648results about How to "High detection throughput" patented technology

The invention provides a chemiluminescence immunoassay device which comprises a sample supply mechanism, a chemical reaction incubation mechanism, a sample adding mechanism and a luminescence detection mechanism, wherein the sample supply mechanism comprises a plurality of sample tubes, a sample disc, a plurality of reagent tubes and a reagent disc, wherein the sample disc and the reagent disc are respectively in a circular ring shape and can rotate. The chemical reaction incubation mechanism is set to be at constant temperature, and comprises a rotary table, a plurality of reaction discs, a plurality of reaction cups and a driving mechanism, wherein the rotary table is in a circular shape; the plurality of reaction discs are arranged on the rotary table at equal intervals along the circumferential direction of the rotary table; reaction cup containing cavities are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the plurality of reaction cups are respectively arranged along the circumferential directions of the reaction discs at equal intervals; the driving mechanism is connected with the rotary table and the plurality of reaction discs by power, so that the rotary table can rotate, and the reaction discs are driven to make revolution or respectively rotate. The sample adding mechanism comprises a liner movement unit, a sample needle, a reagent needle and a cleaning groove. The luminescence detection mechanism comprises a cleaning needle, a substrate needle, a light insulation cover and a detection head.

The patent of the invention discloses a multi-parameter oil detecting device and a manufacturing method thereof. The detecting device is used for distinguished detecting ferromagnetic metal particles,non-ferromagnetic metal particles, water drops and bubbles in oil. Based on a manufacturing method of a micro-fluidic chip, the dead center of a micro-channel is filled with a metal rod for the firsttime, and a capacitance detection mode is introduced; a ring runner is arranged in the micro-fluidic chip for the first time, an inner hole of a planar inductance coil is sufficiently used, metal particles are enabled to pass through a most sensitive detection area, and the detection flux of the chip is improved when the detection precision is ensured; and a vertical runner is adopted to reduce the adhesion possibility of pollutants in oil on the wall of the runner, and the deposition and blockage of the pollutants in the runner are effectively prevented. The chip can complete the distinguished detection of particulate pollutants in lubricating oil and hydraulic oil, a new method is provided for comprehensive rapid detection of machines and devices on oil, and fault diagnosis can be performed on the machines and the devices.

The invention discloses a genome simplification and next-generation sequencing-based deoxyribose nucleic acid (DNA) library preparation method and a kit in the field of biotechnologies. The method and the kit aim to overcome the defects of the conventional library preparation method and can be used for whole genome single nucleotide polymorphism (SNP) detection and genetic typing of species of which the reference genomes are not perfect and the genealogies of research groups are not clear, and which do not have haplotype maps. The DNA library preparation method and the kit are simple in operation process, the prepared library is high in sequencing quality, the segment distribution among individuals is low in variability, the research cost is low, and the DNA library preparation method and the kit have very wide application prospect in the aspect of realizing the high-throughout whole-genome SNP detection and genetic typing research.

The invention relates to a method for simultaneously detecting five kinds of steroid hormones in serum. The five kinds of steroid hormones are respectively testosterone, androstenedione, 11-deoxycortisol, cortisol and cortisone. The method for simultaneously detecting the five kinds of steroid hormones in the serum comprises the following steps: sample pretreatment: adding an acetonitrile solution of an internal standard substance in a serum sample for carrying out protein precipitation, then adding tert-butyl methyl ether for extracting, blow-drying supernatant, adding a combination solution, and taking the supernatant, thus obtaining a to-be-detected sample; enrichment, separation and detection: carrying out enrichment, separation and detection on the to-be-detected sample by adopting a two-dimensional liquid chromatography-tandem quadrupole mass spectrometer. According to the method for simultaneously detecting the five kinds of steroid hormones in the serum, disclosed by the invention, the method is adopted for simultaneously detecting five kinds of compounds of which the concentration ranges are inconsistent in the serum, and the time for simultaneously detecting the five kinds of the compounds is about 8.5 to 12.0 minutes; the method for simultaneously detecting the five kinds of steroid hormones in the serum is low in cost, high in flux, high in precision degree and strong in specificity.

The invention discloses a deafness susceptibility gene mutation detection kit as well as a preparation method and application thereof, and relates to gene mutation detection. The kit comprises a kit body, a cover, a partition, an HHL (Hereditary Hearing Loss) PCR (Polymerase Chain Reaction) mixed liquor bottle A, an HHL PCR mixed liquor bottle B, an HHL PCR mixed liquor bottle C, an HHL PCR mixed liquor bottle D, an HHL enzyme mixed liquor bottle, an HHL standard control bottle, and an HHL negative control bottle. The preparation method comprises the following steps: preparing an amplification reagent and a contrast reagent firstly, arranging the amplification reagent and the contrast reagent in the bottles and finally, obtaining the deafness susceptibility gene mutation detection kit. The kit can be applied to screening of hereditary deafness genes and identification of deafness causing genes, so as to prevent deafness.

The invention relates to a detection chip, a detection kit and a detection method for bioterrorism factors. Probe molecules and PCR amplification primers are designed according to the gene conservation regions of various bioterrorism factors, and multiple probe molecules are integrated into one detection method. On the chip, the detection throughput is high. At the same time, due to the application of gene chip detection, the detection sensitivity is high and the speed is fast; according to the analysis of the designed PCR amplification primer sequence, multiple PCR technology can be used to detect nucleic acid substances of various bioterrorism factors at one time. Amplification, thereby further speeding up the detection speed.