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Prognostic evaluation method for hepatocellular carcinoma through Telocytes

A technology of hepatocellular carcinoma and evaluation method, which is applied in the field of prognosis evaluation of hepatocellular carcinoma, and can solve the problems of high cost and long observation time.

Inactive Publication Date: 2022-02-11
许莹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In tumor pathological tissues, counting the number of TCs needs to be stained by immunohistochemistry or immunofluorescence indicators, and cell morphology is identified by transmission electron microscopy. This process requires high cost and long observation time

Method used

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  • Prognostic evaluation method for hepatocellular carcinoma through Telocytes
  • Prognostic evaluation method for hepatocellular carcinoma through Telocytes
  • Prognostic evaluation method for hepatocellular carcinoma through Telocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for evaluating the prognosis of hepatocellular carcinoma by Telocytes, comprising the following steps:

[0029] S1: Take postoperative tumor samples from patients with hepatocellular carcinoma, use high-speed crushing, high-speed grinding, high-speed stirring and supramolecular penetration technology in liquid solvents at room temperature to rapidly treat cancer cells in adjacent tissues within 1-2cm from the tumor The adjacent tissue was crushed and ground to fine particles, and the tissue cells with complete morphology were extracted. The tissue cells adjacent to the cancer cells were fixed in 4% paraformaldehyde buffer, embedded in paraffin in JB-P5 paraffin embedding machine and archived. , and then prepared 3 μm thick tissue sections with RM2016 pathological slicer. The slices were dehydrated in triplicate with xylene (15 min each time), dehydrated with pure ethanol twice (5 min each time), the ethanol gradient was 85% and 75% (5Min), and the slices were s...

Embodiment 2

[0035] S1: Take postoperative tumor samples from patients with hepatocellular carcinoma, use high-speed crushing, high-speed grinding, high-speed stirring and supramolecular penetration technology in liquid solvents at room temperature to rapidly treat cancer cells in adjacent tissues within 1-2cm from the tumor The adjacent tissue was crushed and ground to fine particles, and the tissue cells with complete morphology were extracted. The tissue cells adjacent to the cancer cells were fixed in 4% paraformaldehyde buffer, embedded in paraffin in JB-P5 paraffin embedding machine and archived. , and then prepared 3 μm thick tissue sections with RM2016 pathological slicer. The slices were dehydrated in triplicate with xylene (15 min each time), dehydrated with pure ethanol twice (5 min each time), the ethanol gradient was 85% and 75% (5Min), and the slices were stored in sodium citrate antigen extraction solution (ServiceBio: G1202, pH Incubate for 3 times in PBS (Servicebio: G0002...

Embodiment 3

[0041] S1: Take postoperative tumor samples from patients with hepatocellular carcinoma, use high-speed crushing, high-speed grinding, high-speed stirring and supramolecular penetration technology in liquid solvents at room temperature to rapidly treat cancer cells in adjacent tissues within 1-2cm from the tumor The adjacent tissue was crushed and ground to fine particles, and the tissue cells with complete morphology were extracted. The tissue cells adjacent to the cancer cells were fixed in 4% paraformaldehyde buffer, embedded in paraffin in JB-P5 paraffin embedding machine and archived. , and then prepared 3 μm thick tissue sections with RM2016 pathological slicer. The slices were dehydrated in triplicate with xylene (15 min each time), dehydrated with pure ethanol twice (5 min each time), the ethanol gradient was 85% and 75% (5Min), and the slices were stored in sodium citrate antigen extraction solution (ServiceBio: G1202, pH Incubate for 3 times in PBS (Servicebio: G0002...

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Abstract

The invention relates to the technical field of clinical medicine, in particular to a prognostic evaluation method for hepatocellular carcinoma through Telocytes, which comprises the following steps: taking a postoperative tumor specimen of a hepatocellular carcinoma patient, preparing a frozen section or a paraffin section from a cancer cell para-tissue within a range of 1-2cm from the tumor, carrying out immunofluorescent staining by using CD34 (highly glycosylated I-type transmembrane glycoprotein) / CD117 (III-type transmembrane kinase receptor protein) / PDGFR (platelet-derived growth factor receptor) / vimentin (waveform fibrin) indexes, taking CD34 + / CD117 + / PDGFR + / vimentin-cells as TCs cells, putting para-carcinoma tissues into a general stationary liquid for an electron microscope, performing slicing, and observing the morphology of the TCs cells under a transmission electron microscope. According to the prognostic evaluation method for the hepatocellular carcinoma through the Telocytes, multiple groups of double immunofluorescence markers are adopted, and the cell morphology and the telomere number are observed, so that accurate indexes for prognostic evaluation of patients can be provided for clinicians, and the problem of probability of prognostic evaluation indexes of the patients with the hepatocellular carcinoma is solved.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a method for evaluating the prognosis of hepatocellular carcinoma through Telocytes. Background technique [0002] Telocytes (TCs), as a new type of mesenchymal-derived cells, widely exist in human organs and tissues, and participate in a variety of physiological and pathological processes, such as tissue repair, cardiac ischemia-reperfusion injury, atherosclerosis, and tumorigenesis Wait. In human liver tissue, TCs are positive for CD34, PDGFR-α and CD117, and negative for vimentin (Vimentin) and CD28. The processes involved in the occurrence and development of diseases are called "Telocytopathies". [0003] TCs can be involved in the occurrence and development of inflammatory colonic polyps and PDGFR-A mutant colonic diseases as tumor-promoting factors; TCs can also transmit information with surrounding cells to participate in various lung disease processes. In prev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/04G01N21/64G01N1/28
CPCG01N23/04G01N21/6458G01N1/28G01N2223/03G01N2223/102
Inventor 许莹栾光超陈云松褚益福李腾秦广洋
Owner 许莹
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