58 results about "Hpv genotypes" patented technology

Embodiments of the invention provide methods, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and / or late HPV-associated or HPV-specific proteins or antibodies. Detection of HPV DNAs, genomes, and / or oncoproteins by protein chips immunological assays can be used in early clinical screening for HPV infection and general diagnosis for cervical cancer and can be advantageous performed in a multiplexed test. Comparative detection of altered levels of HPV proteins and host proteins can performed in one or more assays. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer.

This invention relates to one polymer enzyme linkage reaction fluorescence test method to dialogue dangerous human body nipple shape virus and to isolate DNA sample HPV gene type to test one set of dangerous HPV infection from patient by the method, wherein, it belongs to life science and biological technique. This invention agent case comprises one fluorescence meter PCR technique as base of multi-layer polymer enzyme reaction composed of multiple HPV positive lead object, reaction lead object and fluorescence detector.

Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and / or late stage HPV-associated or HPV-specific cancers. The anti-HPV antibodies are used in performing immunological assays on clinical samples. Various immunological assays and kits for detecting HPV infection, cervical cancer, other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression are also provided.

The invention provides a method for detecting the HPV genotypes, with the gene to be detected being one or several types of the type 17 HPV, comprising the following steps: according to the variant sites of the selected HPV genotype universal primer sequence to be detected, an amplification primer aiming at each type is designed; the specific extension primer of each type is designed; (2) PCR amplification is conducted; (3) SAP enzyme treatment is conducted; (4) extension reaction is conducted, among the extending products and the extending primers, the difference of the molecular weight among the extending products of each type is not less than 9D; (5) resin is used to purify extension reaction products; and (6) mass spectrometry detection is conducted, and the type of HPV gene to be detected is determined. By using the method, the invention solves the problems of some of existing detection methods that the typing can not be realized, the multiple infections can not be detected, the accuracy is limited, the throughput is low, the cost is high, and the stability of reaction may be affected as the probe is RNA.

Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and DNA chips were produced by using the oligonucleotide probes. The synthesis of the oligonucleotide probes is based on clones of Ll and E6 / E7 genes of 35 types of HPV obtained from cervical cell specimens from 4,898 Korean adult women and tissue specimens from 68 cervical cancer cases in addition to information based on American and European cases. The DNA chips can analyze the 40 types of HPV found in cervical, diagnose complex infection by at least one type of HPV, and have excellent diagnostic sensitivity and specificity on HPV genetic type up to 100% and reproducibility. Also, the DAN chips are superior to conventional analytic method, and very economical, since they can analyze numerous specimens in shortest time. Accordingly, the DNA chips are useful for predicting cervical cancer and precancerous lesion.

The present invention discloses a primer, a probe, a DNA chip, and a test kit for diagnosis and genotyping of Human Papilloma Virus (HPV) as well as a method for testing HPV genotype. According to the present invention, it is possible to screen HPV genotypes with high sensitivity and specificity, and to diagnose infection by multiple HPV types which was not possible in other conventional HPV testing methods

The invention provides oligonucleotides, kits and methods for genotyping HPV.

The present invention relates to a DNA chip, or a DNA microarray, having a conglomeration of probes thereon, wherein the probes complementarily bind with 44 types of HPV nucleic acids, which are the main cause of cervical cancer and the most common cause of sexually transmitted diseases, a genotyping kit having same, and a method for genotyiping using same. The present invention enables recognition of all 44 types of HPV that invade the genitals, accurate diagnosis of multiple infections from more than one HPV types, high sensitivity and specificity for the HPV genotype diagnosis at near 100%, and quick testing of a plurality of specimens, and is very useful in prognosis of cervical cancer and precancerous lesions.

