Method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water
A technology of Giardia and Cryptosporidium is applied in the field of coagulation and enrichment of Cryptosporidium oocysts and Giardia cysts in water, which can solve the problems of low enrichment rate of the two worms, high operation requirements and high price. , to achieve the effect of reducing capital investment, improving enrichment rate and simple operation
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Embodiment 1
[0018] Using tap water as a water sample, polyaluminum chloride (PAC) and polyaluminum ferric chloride (PAFC) were used as coagulants to coagulate Cryptosporidium oocysts and Giardia cysts added to the water sample Enrichment. The sample volume of tap water is 1L, and Cryptosporidium oocysts and Giardia cysts are added respectively, so that the water contains about 1000 Cryptosporidium oocysts and Giardia cysts, and the pH value of the water sample is 9.0; The dosage is 30mg / L, the dosage of PAFC is 20mg / L, the stirring condition is rapid stirring at 180r / min for 2min, then slow stirring at 40r / min for 15min, and standing for 30min after the reaction.
[0019] Then discard the supernatant, collect the precipitated alum flowers, add 1 mL of 1.0 mol / L hydrochloric acid to the collected alum flowers, and stir to dissolve the alum flowers. The solution was centrifuged at 10,000 rpm for 10 minutes, then the supernatant was sucked out, and the sediment at the bottom of the centrifu...
Embodiment 2
[0024] Taking the water of Xinglinwan Reservoir in Xiamen City as a sample, using aluminum sulfate, ferric sulfate, ferric chloride, PAC, and PAFC as coagulants, the surrogate indicator (fluorescent Microspheres, divided into Φ6.0 μm for simulating Cryptosporidium oocysts and Φ10.0 μm for simulating Giardia lamblia cysts, were purchased from Polysciences, USA) for coagulation enrichment. The enrichment method is the same as in Example 1.
[0025] Table 2 shows the enrichment rate of Cryptosporidium microspheres and Giardia microspheres in surface water according to the present invention in Example 2.
[0026] Table 2 Enrichment rate of Cryptosporidium microspheres and Giardia microspheres added in surface water by different coagulants
[0027]
[0028] Visible by above embodiment, the present invention is to the enrichment rate of Cryptosporidium oocyst in tap water 73.8-92.9%, the enrichment rate of Giardia cyst is 73.8-78.6%, and Cryptosporidium microsphere and The high...
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