the characteristics of rapidity, simplicity and high efficiency, and is especially suitable for use as a fluorescent marker in liver micro-optical section tomography.