The invention discloses a multicolor high-risk HPV (human papilloma virus) fluorescence detection kit which comprises a warm-boot Taq enzyme system, universal primers, fluorescent probes, a negative control and a positive control, wherein the fluorescent probes are HPV-specific MGB probes. The kit is suitable for qualitatively detecting 18 high-risk HPV genotypes capable of causing cervical carcinoma in cervix exfoliated cell and genitourinary tract secretion samples. The kit uses the multiplex fluorescence PCR (polymerase chain reaction) technique to implement detection of 18 HPV genotypes in one tube amplification system. The kit can simultaneously detect 15 common high-risk HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 and 82) and can also detect 3 rare high-risk HPV genotypes (26, 53 and 73). The detection kit has the advantage of high detection flux and greatly lowers the detection cost.

The invention discloses a method for performing rapid gene typing on trace human papilloma virus (HPV), which has the characteristics of wide application range, high sensitivity, accuracy and high efficiency. Based on a polymerase chain reaction (PCR) detection method, nested amplification is performed on an HPV L1 conservative region for three times, electrophoresis detection and sequencing are performed on a target product and the target product is typed by using special analysis software so as to correctly and rapidly diagnose trace HPV virus infection and detect and identify various HPV gene types, such as high-risk type, low-risk type and the like. The method has high sensitivity and wide application range, can be used for detecting various types of samples, such as paraffin sections, formalin-dipped tissues, blood samples, cast-off cells and the like, and is convenient to apply to early screening of cervical cancer.

The present invention discloses methods and devices for rapid genotyping. In one embodiment, the present invention is applied to human papillomavirus (HPV) genotyping, comprising the use of viral genotype-specific-oligonucleotide probes, reversed-dot-blotting genotype array format and flow through hybridization process, thereby providing a more efficient, faster and less expensive method for HPV genotyping. The genotyping method further comprises the use of generic probes to expand the detection of HPV genotypes.

The invention provides a method and a device for calculating a relationship between high-risk HPV types and cervical cancer precancerous lesion stages. The method comprises the steps: carrying out classification on N kinds of high-risk HPV infection data, obtained through thin-layer cytological examination (TCT) and HPV genotyping detection, under M kinds of cervical cancer precancerous lesion stages, and obtaining HPV infection preprocessing data in different infection modes; performing clustering analysis based on the HPV preprocessing data, and obtaining the similarity of different high-risk HPVs based on a clustering analysis result; and carrying out modeling according to Poisson distribution based on the HPV preprocessing data under the single infection and multiple infection modes, and performing regression analysis to obtain the influence proportion of the HPV single infection and multiple infection on the cervical cancer precancerous lesion. According to the method, a clustering technology and a statistical analysis method are combined to mine biological data, and the relationship between different high-risk HPVs and different cervical cancer precancerous lesion stages is found.

Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type.

The invention relates to a kit for rapid detection of HPV genotypes with a reverse dot blotting method. The kit for detection of 28 HPV genotypes which cover 18 HPV high-risk types and 10 HPV low-risk types in total is built. The kit comprises two parts of PCR reagents for HPV typing and reverse dot blotting reagents, the HPV types of clinical samples can be detected rapidly, accurately and in high throughput, the kit has a prediction effect on HPV clinical diagnosis and cervical cancer prognosis, and the problems that in the prior art, detection is time-consuming, specific typing cannot be carried out, and the genotypes classified are few, so that common HPV infection types of countrymen are not covered as many as possible are solved.

Embodiments of the invention provide methods, polyclonal antibodies, monoclonal antibodies, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and / or late HPV-associated or HPV-specific proteins or antibodies. Monoclonal antibodies are used to detect oncogenic high risk and low risk HPV types in a single assay, which is not limited to assay type or format. Useful tools for specific detection of various HPV associated cancers are provided. HPV associated cancer biomarkers are identified and can be used in a screening method for early stage precancerous lesions as well as late stage cancer progression.

The invention discloses a method and a kit for detecting an HPV (Human Papillomavirus) genotype. The method for detecting the HPV genotype comprises the following steps of: A, extracting DNA (Deoxyribonucleic Acid); b, carrying out PCR (Polymerase Chain Reaction) on a DNA template, and amplifying to obtain a PCR product of an HPV-infected DNA sample; and C, carrying out restricted segment length polymorphism analysis through restriction endonuclease. The method is low in detection cost and can be used for rapidly and intuitively determining the type of the infected HPV according to a special HPV type atlas.

The present invention relates to a composition comprising an oligonucleotide mixture. Moreover, the present invention relates to the use of said oligonucleotide mixture for diagnosing different HPV genotypes in a sample of a subject. Further encompassed is a method for diagnosing different HPV genotypes in a sample of a subject and a kit carrying out said method.

The invention relates to the field of in-vitro diagnostic reagent detection, in particular to a full-automatic sample adding, hybridization and detection method for an HPV genotyping chip. The methodcomprises the following steps: dropwise adding a PCR product into a small test tube of a PCR product middle rotating disc, placing an HPV genotyping chip in a large test tube of a biochemical hybridization large rotating disc, preparing a reaction liquid required by hybridization color development and distilled water for cleaning a pipetting device, and carrying out hybridization detection. The detection method has the beneficial effects that rapid and convenient suction and sample adding are realized, the continuity of detection steps is ensured, the workload of manual intervention is relatively small and the detection efficiency is improved.

This invention discloses a method of quickly distinguish and classify existing genital meatus human papilloma virus. The method can distinguish existing 48 type HPV that can infect genital meatus mucous membrane and its normal mixed infection. And it can select Rsa I,Mse I,Pst I and HaeIII four normal using inscribing enzyme combinations according to optimal selection, optimize assort and at the same time the sensitivity and specificity can be ensured. Then HPV gene type is judged according to the established genital HPV gene type MY09 / 11 amplification product RMPH enzyme inscribing data. The operation is simple and time can be saved, the cost is low, its equipment needed exists in county hospital, and its cost is very low. It can be high pass quantity experienced, the technical operability of developing HPV inflection epidemiology investigation.

The invention provides a method for flexibly detecting human papilloma virus genotypes in a broad spectrum. The detected types contain 45 carcinogenic and pathogenic HPV genotypes (subtypes) described by the International Agency for Research on Cancer IARC and one giant wart-inducing type newly authenticated and reported by China CDC, and the coverage exceeds the sum of the types involved by the present various similar kits. According to the method provided by the invention, mass-spectrum scanning multiple PCR is taken as a platform, 21 carcinogenic and 2 high-incidence wart-inducing types with the highest clinic need frequency are detected as an independent reaction, and only two reactions are needed in total, thus further meeting the needs of clinic emphasis detection and difficult and complicated disease broad-spectrum screening; detection combinations are flexible and cost performance is high; several and even thousands of samples can be detected in one day; a result is automatically reported in real time, without the influences of human factors; tens of HPV types can be accurately detected simultaneously in one step. The method embodies the unique advantages of high flux, high flexibility, high automation and wide linear range, and provides an indispensable detection tool for comprehensive universal screening for pathogenic HPV infection.

The invention relates to a detection method for diagnosis of human papilloma virus (HPV) infection, and utilization of a kit prepare by the method for detecting HPV genotype DNA samples isolated from the patient in order to diagnose whether it belongs to one of the 24 types, and belongs to the technical field of life science. The method of the invention comprises a polymerase chain reaction based on PCR technology; the PCR reaction comprises simultaneous detection of DNA of 24 types including HPV6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83, MM4 and cp8304 on a fluorescently-labeled upstream primers and unlabeled degenerated downstream primers of various types under particular PCR condition in a reaction tube, capillary denaturing gel electrophoresis, data read by sequenator, and division into specific types by a piece of provided software directly.

Disclosed are PNA probes capable of genotype specifically binding with Human Paillomavirus (HPV) DNA, kits for detecting HPV genotypes comprising the probes, and methods for detecting HPV genotypes by using the kits, which enables the accurate detection of all 24 genotypes of HPV found in cervix, diagnosis of combined infection with more than one HPV genotype, and detection of HPV genotypes with high specificity and sensitivity.